Neonicotinoid insecticides imidacloprid and clothianidin affect differently neural Kenyon cell death in the cockroach Periplaneta americana

Benzidane, Yassine; Lapied, Bruno; Thany, Steeve Hervé

doi:10.1016/j.pestbp.2011.09.005

Cited by 28 publications

(10 citation statements)

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“…A similar range of neonicotinoid concentrations (up to 100-300 mg/l) was measured in guttation drops of corn seedlings from coated seeds (Girolami et al, 2009;Tapparo et al, 2011). Compared with other laboratory studies, the concentration (300-400 µM) of neonicotinoids in our experiments represent the upper range of the values used in insect experiments (in vitro and in vitro), which varied from 1-10 nM (Thany, 2009;Benzidane et al, 2011;Palmer et al, 2013), up to as high as 1 mM (Deglise et al, 2002).…”

Section: Discussionsupporting

confidence: 82%

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

et al. 2015

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Neonicotinoids are highly potent and selective systemic insecticides, but their widespread use also has a growing impact on non-target animals and contaminates the environment, including surface waters. We tested the neonicotinoid insecticides commercially available in Hungary (acetamiprid, Mospilan; imidacloprid, Kohinor; thiamethoxam, Actara; clothianidin, Apacs; thiacloprid, Calypso) on cholinergic synapses that exist between the VD4 and RPeD1 neurons in the central nervous system of the pond snail Lymnaea stagnalis. In the concentration range used (0.01-1 mg/ml), neither chemical acted as an acetylcholine (ACh) agonist; instead, both displayed antagonist activity, inhibiting the cholinergic excitatory components of the VD4-RPeD1 connection. Thiacloprid (0.01 mg/ml) blocked almost 90 % of excitatory postsynaptic potentials (EPSPs), while the less effective thiamethoxam (0.1mg/ml) reduced the synaptic responses by about 15 %. The ACh-evoked membrane responses of the RPeD1 neuron were similarly inhibited by the neonicotinoids, confirming that the same ACh receptor (AChR) target was involved. We conclude that neonicotinoids act on nicotinergic acetylcholine receptors (nAChRs) in the snail CNS. This has been established previously in the insect CNS; however, our data indicate differences in the background mechanism or the nAChR binding site in the snail. Here we provide the first results concerning neonicotinoid-related toxic effects on the neuronal connections in the molluscan nervous system. Aquatic animals, including molluscs, are at direct risk while facing contaminated surface waters, and snails may provide a suitable model for further studies of the behavioural/neuronal consequences of intoxication by neonicotinoids.Tihany, Hungary, July 22, 2015 Dr. Mikko Nikinmaa Editorial Office Aquatic ToxicologyDear Dr Nikinmaa, Thank you very much for your response to our manuscript submission. We gratefully thank you and our reviewers for reading the manuscript, and we appreciate all the helpful comments made.After carefully checking the Reviewers' comments we improved the manuscript on the basis of their suggestions. Please find our detailed answers to the Reviewers below. Reviewer #1:We greatly appreciate the Reviewer's comment "one of the best written manuscripts that I have reviewed" Minor points Materials and Methods How was Lymnaea saline obtained? Is this the modified HiDi saline? If not, what is HiDi?For isolating the monosynaptic components of the synaptic responses between two neurons the perfusion chamber was filled with a modified saline which reduces the polysynaptically evoked postsynaptic responses and enhances the monosynaptic component (Berry and Pentreath, 1976). This modified saline contains higher amount of divalent cations (Ca 2+ and Mg 2+ ), therefore often nicknamed as HiDi saline (Brierly et al., 1997, Sivaramakrishnan et al., 2013. We added details to the text accordingly Electrophysiological recordingThere is a Winlow 1991 anda Syed et al. 1990 (PNEC, EC50, mechanistic...) We...

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Section: Discussionsupporting

confidence: 82%

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

et al. 2015

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“…Patch clamp recordings were performed on adult cockroach Kenyon cells. Kenyon cells were isolated as previously described (Benzidane et al., ). The brain was removed from the head capsule and placed in saline solution (in mM: NaCl, 200; KCl, 3.1; CaCl 2 , 5; MgCl 2 , 4; HEPES, 10; saccharose, 50; pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE‐INDUCED CURRENTS

Taillebois

Thany

2016

Arch Insect Biochem Physiol

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Nicotinic acetylcholine receptors are ligand-gated ion channels expressed in many insect structures, such as mushroom bodies, in which they play a central role. We have recently demonstrated using electrophysiological recordings that different native nicotinic receptors are expressed in cockroach mushroom bodies Kenyon cells. In the present study, we demonstrated that eight genes coding for cockroach nicotinic acetylcholine receptor subunits are expressed in the mushroom bodies. Quantitative real-time polymerase chain reaction (PCR) experiments demonstrated that β1 subunit was the most expressed in the mushroom bodies. Moreover, antisense oligonucleotides performed against β1 subunit revealed that inhibition of β1 expression strongly decreases nicotine-induced currents amplitudes. Moreover, co-application with 0.5 μM α-bungarotoxin completely inhibited nicotine currents whereas 10 μM d-tubocurarine had a partial effect demonstrating that β1-containing neuronal nicotinic acetylcholine receptor subtypes could be sensitive to the nicotinic acetylcholine receptor antagonist α-bungarotoxin.

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“…Studies evaluating the effects of neonicotinoids on continued nerve transmission across the synapses have identified different binding properties. Imidacloprid is a partial agonist of the nAChR that binds to the acetylcholine (ACh) recognition site (Barbara et al, 2008;Barbara et al, 2003;Benzidane et al, 2011;Deglise et al, 2002). Clothianidin was found to be a full agonist in cockroaches (Periplaneta americana) but this has not been confirmed in honeybees (Benzidane et al, 2011).…”

Section: Mode Of Action and Metabolism Of Neonicotinoid Insecticidesmentioning

confidence: 99%

“…Imidacloprid is a partial agonist of the nAChR that binds to the acetylcholine (ACh) recognition site (Barbara et al, 2008;Barbara et al, 2003;Benzidane et al, 2011;Deglise et al, 2002). Clothianidin was found to be a full agonist in cockroaches (Periplaneta americana) but this has not been confirmed in honeybees (Benzidane et al, 2011). Nitenpyram, despite having a neonicotinoid structure, behaves more like nicotine, and was observed that it binds to both the ACh site and an allosteric site within the nAChR (Tomizawa and Yamamoto, 1993).…”

Section: Mode Of Action and Metabolism Of Neonicotinoid Insecticidesmentioning

confidence: 99%

Toxicity of Neonicotinoids to Honey Bees and Detoxification Mechanism in Honey Bees

Magesh¹,

Zhu²,

Tang³

et al. 2017

IOSR JESTFT

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Neonicotinoid insecticides imidacloprid and clothianidin affect differently neural Kenyon cell death in the cockroach Periplaneta americana

Cited by 28 publications

References 52 publications

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

CHARACTERIZATION OF NICOTINE ACETYLCHOLINE RECEPTOR SUBUNITS IN THE COCKROACH Periplaneta americana MUSHROOM BODIES REVEALS A STRONG EXPRESSION OF β1 SUBUNIT: INVOLVEMENT IN NICOTINE‐INDUCED CURRENTS

Toxicity of Neonicotinoids to Honey Bees and Detoxification Mechanism in Honey Bees

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