Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre‐degenerated peripheral nerves

Marcol, Wiesław; Kotulska, Katarzyna; E, Swiech-Sabuda; Larysz‐Brysz, Magdalena; Gołka, Beata; Górka, Dariusz; Lewin–Kowalik, Joanna

doi:10.1002/jnr.10560

Cited by 21 publications

(11 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our previous studies revealed that the extracts obtained from degenerating nerves strongly enhance the morphological features of nerve regeneration in so-called dead-ended autologous connective-tissue chambers. 25 The current results seem to be greatly important from the practical point of view because they are very similar to the results observed by us as well as by many other researches in standard nerve autograft techniques. 4,35,38,40 We also demonstrated, in all groups, that the greatest numbers of AChE-positive nerve fibers were present in the proximal part of the chambers, where bulges could be observed.…”

Section: Discussionsupporting

confidence: 91%

“…9,11,41 An additional supply of the chambers with known neuroactive substances, BDNF and PD fractions, resulted in further regenerative improvement. 25 We chose BDNF for the reference group because it was shown that collagen tubulization and BDNF enhance functional recovery of peripheral nerves, and its beneficial effect on motoneurons is well- documented. 13À15,31,42À47 In postmicrosomal fractions, obtained on day 7 of degeneration, a majority of neurotrophic activity is attributed to increased levels of nerve growth factor (NGF) and not BDNF, because the level of the latter is low on that day and reaches its maximum on day 28 of nerve degeneration.…”

Section: Discussionmentioning

confidence: 99%

“…12 Our previous studies revealed that the extracts obtained from degenerating nerves strongly enhance the morphological features of nerve regeneration in adult rats. 25 The activity of these extracts depends on the period from nerve transection to the nerve collection and preparation of the extract. 26,27 Protein fractions obtained from nerves predegenerated for 7 days proved to exert the strongest neurotrophic effect on both peripheral and central nerve fibers.…”

Section: 25à9mentioning

confidence: 99%

See 2 more Smart Citations

Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

et al. 2005

Self Cite

View full text Add to dashboard Cite

Gap injuries of peripheral nerves, resulting from trauma or neurosurgical procedures, presage badly, for the presence of the distal stump of the nerve seems to be indispensable for regeneration. The standard grafting method requires a lesion of a healthy nerve, and therefore various substitutional materials are under consideration. The aim of the present work was to examine the recovery of rat sciatic nerves after supplying 10-mm-long gaps with an autologous connective-tissue chambers filled with fibrin only or fibrin and various neuroactive substances (brain-derived neurotrophic factor (BDNF), extracts from predegenerated or non-predegenerated nerves). The nerves were allowed to regenerate for 16 weeks. Recovery was measured functionally using the sciatic functional index, and by comparing the weight ratios of calf muscles. The histologic features of regeneration were assessed by counting the number of acetylcholinesterase-positive nerve fibers present inside implanted chambers. We found that chambers filled with fibrin and predegenerated peripheral nerve extracts or BDNF supported functional nerve regeneration much more strongly than chambers filled with fibrin only or fibrin and non-predegenerated peripheral nerve extracts. We conclude that autologous connective-tissue chambers filled with fibrin and predegenerated peripheral nerve extracts or BDNF seem to be a promising tool in peripheral nerve gap injury treatment, with likely clinical implications.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: 25à9mentioning

confidence: 99%

See 1 more Smart Citation

Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…These cytokines and growth factors play important roles during the development and regeneration of peripheral nerves (Chen et al 2000;Chen et al 2001a, b;Lee et al 2003;Marcol et al 2003). Among the factors, neurotrophic factors and extracellular matrix proteins are regarded as growth-promoting factors that stimulate neurite outgrowth of neurons and promote proliferation and migration of SCs (Anton et al 1994).…”

Section: Discussionmentioning

confidence: 98%

Neurotrophic Actions of Bone Marrow Stromal Cells on Primary Culture of Dorsal Root Ganglion Tissues and Neurons

