Nephrin localizes to the slit pore of the glomerular epithelial cell

Holzman, Lawrence B.; John, Patricia L. St.; Kovari, Iulia A.; Verma, Rakesh; Holthöfer, Harry; Abrahamson, Dale R.

doi:10.1046/j.1523-1755.1999.00719.x

Cited by 285 publications

(247 citation statements)

References 12 publications

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“…3 Nephrin is similarly modified when expressed in MDCK cells, because treatment of cell lysates with N-glycanase (removes N-linked oligosaccharides) resulted in a shift in the electrophoretic mobility of nephrin from ϳ180 to 185 to ϳ150 to 160 kd ( Figure 1C).…”

Section: Nephrin Is N-glycosylated and Phosphorylated In Mdck-nephrinmentioning

confidence: 99%

“…Thus the distribution of nephrin in MDCK cells resembles that in the glomerulus where nephrin has been localized in both the junctional or slit diaphragm region and at the apical domain of podocytes. [3][4][5] …”

Section: Nephrin Is Present On Both Apical and Lateral Domains Of Thementioning

confidence: 99%

“…As already mentioned, in podocytes nephrin was found both at the slit diaphragm and along the apical plasma membrane. [3][4][5] …”

Section: Nephrin Partially Co-localizes With Junctional Proteins In Mmentioning

confidence: 99%

“…1 NPHS1 encodes for nephrin, 1 a member of the immunoglobulin (Ig) superfamily of cell adhesion molecules, 2 which is localized in the glomerular slit diaphragm and has been suggested to form the framework of this specialized cell adhesion structure. [3][4][5] Slit diaphragms are important components of the glomerular filtration barrier. They bridge the urinary spaces between the foot processes of the glomerular epithelium and serve to attach foot processes to one another.…”

mentioning

confidence: 99%

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Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin

Lehtonen

Kudlicka

Holthöfer

et al. 2004

The American Journal of Pathology

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Section: Nephrin Is N-glycosylated and Phosphorylated In Mdck-nephrinmentioning

confidence: 99%

Section: Nephrin Is Present On Both Apical and Lateral Domains Of Thementioning

confidence: 99%

“…As already mentioned, in podocytes nephrin was found both at the slit diaphragm and along the apical plasma membrane. [3][4][5] …”

Section: Nephrin Partially Co-localizes With Junctional Proteins In Mmentioning

confidence: 99%

mentioning

confidence: 99%

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Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin

Lehtonen

Kudlicka

Holthöfer

et al. 2004

The American Journal of Pathology

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“…The podocytes form primary and secondary foot processes, which interdigitate to form a microscopic filtration structure, the slit diaphragm. A key component of the slit is a transmembrane protein, nephrin [9]. Two major mutations in the nephrin gene lead to a massively proteinuric disease, congenital nephrotic syndrome of the Finnish type [10].…”

Section: Introductionmentioning

confidence: 99%

A 100-kDa urinary protein is associated with insulin resistance in offspring of type 2 diabetic patients

et al. 2005

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Aims/hypothesis: One-third of normoalbuminuric type 1 diabetic patients show immunoreactive nephrin in urine. Offspring of type 2 diabetic patients are insulinresistant and susceptible to the development of diabetes. We investigated whether the offspring of type 2 diabetic patients show nephrin in urine and whether possible nephrinuria is associated with insulin resistance. Methods: : Urinary proteins from timed overnight urine collections from 128 offspring of type 2 diabetic patients and 9 control subjects were analysed by western blotting using an antibody against nephrin. Glucose metabolism was assessed by OGTT and IVGTT and the euglycaemic-hyperinsulinaemic clamp technique. Results: Of the offspring, 12.5% were strongly and 14.1% weakly positive for a 100-kDa urinary protein. All controls were negative. During the first 10 min of an IVGTT, the offspring strongly positive for the urinary protein had a higher inysulin response than the offspring without the protein (3,700 vs 2,306 pmol l −1 min −1 , p=0.007). Insulin sensitivity (the rate of wholebody glucose uptake divided by the steady-state insulin level×100) was lower among the offspring strongly positive for the urinary protein than among the offspring negative for the protein (11.3 vs 15.8 μmol kg −1 min −1 pmol −1 l −1 , p=0.008). Conclusions/interpretation: A 100-kDa urinary protein detectable with a nephrin antibody is associated with insulin resistance in offspring of type 2 diabetic patients.

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Nephrin

Holthöfer¹

2002

Wiley Encyclopedia of Molecular Medicine

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Nephrin localizes to the slit pore of the glomerular epithelial cell

Abstract: As a putative cell adhesion molecule of the immunoglobulin superfamily, nephrin likely participates in cell-cell interactions between podocyte foot processes and may represent a component of the slit diaphragm.

Cited by 285 publications

References 12 publications

Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin

Nephrin Forms a Complex with Adherens Junction Proteins and CASK in Podocytes and in Madin-Darby Canine Kidney Cells Expressing Nephrin

A 100-kDa urinary protein is associated with insulin resistance in offspring of type 2 diabetic patients

Nephrin

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