Nerve Growth Factor- and Neurotrophin-3-Releasing Guidance Channels Promote Regeneration of the Transected Rat Dorsal Root

Bloch, Jocelyne; Fine, Eric G.; Bouché, Nicolas; Zurn, Anne D.; Aebischer, Patrick

doi:10.1006/exnr.2001.7778

Cited by 108 publications

(69 citation statements)

References 42 publications

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“…The limitations in these approaches stem from several factors, like the restrictive cell response owing to activated astrocytes and macrophages [11,28,59]. The long-gap (>4 mm) dystrophic effect, especially for neurons or axons penetrating the CNS-PNS transition zone [57,60], or the leakage or death of grafted cells before they can positively influence the environment [59,60], have been identified as key issues as well. The lack of porosity in the proposed devices, representing a barrier for the exchange of nutrients and cytokines, has been also identified as a cause of failure [63].…”

Section: Discussionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

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Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-L-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10 days.The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5 mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system.

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Section: Discussionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

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“…Stability is another important property of pEVA for drug delivery over an extended period. It has been used in nerve growth factor (NGF)-and NT-3-releasing guidance channels to induce regeneration in transected rat dorsal root (Bloch et al 2001 ).…”

Section: Materials Properties and Methods For Drug Deliverymentioning

confidence: 99%

“…In addition to NGF, NT-3 functions in neurogenesis through promoting the differentiation of new neurons. Moreover, studies have shown that NT-3 can promote cell survival and neurite outgrowth in motor neurons after spinal cord injury (Bloch et al 2001 ;Grill et al 1997 ).…”

Section: Drug Selectionmentioning

confidence: 99%

Bioactive Nanomaterials for Neural Engineering

et al. 2016

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“…It has been extensively used for drug delivery applications, [36][37][38][39] while more recently it has been investigated for neural tissue engineering applications, such as repair of peripheral nerve injury. [40][41][42] Poly (glycolic acid)/Poly (lactic acid)/Poly (lactic-coglycolic acid): Poly (glycolic acid) (PGA) and poly (lactic acid) (PLA) are biodegradable synthetic polymers, which can be reacted to form the copolymer poly (lactic-co-glycolic acid). [43][44][45] After implantation, the ester bonds in the backbone of the polymer can be hydrolyzed into by-products.…”

Section: Examples Of Such Materials Arementioning

confidence: 99%