Neonatal exposure to monosodium l-glutamate induces loss of neurons and cytoarchitectural alterations in hippocampal CA1 pyramidal neurons of adult rats

Beas‐Zárate, Carlos; Vega, M.I. Pérez; González-Burgos, Ignacio

doi:10.1016/s0006-8993(02)03252-3

Cited by 58 publications

(37 citation statements)

References 32 publications

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“…However, some CR-negative neurons were morphologically changed in a way which may suggest neuronal death. Thus, the cell density loss may probably be related to the appearance of excitotoxicity phenomenon, which was suggested by results of previous studies [27][28][29]. It has been established that the excitotoxicity caused by different factors leads to serious neuronal disorders and even death of nervous cells.…”

Section: Discussionmentioning

confidence: 83%

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Calretinin (CR) is a protein, which is present in GABAergic neurons and belongs to the calciumbinding proteins family. It may reduce the excitotoxicity phenomenon through its Ca 2+ buffering properties. This phenomenon is due to the increase of calcium ions levels caused by the excess of glutamate -the main excitatory neurotransmitter. The aim of the study was to investigate alterations of calretinin-immunoreactivity in neurons of hippocampal CA1 region and dentate gyrus with hilus in 10 day-old rats treated with monosodium glutamate (MSG). Material and methods. Ten 7 day-old Wistar rats were used. The MSG-group consisted of 5 MSG-treated rats at a dose of 4 g/kg b.w. for 3 consecutive days and the second group consisted of 5 control animals. After euthanasia the brains containing hippocampus were dissected and embedded in paraffin blocks. The immunohistochemical peroxidase-antiperoxydase reaction was performed on tissue sections. The morphometric analyses of CR-immunopositive neurons: density, percentage ratio to the density of all cells and an assessment of digital immunostaining intensity were performed. Results. The distribution of the CR-immunoreactive neurons in the hippocampus was irregular. In the MSGgroup there were single cells, which were more intensely stained than in control animals. Some of cells contained processes of different length. The density of CR-immunopositive cells and their percentage ratio to the density of all cells did not change significantly after MSG treatment. However, there was a statistically significant increase in the staining intensity of CR-immunopositive cells. Conclusions. The obtained results indicate that CR-positive cells in P7-P10 rats are only slightly affected by MSG in CA1 region and dentate gyrus with hilus of the hippocampus.

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Section: Discussionmentioning

confidence: 83%

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Rycerz

Krawczyk

Jaworska-Adamu

et al. 2015

Folia Histochem Cytobiol.

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“…The hippocampus has an important role in spatial learning and memory [53] . Damage to and morphological changes of the CA1 hippocampal structure has been described in MSG-treated animals [36,54] . Our data confirmed that the CA1 hippocampal structure underwent disintegration.…”

Section: Discussionmentioning

confidence: 99%

“…Effects of AA on MSG-induced injury of the hippocampus Neonatal MSG treatment produces degenerative changes in the developing brain; many studies have found that the injury is attributable to the destruction of the hippocampus [36] . In HE-stained sections, neuronal damage was manifested in the MSG group.…”

Section: Effects Of Aa On Msg-induced Cognitive Deficitsmentioning

confidence: 99%

Asiatic acid, a pentacyclic triterpene in Centella asiatica, attenuates glutamate-induced cognitive deficits in mice and apoptosis in SH-SY5Y cells

