Nephrocyte-neurocyte interaction and cellular metabolic analysis on membrane-integrated microfluidic device

Zhuang, Qichen; Wang, Shiqi; Zhang, Jie; He, Ziyi; Li, Haifang; Ma, Yuan; Lin, Jin‐Ming

doi:10.1007/s11426-015-5453-3

Cited by 16 publications

(6 citation statements)

References 29 publications

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“…Culturing cells at single‐cell and population levels has provided the opportunity to gain deeper insight into single‐cell behaviour, metabolism mechanisms [165] cell‐cell interactions [141], cell signalling pathway [96], cancer cell metastasis and cell migration. The capability to define the manifestation of cell behaviour through a high spatiotemporal microenvironment is essential to investigate the interplay mechanism between the cells.…”

Section: Applications Of Microfluidic Cell Culture Systemmentioning

confidence: 99%

Recent advances of integrated microfluidic suspension cell culture system

Kerk

Jameel

Xing

et al. 2021

Engineering Biology

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Microfluidic devices with superior microscale fluid manipulation ability and large integration flexibility offer great advantages of high throughput, parallelisation and multifunctional automation. Such features have been extensively utilised to facilitate cell culture processes such as cell capturing and culturing under controllable and monitored conditions for cell‐based assays. Incorporating functional components and microfabricated configurations offered different levels of fluid control and cell manipulation strategies to meet diverse culture demands. This review will discuss the advances of single‐phase flow and droplet‐based integrated microfluidic suspension cell culture systems and their applications for accelerated bioprocess development, high‐throughput cell selection, drug screening and scientific research to insight cell biology. Challenges and future prospects for this dynamically developing field are also highlighted.

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Section: Applications Of Microfluidic Cell Culture Systemmentioning

confidence: 99%

Recent advances of integrated microfluidic suspension cell culture system

Kerk

Jameel

Xing

et al. 2021

Engineering Biology

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“…Dendrites from each side that extended into the microgrooves were shown to facilitate exosome transfer. Another incarnation of a microfluidic coculture system used a permeable polycarbonate membrane to separate neurocytes (PC12) from nephrocytes (293 cells) . In this device, soluble factors from the differentiated PC12 cells were shown to diffuse through the membrane to stimulate the secretion of epinephrine from the 293 cells.…”

Section: Cell Culturing and Analysis Of Intact Cellsmentioning

confidence: 99%

Cellular Analysis Using Microfluidics

et al. 2017

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The papers included in this Review were published between September 2015 and September 2017. The material was compiled using several strategies including extensive searches using Scifinder, Web of Science, PubMed, and Google Scholar. The contents of high impact journals were also scanned, including Analytical Chemistry, Lab-on-a-Chip, Nature, Proceedings of the National Academy of Sciences, Applied Physics Letters, Biosensors and Bioelectronics, Angewandte Chemie, Nano Letters, Science, Biomedical Microdevices, RSC Advances, Electrophoresis, and Langmuir. Several hundred papers relating to the analysis of cells using microfluidics were examined. We have attempted to identify some of the most interesting and promising papers for this Review. Without a doubt, we have missed a few excellent papers and had to eliminate others based on space constraints

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“…The 3D structure provides the foundation of human complex tissue network and is proper to serve as a study model in microfluidics. Other designs of chips for cell-cell interactions include microvalves, microchannels and membrane as shown in reference [63][64][65][66][67][68] (Figure 2b-d) and to control administration of metabolites from one kind of cells to the receptor cells, our group had once presented a so called surface tension plug on a microfluidic device. Based on this device, we investigated the signal pathway between 293 and L-02 cells [69].…”

Section: Gradient Of Chemical Factorsmentioning

confidence: 99%

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

Yi¹,

Lin²

2016

Mol Biol

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Development of microfluidic culture technology combined with tissue engineering catalyzes the progress in study of cell biology. These tools will promote the understanding of physiological and pathological changes. Cancer cells and stem cells are sensitive to their surroundings, thus could be better explored by controllable microfluidic devices. In this review, we describe the ways to control cell microenvironment and explain how the influencing factors influence cellular behaviors, then present microfluidic-chip-based exemplary applications for cancer models and stem cell differentiation.

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Nephrocyte-neurocyte interaction and cellular metabolic analysis on membrane-integrated microfluidic device

Cited by 16 publications

References 29 publications

Recent advances of integrated microfluidic suspension cell culture system

Recent advances of integrated microfluidic suspension cell culture system

Cellular Analysis Using Microfluidics

Development and Applications of Microfluidic Devices for Cell Culture in Cell Biology

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