Nerve grafts

Bunnell, Sterling; Boyes, Joseph H.

doi:10.1016/s0002-9610(39)90934-7

Cited by 76 publications

(13 citation statements)

References 5 publications

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“…In a number of situations, nerve grafting is necessary to transpose defects ranging from a few millimeters to several centimeters in length. The use of a free autogenous nerve graft has long been proposed by many authors, but in the past nerve trunks were used as grafting material and reported good functional results were seldom above 50% of normal (Bunnell and Boyes, 1939;Sanders, 1942;Brooks, 1955). The technique of microsurgical multiple cable autogenous nerve grafting was introduced in the early 1970s (Millesi et al, 1972) and soon became of almost universal acceptance, being applied by most surgeons with small variations and generally good functional results.…”

Section: Introductionmentioning

confidence: 99%

The use of a muscle graft to repair a segmentary nerve defect

Oliveira

Mazzer

Barbieri

et al. 2004

Journal of Neuroscience Methods

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Section: Introductionmentioning

confidence: 99%

The use of a muscle graft to repair a segmentary nerve defect

Oliveira

Mazzer

Barbieri

et al. 2004

Journal of Neuroscience Methods

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“…To increase the prospects of axonal regeneration and functional recovery, numerous strategies have been used, such as implantation of autografts, allografts, xenografts, and Schwann cell-filled tubes. [3][4][5] Although autografts are the clinically ideal graft type, they pose the disadvantages of limited availability and the sacrifice of a healthy nerve, which causes permanent denervation of the donor site. Axonal regeneration through Schwann cell-filled tubes has shown success in a number of animal models; however, the isolation and expansion of Schwann cells for the treatment of nerve injury in humans have proven to be difficult.…”

Section: Introductionmentioning

confidence: 99%

Laminin-coated poly(L-lactide) filaments induce robust neurite growth while providing directional orientation

Rangappa¹,

Romero²,

Nelson³

et al. 2000

J. Biomed. Mater. Res.

113

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Cellular channels during development and after peripheral nerve injury are thought to provide guidance cues to growing axons. In tissue culture where these cues are absent, neurites from dorsal root ganglion neurons grow with a radial distribution. To induce directional axonal growth and to enhance the rate of axonal growth after injury, we have designed microfilaments of poly(L-lactide). We demonstrate that dorsal root ganglia grown on these filaments in vitro extend longitudinally oriented neurites in a manner similar to native peripheral nerves. The extent of neurite growth was significantly higher on laminin-coated filaments compared with uncoated and poly-L-lysine-coated filaments. As high as 5.8 +/- 0.2 mm growth was observed on laminin-coated filaments compared with 2.0 +/- 0.2 mm on uncoated and 2.2 +/- 0.3 mm on poly-L-lysine-coated filaments within 8 days. Schwann cells were found to grow on all types of filaments. They were, however, absent in the leading edges of growth on laminin-coated filaments. Photolysis of Schwann cells caused a significant reduction in the neurite length on all types of filaments. Laminin-coated filaments, however, induced significantly longer neurites compared with uncoated and/or poly-L-lysine-coated filaments even in the absence of Schwann cells. Our results suggest that laminin-coated poly(L-lactide) filaments are suitable for inducing directional and enhanced axonal growth. Implants designed by arranging these microfilaments into bundles should aid regenerating axons by providing guidance cues and channels to organize matrix deposition, cell migration, axon growth, and improve functional recovery.

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“…They were transplanted to a nerve defect in the sciatic nerve of syngeneic recipient rats. At 2,4,6,8,12,16, and 24 weeks after operation, the sciatic nerves were biopsied and processed for evaluation of choline acetyltransferase (CAT) activity, histological studies, and measurement of wet weight of the muscle innervated by the sciatic nerve. Electrophysiological evaluation of the grafted nerve was also performed before sacrifice.…”

mentioning

confidence: 99%

Experimental study of vascularized nerve graft: Evaluation of nerve regeneration using choline acetyltransferase activity

et al. 2001

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A comparative study of nerve regeneration was performed on vascularized nerve graft (VNG) and free nerve graft (FNG) in Fischer strain rats. A segment of the sciatic nerve with vascular pedicle of the femoral artery and vein was harvested from syngeneic donor rat for the VNG group and the sciatic nerve in the same length without vascular pedicle was harvested for the FNG group. They were transplanted to a nerve defect in the sciatic nerve of syngeneic recipient rats. At 2, 4, 6, 8, 12, 16, and 24 weeks after operation, the sciatic nerves were biopsied and processed for evaluation of choline acetyltransferase (CAT) activity, histological studies, and measurement of wet weight of the muscle innervated by the sciatic nerve. Electrophysiological evaluation of the grafted nerve was also performed before sacrifice. The average CAT activity in the distal to the distal suture site was 383 cpm in VNG and 361 cpm in FNG at 2 weeks; 6,189 cpm in VNG and 2,264 cpm in FNG at 4 weeks; and 11,299 cpm in VNG and 9,424 cpm in FNG at 6 weeks postoperatively. The value of the VNG group was statistically higher than that of the FNG group at 4 weeks postoperatively. Electrophysiological and histological findings also suggested that nerve regeneration in the VNG group was superior to that in the FNG group during the same period. However, there was no significant difference between the two groups after 6 weeks postoperatively in any of the evaluations. The CAT measurement was useful in the experiments, because it was highly sensitive and reproducible.

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Nerve grafts

Cited by 76 publications

References 5 publications

The use of a muscle graft to repair a segmentary nerve defect

The use of a muscle graft to repair a segmentary nerve defect

Laminin-coated poly(L-lactide) filaments induce robust neurite growth while providing directional orientation

Experimental study of vascularized nerve graft: Evaluation of nerve regeneration using choline acetyltransferase activity

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