et al. 2012

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Aim: To investigate whether asiatic acid (AA), a pentacyclic triterpene in Centella asiatica, exerted neuroprotective effects in vitro and in vivo, and to determine the underlying mechanisms. Methods: human neuroblastoma Sh-SY5Y cells were used for in vitro study. Cell viability was determined with the MTT assay. hoechst 33342 staining and flow cytometry were used to examine the apoptosis. The mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) were measured using fluorescent dye. PGC-1α and Sirt1 levels were examined using Western blotting. Neonatal mice were given monosodium glutamate (2.5 mg/g) subcutaneously at the neck from postnatal day (PD) 7 to 13, and orally administered with AA on PD 14 daily for 30 d. The learning and memory of the mice were evaluated with the Morris water maze test. hE staining was used to analyze the pyramidal layer structure in the CA1 and CA3 regions. Results: Pretreatment of Sh-SY5Y cells with AA (0.1-100 nmol/L) attenuated toxicity induced by 10 mmol/L glutamate in a concentration-dependent manner. AA 10 nmol/L significantly decreased apoptotic cell death and reduced reactive oxygen species (ROS), stabilized the mitochondrial membrane potential (MMP), and promoted the expression of PGC-1α and Sirt1. In the mice models, oral administration of AA (100 mg/kg) significantly attenuated cognitive deficits in the Morris water maze test, and restored lipid peroxidation and glutathione and the activity of SOD in the hippocampus and cortex to the control levels. AA (50 and 100 mg/kg) also attenuated neuronal damage of the pyramidal layer in the CA1 and CA3 regions. Conclusion: AA attenuates glutamate-induced cognitive deficits of mice and protects SH-SY5Y cells against glutamate-induced apoptosis in vitro.

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“…W licznych badaniach wykazano, że systematyczne podawanie soli sodowej kwasu glutaminowego neonatalnym szczurom prowadzi do uszkodzenia struktur nerwowych wielu obszarów OUN (2,6,20,25). Młode zwierzęta są bardziej wrażliwe na toksyczne działanie Glu w porównaniu do osobników dorosłych, co należy wiązać ze stopniem rozwoju i przepuszczalności bariery krew-mózg (blood-brain barrier -BBB).…”

Section: Praca Oryginalnaunclassified

Influence of the age of rats treated with the sodium salt of glutamate acid on the reactivity of astroglia of the infundibular nucleus

Krawczyk¹,

Jaworska-Adamu²

2017

Medycyna Weterynaryjna

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The aim of the study was the immunohistochemical evaluation of the impact of the age of animals treated with the sodium salt of glutamic acid on the behaviour of astrocytes of the infundibular nucleus (IN). Immunohistochemical peroxidase-antiperoxidase reactions were conducted on brain sections of 10-day-old (I) and 63-day-old (II) rats treated s.c with monosodium glutamate (MSG) in a dose of 4g/kg b.w. for three consecutive days. The staining was performed using specific antibodies against glial fibrillary acidic protein (GFAP), S-100β protein and Ki-67 antigen. Cells immunopositive for the proteins under investigation were assessed morphologically and morphometrically in an Olympus BX51 light microscope with the Cell ^ D program. Statistically significant differences were tested using ANOVA and the non-parametric Kruskal-Wallis test. In the infundibular nucleus of 10-day-old rats treated with MSG, there was an increase in the number of GFAP, S-100β and Ki-67 immunopositive astrocytes without any changes in their morphology, which was typical of immature glia. In adult rats treated with MSG, a decrease in the number of cells expressing GFAP and S-100β was found. Most astrocytes had thick and weakly branched processes, in contrast to those observed in control animals. The results of our study showed a diverse behaviour of astroglia of IN in young and adult rats treated with MSG. In 10-day-old rats, hyperplasia of glia occurred, whereas in 63-day-old individuals there was a loss and hypertrophy of astrocytes, which may indicate a late stage of their reactivity. This information may contribute to targeting the therapy of diseases of the nervous system induced by the excitotoxic effects of glutamate.

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Neonatal exposure to monosodium l-glutamate induces loss of neurons and cytoarchitectural alterations in hippocampal CA1 pyramidal neurons of adult rats

Cited by 58 publications

References 32 publications

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Effects of monosodium glutamate treatment on calretinin-immunoreactive neurons in hippocampus of postnatal rats

Asiatic acid, a pentacyclic triterpene in Centella asiatica, attenuates glutamate-induced cognitive deficits in mice and apoptosis in SH-SY5Y cells

Influence of the age of rats treated with the sodium salt of glutamate acid on the reactivity of astroglia of the infundibular nucleus

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