Nephro-Protective Effect of the Indonesian Propolis Extract on Unilateral Renal Ureter Obstructive Damage

Abstract

Background: Ureter obstruction can cause permanent renal damage. In theory, the Indonesian propolis extract (IPE) may play an important role as an antioxidant and anti-inflammatory agent. This study aimed to determine the nephroprotective effect of IPE in reducing interstitial fibrosis, systolic blood pressure (SBP) and increasing body weight in mice with unilateral ureteric obstruction (UUO). Subjects and Method: This study was a quasi experiment. A total sample of 32 mice was allocated into four groups: (1) Mice without UUO; (2) Mice with UUO; (3) Mice with UUO privided with IPE (orally 50mg/ kgBW); (4) Mice with UUO provided with IPE (orally 100mg/ kgBW). After 30 days of intervention, the mice were killed for interstitial fibrosis analysis. Mean differences in body weight, sistolic blood pressure (SBP), and interstitial fibrosis were tested by one way ANNOVA and Kruskal Wallis. Results: Interstitial fibrosis and SBP were higher in group 2 than group 1 resulting from UUO (p< 0.05). Body weight was lower in group 2 than group 1 resulting from UUO (p< 0.05). Interstitial fibrosis and SBP were lower in both group 3 and 4 than group 2 (p< 0.05), indicating that IPE was effective in reducing interstitial fibrosis and SBP. Body weight was higher in group 3 and 4 than group 2 (p< 0.05), indicating that IPE was effective in increasing body weight. Conclusion: IPE can effectively reduce interstitial fibrosis and SBP in mice, and increase body weight.