Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture

Bock, Elisabeth; Yavin, Ziva; Jørgensen, Ole Steen; Yavin, Ephraïm

doi:10.1111/j.1471-4159.1980.tb09001.x

Cited by 61 publications

(13 citation statements)

References 26 publications

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“…They describe a monoclonal antibody (anti-BSP 2) recognizing the D2 antigen which labels the surface of different cerebellar cell types at different stages of cerebellar development. Still in cultures of cerebellar interneurones anti-BSP 2 exclusively labels neurones (Hirn et al,198 I) in agreement with the exclusive neuronal localization in brain cell cultures described by others (Bock et al, 1980;Meier et al, 1982). Thus such primary cultures may offer a more simple system for the study of the role of D2 on the neuronal surface membrane and thereby may prove valuable for the elucidation of the functional importance of D2 for neuronal development in vivo.…”

Section: E Meier Et Alsupporting

confidence: 67%

Changes in the Expression of a Neuronal Surface Protein During Development of Cerebellar Neurones In Vivo and in Culture

Meier

Regan

Balázs³

1984

Journal of Neurochemistry

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The expression of the neurone-specific D2 protein changes both quantitatively and qualitatively during development in vivo and in cultures of cerebellar nerve cells. The total D2 content per unit protein shows a two-fold increase in vivo from birth to postnatal day 6, after which it declines progressively to about 50% of the maximal value. This increase can be accounted for by an immature form of the protein anodic D2 being preferentially expressed at the early stages of cerebellar development. After postnatal day 9 this form gradually switches to a mature form cathodic D2. This switch can be mimicked by neuraminidase treatment, suggesting a developmental loss of sialic acid from the D2 protein. In freshly isolated cells the total D2 content per unit protein is only 30% of that in the corresponding intact tissue from 8-day-old cerebella, but it increases rapidly during the first 8 days of culture to levels similar to those of the equivalent age in vivo. The switch from anodic D2 to cathodic D2 also occurs at a faster rate in culture, probably reflecting the culture conditions that favour differentiation. The changes in the expression of D2 during development of cerebellar nerve cells in culture suggest that anodic D2 is preferentially expressed on nerve cells that are proliferating, migrating, or in the initial stages of differentiation, whereas cathodic D2 is associated with differentiated neurones. The transition between the two forms appears to occur during the formation of interneuronal contacts.

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Section: E Meier Et Alsupporting

confidence: 67%

Changes in the Expression of a Neuronal Surface Protein During Development of Cerebellar Neurones In Vivo and in Culture

Meier

Regan

Balázs³

1984

Journal of Neurochemistry

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“…No further change in the average amount of GFAP was observed up to 78 DIV (latest time studied). The concentration of GFAP in these cul tures is similar to that demonstrated in cul tures of dissociated cerebral cells from fetal or newborn rats or mice [4,24,25,29].…”

Section: Immunocytochemistrysupporting

confidence: 48%

Cellular Development and Myelin Production in Primary Cultures of Embryonic Mouse Brain

Fabre¹,

Langley

Bologa

et al. 1985

Dev Neurosci

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The development of cell cultures from embryonic mouse cerebral hemispheres has been followed in detail for periods up to 40 days in culture using a variety of approaches. Functionally well differentiated neurones (shown by receptor binding studies, immunocytochemistry and morphological examination) were found to be abundant early in culture and to form cell contacts with oligodendrocytes characterized both immunocytochemically and morphologically. Myelin-like membranes with the periodicity of classical myelin elaborated by oligodendrocytes were detected only after 30 days in culture when neurones were no longer present. These results are discussed with regard to possible mechanisms of initiation of myelin synthesis.

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“…The second phase was distinguished by a relatively higher content of GM3 and GD3 and of the disialogangliosides GDla and GDlb. These data are in favor of a glial origin, as demonstrated by TEM and by the use of one of the better astrocyte markers, ie, GFAP [Pfeiffer et al, 1977;Bignami et al, 1980;Bock et al, 1980;Stieg et al, 19801, the newly demonstrated oligodendrocyte marker; ie, CAI1 [Vincendon et al, 19781, UDP-ga1actose:ceramide galactosyltransferase cerebroside and monogalactosyl diacylglycerol sulfotransferases [Sarlike et al,198Odl of the cells encountered during that phase.…”

Section: Discussionmentioning

confidence: 99%

Investigations on myelination in vitro. III. Ultrastructural, biochemical, and immunohistochemical studies in cultures of dissociated brain cells from embryonic mice

Sarliève

Delaunoy

Dierich

et al. 1981

J of Neuroscience Research

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Cerebral hemispheres of 14-to 15-day-old mouse embryos were dissociated mechanically into single cells and cultured in polylysine-coated plastic flasks. The succession of changes and the identification of cell populations have been studied by transmission electron microscopy (TEM). Four distinct cell types can be clearly defined. Neuroblasts, oligodendroblasts, and astroblasts were used to denote embryonic states of neurons, oligodendrocytes, and astrocytes. Cell populations were: 1) neuroblasts; 2) astroblasts, either growing on the surface of the flasks and forming a basal layer of epithelioid cells or scattered at the surface of the bed layer; 3) oligodendroblasts, characterized by a high nucleocytoplasmic ratio and resting on top of the bed layer; 4) macrophages. Incorporation of [3H]thymidine into cells at different days in culture (DIC) delineated at least three periods of DNA synthesis (from 1 to 6, from 10 to 20, and from 25 to 60 DIC). High cell RNA/DNA and protein/DNA ratios indicated an increase in the cytoplasmic mass. Guanylate cyclase (EC 4.6.1.2; GC) and adenylate cyclase (EC 4.6.1 . l ; AC) activities and the ratio AC/GC also agreed with the existence of different periods in culture, but the yin yang hypothesis (postulated by Goldberg et al, 1975) was only partially confirmed. Concerning membrane constituents, the evolution of the pattern of the major gangliosides showed a phase from 1 t o 20 DIC which was characterized during the first half of this period by a high percentage of tri-and tetra-sialogangliosides, thereby confirming the Abbreviations: AC, adenylate cyclase; AChE, acetylcholinesterase; Anti-CAll, anti-carbonic anhydrase isoenzyme 11; Anti-GFAP, anti-glial fibrillary acidic protein; ChAC, choline acetyltransferase; DIC, days in culture; GC, guanylate cyclase; SEM, scanning electron microscopy; TEM, transmission electron microscopy; TLC, thin-layer chromatography.

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Nervous System‐Specific Proteins in Developing Rat Cerebral Cells in Culture

Cited by 61 publications

References 26 publications

Changes in the Expression of a Neuronal Surface Protein During Development of Cerebellar Neurones In Vivo and in Culture

Changes in the Expression of a Neuronal Surface Protein During Development of Cerebellar Neurones In Vivo and in Culture

Cellular Development and Myelin Production in Primary Cultures of Embryonic Mouse Brain

Investigations on myelination in vitro. III. Ultrastructural, biochemical, and immunohistochemical studies in cultures of dissociated brain cells from embryonic mice

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