Neonatal Sepsis; Common Bacterial Isolates and Their Antimicrobial Susceptibility Patterns in Neonatal Intensive Care Unit, Islamabad

Abbasi, Nazia Bashir; Jabeen, Nighat; Khatoon, Shafat

doi:10.17957/tpmj/17.3914

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…In the present study, bacterial isolates were tested for antimicrobial susceptibility by Modified Kirby Bauer Disc Diffusion technique according to CLSI guideline 2017 and FDA guideline 2013. Among the 18 isolated Klebsiella spp, all were resistant to Ampicillin, Cefotaxime and Ceftazidime which is similar to a relevant study 34 . In our study, most Klebsiella spp were sensitive to Tigecycline which is concordant to a similar study 35 .…”

Section: Discussionsupporting

confidence: 84%

“…Among the 6 isolates of Acinetobacter spp, all of the isolates (100%) were resistant to Ampicillin and Cefotaxime, followed by 83.4% to Amikacin, Gentamicin and Ceftazidime. Similar resistance against Ampicillin and Cefotaxime were observed in a study in Pakistan 34 and against Ceftazidime, Amikacin, and Gentamicin 85.7% each in a recent Bangladeshi study 11 . Our study showed that Acinetobacter spp were mostly sensitive to Piperacillin-tazobactum (66.66%) which is similar to a recent study 37 .…”

Section: Discussionsupporting

confidence: 77%

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Comparison of Conventional and Automated Blood Culture Methods for The Diagnosis of Neonatal Septicemia

Chowdhury¹,

Akter²,

Ahmed³

et al. 2021

Bangladesh J Med Microbiol

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Neonatal Septicemia is a serious clinical syndrome and the definitive diagnosis is based on positive blood cultures which are obtained either by conventional or automated method. Early availability of proper isolation and identification of causative bacteria facilitates the timely initiation of appropriate antibiotic therapy. Thereby the present study was conducted to identify the bacterial causes of neonatal septicemia in the fastest possible time by comparing conventional and automated blood culture methods.This cross sectional study was done during the period from January 2018 to December 2018 and included clinically suspected cases of neonatal septicemia admitted to Neonatal Intensive Care Units of Chattogram Medical College Hospital (CMCH) and Chattogram Maa-Shishu O General Hospital (CMSOGH). Out of 178 samples, automated method detected 29 (16.3%) and conventional method detected 26 (14.6%) blood culture positive samples. The yield of bacteria by automated method was 100% and by conventional method was 89.7%. Number of bacteria isolated only by automated method were 3 (10.2%). Mean time for isolation of bacteria by automated method was 26.38 hours and by conventional method was 46.34 hours. Automated method detected 47.05% of isolated bacteria in first 24 hours but none of them were detected by conventional method within first 24 hours. Among the isolated bacteria, Klebsiella spp was most common (62.0%). Most of the isolates were resistant to Ampicillin, Cefotaxime and Ceftazidime. Analyzing the findings of the study, there was no significant difference in the rate of isolation in each time interval (p=0.157) of two methods but there was significant difference in the mean time of isolation of bacteria between two methods (p=0.000004). Bangladesh J Med Microbiol 2021; 15 (2): 12-18

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Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 77%

Comparison of Conventional and Automated Blood Culture Methods for The Diagnosis of Neonatal Septicemia

Chowdhury¹,

Akter²,

Ahmed³

et al. 2021

Bangladesh J Med Microbiol

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“…11 Similar patterns of pathogenic isolations were seen in a study in Islamabad including dominant isolation of Klebsiella followed by Acinetobacter, Ecoli, and Pseudomonas. 12 Klebsiella was also the principal pathogen isolated from positive blood cultures of neonates in India. 13 In a study conducted in Sialkot, Klebsiella pneumonae and E-coli were gram-negative bacteria, and Staphylococcus aureus (12.2%) causing the late onset of disease was the most common gram-positive organism isolated.…”

Section: Discussionmentioning

confidence: 99%

Neonatal sepsis and resistance pattern of isolates in Tertiary level neonatal unit: Time to evaluate the empirical antibiotics selection