Wang

Ding

et al. 2009

J Mol Neurosci

View full text Add to dashboard Cite

Application of adult bone marrow stromal cells (BMSCs) provides therapeutic benefits to the treatment of neurological insults. The aim of this study was to explore the potential of nonhematopoietic BMSCs to produce soluble factors and stimulate signaling pathways in neurons that mediate trophic effects. A combination of enzyme-linked immunosorbent assay and two-dimensional gel electrophoresis coupled with mass spectrometry showed that the BMSCs released into the culture medium an array of soluble factors such as nerve growth factor, brain-derived neurotrophic factor, basic fibroblast growth factor, and ciliary neurotrophic factor, which have been shown to exhibit potent neurotrophic effects on neural cells. Immunochemistry, cell viability assay, and quantitative real-time RT-PCR collectively showed that neurite outgrowth and neurogenesis in cultured rat dorsal root ganglion (DRG) explants and neurons were enhanced after they were cocultured with rat BMSCs. Western blot analysis revealed that BMSC-conditioned medium activated phosphorylation of mitogen-activated protein kinase/extracellular signal-regulated protein kinase and/or phosphoinositide 3-kinase/serine/threonine kinase (PI3K/Akt) in primary culture of rat DRG neurons, which suggested that BMSCs trigger endogenous survival signaling pathways in neurons through their secreted soluble factors. Our data help to elucidate the mechanisms by which BMSCs function as a cell therapy agent in peripheral nerve regeneration.

show abstract

“…Many investigators equate autotomy behavior with the presence of neuropathic pain in animals (Wall et al, 1979; Coderre et al, 1986; Marcol et al, 2003). The mechanisms of autotomy are not clearly understood, but it is acknowledged that both central and peripheral mechanisms are involved.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of copper/zinc‐superoxide dismutase in transgenic mice markedly impairs regeneration and increases development of neuropathic pain after sciatic nerve injury

Kotulska

LePecheur

Marcol

et al. 2006

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

Despite the general capacity of peripheral nervous system to regenerate, peripheral nerve injury is often followed by incomplete recovery of function, sometimes with the burden of neuropathic pain. The mechanisms of both regeneration and nociception have not been clarified, but it is known that inflammatory reactions are involved. Cu/Zn-superoxide dismutase (SOD1) is an important scavenger protein that acts against oxidative stress. It has been shown to play an important role in apoptosis and inflammation. The aim of this study was to examine the role of SOD1 overexpression in peripheral nerve regeneration and neuropathic pain-related behavior in mice. Sciatic nerves of SOD1-overexpressing and FVB/N wild type-mice were transected and immediately resutured. Evaluation of motor and sensory function and autotomy was carried out during 4 weeks of followup. We found markedly worse sciatic function index outcome as well as more significant atrophy of denervated muscles in SOD1-overexpressing animals compared with wild type. Autotomy was markedly worse in SOD1 transgenic mice than in wild-type animals. Histological evaluation revealed that the intensity of regeneration features, including numbers of GAP-43-positive growth cones, Schwann cells, and macrophages in the distal stump of the transected nerve, was also decreased in transgenic mice. Neuroma formation at the injury site was significantly more prominent in this group. Taken together, our findings suggest that SOD1 overexpression is deleterious for nerve regeneration processes and aggravates neuropathic pain-like state in mice. This can be at least partially ascribed to disturbed inflammatory reactions at the injury site.

show abstract

Regeneration of sciatic nerves of adult rats induced by extracts from distal stumps of pre‐degenerated peripheral nerves

Cited by 21 publications

References 53 publications

Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

Extracts obtained from predegenerated nerves improve functional recovery after sciatic nerve transection

Neurotrophic Actions of Bone Marrow Stromal Cells on Primary Culture of Dorsal Root Ganglion Tissues and Neurons

Overexpression of copper/zinc‐superoxide dismutase in transgenic mice markedly impairs regeneration and increases development of neuropathic pain after sciatic nerve injury

Contact Info

Product

Resources

About