Nerve Regeneration in Predegenerated Basal Lamina Graft: The Effect of Duration of Predegeneration on Axonal Extension

Ochi, Mitsuo; Wakasa, Masahiko; Ikuta, Yoshikazu; Kwong, Wing-Hang

doi:10.1006/exnr.1994.1130

Cited by 26 publications

(25 citation statements)

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“…This effect is greatest when HFA is performed with a delay of 10 days. This finding is in line with results from other studies that have shown best implantation results when the future nerve graft is left to predegenerate for 7-14 days (Miyamoto et al, 1985;Ochi et al, 1994;Zhao and Kerns, 1994).…”

Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscsupporting

confidence: 94%

“…It is during this process when the freshly transected hypoglossal nerve is sutured to the degenerating distal nerve segment. In this way, the delayed HFA model is comparable to other modern neurosurgical approaches that report a beneficial influence of predegenerated nerve transplants on axonal regeneration (Kerns et al, 1993;Ochi et al, 1994;Oudega et al, 1994;see below).…”

Section: Delayed Hfa Approachmentioning

confidence: 76%

“…After 4-8 weeks of permanent denervation, the basal lamina tubes begin to fragment (Giannini and Dyck, 1990) and shrink, and the collagen outside the basal laminae thickens (Thomas, 1964). Regeneration of up to 56 days or longer has been shown to be poor in predegenerated grafts (Miyamoto et al, 1985;Ochi et al, 1994). Moreover, after long-term denervation, muscle fibers fail to recover from denervation atrophy (Anzil and Wernig, 1989;Fu and Gordon, 1995b).…”

Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscmentioning

confidence: 98%

“…First, there is active proliferation of nonneuronal cells in the predegenerating distal nerve stump (Ochi et al, 1994), which could cause a maximal breakup of cut-off axons and disintegration of their myelin sheaths during the first 2 weeks after transection (Brown and Hopkins, 1981;Perry et al, 1987), thereby providing more space for the sprouting of the freshly transected hypoglossal axons or axonal branches. Second, the secretion of most neurotrophic factors (e.g., nerve growth factor and its receptor) by the proliferating Schwann cells that follows nerve transection peaks within the first 2 weeks after transection (Stoll et al, 1989;Raivich et al, 1991;Thoenen, 1995).…”

Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscmentioning

confidence: 99%

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Delayed hypoglossal-facial nerve suture after predegeneration of the peripheral facial nerve stump improves the innervation of mimetic musculature by hypoglossal motoneurons

et al. 1997

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Surgical reconstruction of the facial nerve is common clinical practice following destruction of the intracranial facial nerve. Delayed hypoglossal-facial anastomosis (HFA) is the procedure of choice, although the effect of delay on outcome remains unclear. To study the effect of delayed anastomosis on reinnervation, we sutured the proximal stump of a freshly transected hypoglossal nerve of Wistar rats to the distal stump of the ipsilateral facial nerve, which had been transected 7-56 days earlier. Animals that had received HFA without delay served as the control group. Forty days after HFA, horseradish peroxidase (HRP) was injected into the whisker pad; 2 days later, the animals were killed. Reinnervation was assessed by determining the proportion of labeled neuronal cell bodies in the brainstem. The control group had 68% reinnervation of these muscles by hypoglossal neurons and had 32% reinnervation by facial neurons. When the distal facial nerve had been allowed to degenerate for 7 days before HFA, reinnervation of the hypoglossal nerve decreased to 54%, and reinnervation by the facial nerve increased to 46%. However, after a delay of 10-56 days, the hypoglossal fraction increased and stabilized at 77%, and the facial motoneuron fraction decreased to 23%. The presence of new neuromuscular junctions was confirmed by HRP labeling of motor end plates in vivo and by electromyography. We conclude that, under the conditions of hypoglossal-facial crossed nerve suture, the predegeneration of the distal stump of a transected facial nerve enhances the reinnervation of facial muscles by hypoglossal axonal sprouts.