Ehtizaz¹,

Haider²,

Fayyaz³

et al. 2021

JRMC

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Objective: To find out the most common organisms involved in neonatal sepsis origination and observe the pattern of antibiotic sensitivity and resistance of bacterial isolates. Materials and Methods: This descriptive cross-sectional study was conducted at the Department of Paediatrics Izzat Ali Shah Hospital, Wah Cantt. Out of 420 patients admitted with sepsis in NICU, 19.5% of patients with positive blood cultures were included in the study. A consecutive, non-probability sampling technique was used. Results: Out of 82 positive blood cultures gram-positive bacteria were observed in 19 patients (23.2%) and gram-negative bacteria were seen in 63 patients (76.8%). The most common gram-negative pathogens isolated were Acinetobacter (29.3%) and Klebsiella (24.4%). Staphylococcus aureus (12.2%) was the commonest gram-positive organism isolated. Gram-negative organisms showed maximum sensitivity to Tigecycline and Colistin and were resistant to Cefixime, Aztreonam, Amoxicillin, and Ceftriaxone. Gram-positive bacteria were sensitive to Teicoplanin, Linezolid, and Vancomycin while resistance was shown to penicillin and amoxicillin.Conclusion: The current study showed that gram-negative bacteria were the major contributors to sepsis in the respective setup and showed resistance to first-line antibiotics such as Penicillins and Cephalosporins. Strict infection control measures need to be implemented to avoid the emergence of resistant strains of pathogens in NICUs. This will help to reduce the incidence of neonatal sepsis leading to mortality.

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“…2 A high index of suspicion, along with diagnostic tests, is required since features of sepsis in neonates are nonspecific and ill-defined. 3 Most babies admitted to the neonatal intensive care unit either require antibiotics or are administered antibiotics for one reason. 4,5 Although blood culture is the gold standard for diagnosing neonatal sepsis, chances of getting a truly positive blood culture in neonates are minimal due to multiple reasons mentioned in the studies done in the past.…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Lymphocyte Ratio as Screening Tool for Neonatal Sepsis in Developing Countries

Naseer

Sheikh²,

Zulfiqar³

et al. 2023

PAFMJ

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Objective: to recognize an alternate method for screening neonatal sepsis in patients while considering the sensitivity and accuracy of the diagnosis. Study Design: Cross sectional study. Place and Duration of Study: Combined Military Hospital, Rawalpindi Pakistan, from Jun to Dec 2021. Methodology: Patients admitted to the Neonatal Intensive Care Unit of the Combined Military Hospital were included in the study. Demographic data, including age, weight, gender, and gestational age, were recorded. In addition, a statistical comparison of total leucocyte count, absolute neutrophil and lymphocyte counts, neutrophil-lymphocyte ratio, and C-reactive protein test was carried out for the cases and controls. Results: It was evident from the results that a significant correlation between the neutrophil-lymphocyte ratio and neonatal sepsis exists. The mean NLR in non-septic individuals was (1.8±1.3) and in septic individuals, it was (2.9±1.9). The p-value was 0.001. Furthermore, ROC showed that at an NLR value of 1.6, sensitivity was 0.594 and 1-specificity 0.295. Conclusion: Neutrophil lymphocyte ratio was an efficient screening tool for determining sepsis in neonates, with comparable results to the C-reactive protein test.

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Neonatal Sepsis; Common Bacterial Isolates and Their Antimicrobial Susceptibility Patterns in Neonatal Intensive Care Unit, Islamabad

Cited by 3 publications

References 10 publications

Comparison of Conventional and Automated Blood Culture Methods for The Diagnosis of Neonatal Septicemia

Comparison of Conventional and Automated Blood Culture Methods for The Diagnosis of Neonatal Septicemia

Neonatal sepsis and resistance pattern of isolates in Tertiary level neonatal unit: Time to evaluate the empirical antibiotics selection

Neutrophil Lymphocyte Ratio as Screening Tool for Neonatal Sepsis in Developing Countries

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