Nerve regeneration changes with filters of different pore size

Jenq, Chung Bii; Jenq, Lee Lan; Coggeshall, Richard E.

doi:10.1016/0014-4886(87)90123-3

Cited by 61 publications

(39 citation statements)

References 15 publications

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“…The highly porous structure of the conduit makes it highly permeable, which is essential for the entry of nutrients into the conduit lumen to promote nerve regeneration and at the same time has the necessary barrier to prevent the infiltration of unwanted tissues into the conduit from outside. 35 The developed conduit has no problem of tube breakage that is often encountered with other types of solid polymer conduits. It is easy to fabricate the MNGC by the present microbraiding technique, into any required length and diameter and has no dimensional limitations.…”

Section: Microbraided Nerve Guide Conduitsmentioning

confidence: 99%

Peripheral nerve regeneration by microbraided poly(L‐lactide‐co‐glycolide) biodegradable polymer fibers

Bini

Gao

et al. 2003

J Biomedical Materials Res

155

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Tiny tubes with fiber architecture were developed by a novel method of fabrication upon introducing some modification to the microbraiding technique, to function as nerve guide conduit and the feasibility of in vivo nerve regeneration was investigated through several of these conduits. Poly(L-lactide-co-glycolide) (10:90) polymer fibers being biocompatible and biodegradable were used for the fabrication of the conduits. The microbraided nerve guide conduits (MNGCs) were characterized using scanning electron microscopy to study the surface morphology and fiber arrangement. Degradation tests were performed and the micrographs of the conduit showed that the degradation of the conduit is by fiber breakage indicating bulk hydrolysis of the polymer. Biological performances of the conduits were examined in the rat sciatic nerve model with a 12-mm gap. After implantation of the MNGC to the right sciatic nerve of the rat, there was no inflammatory response. One week after implantation, a thin tissue capsule was formed on the outer surface of the conduit, indicating good biological response of the conduit. Fibrin matrix cable formation was seen inside the MNGC after 1 week implantation. One month after implantation, 9 of 10 rats showed successful nerve regeneration. None of the implanted tubes showed tube breakage. The MNGCs were flexible, permeable, and showed no swelling apart from its other advantages. Thus, these new poly(L-lactide-co-glycolide) microbraided conduits can be effective aids for nerve regeneration and repair and may lead to clinical applications.

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Section: Microbraided Nerve Guide Conduitsmentioning

confidence: 99%

Peripheral nerve regeneration by microbraided poly(L‐lactide‐co‐glycolide) biodegradable polymer fibers

Bini

Gao

et al. 2003

J Biomedical Materials Res

155

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“…A variety of treatments of the nerve gap have been described, including changes to the inner diameter of the nerve guide, 6-8 the addition of saline or dialyzed fibrinogen-containing plasma, 7,9 or alterations to the permeability of the nerve guides. [10][11][12] These treatments, however, likely affected the fibrin matrix micromorphology (fiber bundle diameter and spacing and density, i.e., concentration of fibrin in mg/mL), a variable that has not been studied systematically. Matrix properties such as degradability, porosity, and stiffness are related to fibrin micromorphology and, in principle, could have affected peripheral nerve regeneration.…”

Section: Introductionmentioning

confidence: 98%

Effects of fibrin micromorphology on neurite growth from dorsal root ganglia cultured in three-dimensional fibrin gels

Herbert

Nagaswami

Bittner

et al. 1998

J. Biomed. Mater. Res.

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The effect of fibrin matrix micromorphology on neurite growth was investigated by measuring the length of neurites growing in three-dimensional fibrin gels with well characterized micromorphologies. Dorsal root ganglia (DRGs) from 7-day chick embryos were entrapped and cultured in gels made from varying concentrations of fibrinogen (5-15 mg/mL) or calcium (2-10 mM). The length of growing neurites was measured with light videomicroscopy, and the number and diameter of fibrin fiber bundles were measured from scanning electron micrographs. An increase in fibrinogen concentration caused a decrease in the average fiber bundle thickness, an increase in the number of fiber bundles, and a marked decrease in neurite length. Gels made with different calcium concentrations had a similar range of variation in fibrin fiber bundle number or diameter, but these variations had little effect on neurite and associated nonneuronal cell outgrowth. These results provide insights into the process of neurite advance within fibrin and may be useful in the design of fibrin-based materials used for peripheral nerve regeneration. Furthermore, this study provides the first detailed experimental data on the micromorphology of fibrin matrices made from more than 5 mg/mL of fibrinogen and indicates that existing kinetic models of fibrin polymerization do not accurately predict fibrin structure at these higher concentrations.

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“…El efecto tan marcado del número de agujeros también es compatible con la hipótesis de la fuente externa de fibroblastos. Otros autores han demostrado que si se tapan los agujeros de tubos totalmente permeables con membranas de poros pequeños (0,22 pm), la regeneración se lleva a cabo a una velocidad similar a la que existe en tubos impermeables (14). Estas observaciones también demuestran el papel tan importante de las células externas.…”

Section: Discussionunclassified

Efecto de la permeabilidad en la regeneración del nervio ciático entubado de rata

Hurtado¹

1997

biomedica

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Nerve regeneration changes with filters of different pore size

Cited by 61 publications

References 15 publications

Peripheral nerve regeneration by microbraided poly(L‐lactide‐co‐glycolide) biodegradable polymer fibers

Peripheral nerve regeneration by microbraided poly(L‐lactide‐co‐glycolide) biodegradable polymer fibers

Effects of fibrin micromorphology on neurite growth from dorsal root ganglia cultured in three-dimensional fibrin gels

Efecto de la permeabilidad en la regeneración del nervio ciático entubado de rata

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