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Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscsupporting

confidence: 94%

Section: Delayed Hfa Approachmentioning

confidence: 76%

Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscmentioning

confidence: 98%

Section: De Novo Innervation Of Predegenerated Peripheral Facial Muscmentioning

confidence: 99%

See 2 more Smart Citations

Delayed hypoglossal-facial nerve suture after predegeneration of the peripheral facial nerve stump improves the innervation of mimetic musculature by hypoglossal motoneurons

et al. 1997

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“…Basement membrane tubes can facilitate regrowth into a nerve graft even when all cellular constituents of the graft are eliminated by repeated freeze-thawing to produce an acellular scaffold (23,48). Acellular grafts, where basement membrane tubes without Schwann cells provide paths for axonal regrowth, can support functional reinnervation over a gap of several centimeters (50) and maintain this potential for months (51).…”

Section: Figurementioning

confidence: 99%

Rapid vascular regrowth in tumors after reversal of VEGF inhibition

Mancuso¹,

Davis²,

Norberg³

et al. 2006

Journal of Clinical Investigation

752

549

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Inhibitors of VEGF signaling can block angiogenesis and reduce tumor vascularity, but little is known about the reversibility of these changes after treatment ends. In the present study, regrowth of blood vessels in spontaneous RIP-Tag2 tumors and implanted Lewis lung carcinomas in mice was assessed after inhibition of VEGF receptor signaling by AG-013736 or AG-028262 for 7 days. Both agents caused loss of 50%-60% of tumor vasculature. Empty sleeves of basement membrane were left behind. Pericytes also survived but had less α-SMA immunoreactivity. One day after drug withdrawal, endothelial sprouts grew into empty sleeves of basement membrane. Vessel patency and connection to the bloodstream followed close behind. By 7 days, tumors were fully revascularized, and the pericyte phenotype returned to baseline. Importantly, the regrown vasculature regressed as much during a second treatment as it did in the first. Inhibition of MMPs or targeting of type IV collagen cryptic sites by antibody HUIV26 did not eliminate the sleeves or slow revascularization. These results suggest that empty sleeves of basement membrane and accompanying pericytes provide a scaffold for rapid revascularization of tumors after removal of anti-VEGF therapy and highlight their importance as potential targets in cancer therapy.

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Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre‐degenerated peripheral nerves

Marcol

Kotulska

et al. 2003

J of Neuroscience Research

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Despite numerous experimental and clinical attempts to reconstruct injuries of peripheral nerves, the methods developed until now have not been sufficiently effective. We examined the influence of extracts (postmicrosomal fractions) obtained from non-pre-degenerated or 7-day-pre-degenerated distal segments of peripheral nerves on the regeneration of injured sciatic nerves of male adult rats. The extracts were introduced to the site of injury with autologous connective tissue chambers filled with fibrin. Reference groups were treated with brain-derived neurotrophic factor (BDNF) or fibrin only. We examined DiI-labeled motoneurons, toluidine blue-labeled myelinated fibers in the mid-part of the chambers, and AChE-positive nerve endings to assess the regeneration intensity. In addition, the length of fibers regrowing within the chambers was measured. We found that extracts obtained from distal stumps of 7-day-pre-degenerated peripheral nerves enhanced nerve regeneration as strongly as BDNF.

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Nerve Regeneration in Predegenerated Basal Lamina Graft: The Effect of Duration of Predegeneration on Axonal Extension

Cited by 26 publications

References 0 publications

Delayed hypoglossal-facial nerve suture after predegeneration of the peripheral facial nerve stump improves the innervation of mimetic musculature by hypoglossal motoneurons

Delayed hypoglossal-facial nerve suture after predegeneration of the peripheral facial nerve stump improves the innervation of mimetic musculature by hypoglossal motoneurons

Rapid vascular regrowth in tumors after reversal of VEGF inhibition

Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre‐degenerated peripheral nerves

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