Nerve injury-induced upregulation of miR-21 in the primary sensory neurons contributes to neuropathic pain in rats

Sakai, Atsushi; Suzuki, Hidenori

doi:10.1016/j.bbrc.2013.04.089

Cited by 88 publications

(82 citation statements)

References 44 publications

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“…Only three of the miRNAs that were expressed abundantly (rno-miR-30d-5p, rnomiR-125b-5p) or moderately (rno-miR-379-5p) were regulated differentially, making them candidates for novel peripheral nervous system determinants of neuropathic pain. Downregulation of miR-7a is causally involved in maintenance of neuropathic pain through regulation of neuronal excitability, and miR-7a replenishment offers a novel therapeutic strategy specific for chronic neuropathic pain [82]. While monitoring higher expression of miR-21 in injured neurons during chronic neuropathic pain state both mechanical allodynia and thermal hyperalgesia attenuated by intrathecal administration of miR-21 inhibitor [82].…”

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

“…Downregulation of miR-7a is causally involved in maintenance of neuropathic pain through regulation of neuronal excitability, and miR-7a replenishment offers a novel therapeutic strategy specific for chronic neuropathic pain [82]. While monitoring higher expression of miR-21 in injured neurons during chronic neuropathic pain state both mechanical allodynia and thermal hyperalgesia attenuated by intrathecal administration of miR-21 inhibitor [82]. Specific ligation of the left fifth lumbar spinal nerve-induced neuropathic pain showed miR-21 expression in the injured DRG neurons, but not neighboring uninjured DRG neurons, was persistently upregulated following the pain development [63,82].…”

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

“…While monitoring higher expression of miR-21 in injured neurons during chronic neuropathic pain state both mechanical allodynia and thermal hyperalgesia attenuated by intrathecal administration of miR-21 inhibitor [82]. Specific ligation of the left fifth lumbar spinal nerve-induced neuropathic pain showed miR-21 expression in the injured DRG neurons, but not neighboring uninjured DRG neurons, was persistently upregulated following the pain development [63,82]. MiR-21 is specifically upregulated in the injured DRG neurons and causally involved in the late phase of neuropathic pain.…”

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

“…In addition, thermal hyperalgesia and mechanical allodynia could attenuate by administration of miR21 inhibitor. Discovering the specific roles of microRNAs in chronic pain has future implications in the creation of micro-RNA related drugs to alleviate pain [63,82].…”

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

See 3 more Smart Citations

Practical Considerations of Convection Enhanced Delivery for Novel Therapies Treating Spinal Cord Injury Related Neuropathic Pain

2017

ARC Journal of Neuroscience

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Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

Section: Mirnas Therapy For Treating Neuropathic Painmentioning

confidence: 99%

See 2 more Smart Citations

Practical Considerations of Convection Enhanced Delivery for Novel Therapies Treating Spinal Cord Injury Related Neuropathic Pain

2017

ARC Journal of Neuroscience

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“…Earlier studies have shown that peripheral inflammation and nerve injury initiate changes in the expression of some miRNAs and Kcna2 AS RNA in DRG [10][11][12][13] and these changes might be responsible for inflammation/nerve injury-induced alterations of some painassociated genes, an increase in DRG neuronal excitability, and pain hypersensitivity [11,13]. Recent evidence indicates that ncRNAs might be the pivotal player in the mechanisms that underlie the development and maintenance of chronic pain.…”

Section: Role Of Mirnas In Chronic Painmentioning

confidence: 99%

miRNAs being Key Contributors to Chronic Pain Development and Maintenance

2017

IJSR

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miRNAs, due to their regulatory role in protein expression have a key role in a number of physiological and pathophysiological processes associated with peripheral and central sensitization mechanisms in the nervous system after nociceptive insults. Maladaptive changes in the levels of these proteins may contribute to abnormal synaptic transmission and neuronal intracellular signalling underlying the onset and development of neuropathic pain. The potential of miRNA profiles to serve as biomarkers of pain is emphasized considering the involvement of inflammatory mediators in inflammation-induced changes in miRNA expression. Such changes are considered to contribute to chronic pain development and maintenance. Therefore, miRNAs may be potential targets for the prevention and/or treatment of chronic inflammatory pain. Understanding of these mechanisms may enable the development of novel therapeutic strategies for preventing and/or treating chronic pain.

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Traumatic brain injury increases levels of miR‐21 in extracellular vesicles: implications for neuroinflammation

et al. 2016

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Traumatic brain injury (TBI) is an important health concern and effective treatment strategies remain elusive. Understanding the complex multicellular response to TBI may provide new avenues for intervention. In the context of TBI, cell–cell communication is critical. One relatively unexplored form of cell–cell communication in TBI is extracellular vesicles (EVs). These membrane‐bound vesicles can carry many different types of cargo between cells. Recently, miRNA in EVs have been shown to mediate neuroinflammation and neuronal injury. To explore the role of EV‐associated miRNA in TBI, we isolated EVs from the brain of injured mice and controls, purified RNA from brain EVs, and performed miRNA sequencing. We found that the expression of miR‐212 decreased, while miR‐21, miR‐146, miR‐7a, and miR‐7b were significantly increased with injury, with miR‐21 showing the largest change between conditions. The expression of miR‐21 in the brain was primarily localized to neurons near the lesion site. Interestingly, adjacent to these miR‐21‐expressing neurons were activated microglia. The concurrent increase in miR‐21 in EVs with the elevation of miR‐21 in neurons, suggests that miR‐21 is secreted from neurons as potential EV cargo. Thus, this study reveals a new potential mechanism of cell–cell communication not previously described in TBI.

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Nerve injury-induced upregulation of miR-21 in the primary sensory neurons contributes to neuropathic pain in rats

Cited by 88 publications

References 44 publications

Practical Considerations of Convection Enhanced Delivery for Novel Therapies Treating Spinal Cord Injury Related Neuropathic Pain

Practical Considerations of Convection Enhanced Delivery for Novel Therapies Treating Spinal Cord Injury Related Neuropathic Pain

miRNAs being Key Contributors to Chronic Pain Development and Maintenance

Traumatic brain injury increases levels of miR‐21 in extracellular vesicles: implications for neuroinflammation

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