Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human glioma cell line U87MG

Yasmin, Ishmat Ara; Sundaram, Sumati; Banerjee, Anasuya; Varier, Lavanya; Dharmarajan, Arun; Warrier, Sudha

doi:10.1016/j.yexcr.2021.112912

Cited by 9 publications

(11 citation statements)

References 79 publications

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“…Decorin (DCN) is an important antitumor component of extracellular matrix [ 42 ], produced by fibroblasts, whose interaction with EGFR is well studied, moreover, decorin acts as a pan-receptor inhibitor of tyrosine kinases, binding also to HER2, HGFR/Met, VEGFR2, TLR and IGFR [ 43 ]. SFRP4 is a secreted protein associated with some aggressive cancers, in case of glioblastoma its action can be proapoptotic [ 44 ], it is probably an oncosuppressor, and its mutations reverse this function, which is especially evident in ovarian cancer [ 45 , 46 ].…”

Section: Resultsmentioning

confidence: 99%

Interactome of Paraoxonase PON2 Reveals New Pathways for Tumor Growth Regulation

Karlov

Pestov²,

Shakhparonov³

et al. 2023

Dokl Biochem Biophys

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The interactome of paraoxonase-2 encoded by the PON2 gene was investigated. A cDNA library was screened using a yeast two-hybrid system to search for new proteins interacting with human PON2. Analysis of the identified candidates, along with previously published data on interactors obtained by other methods, indicates the presence of a significant number of indirect interactions between PON2 and EGFR and, consequently, possible regulation of tumor growth with mutant EGFR involving PON2.

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Section: Resultsmentioning

confidence: 99%

Interactome of Paraoxonase PON2 Reveals New Pathways for Tumor Growth Regulation

Karlov

Pestov²,

Shakhparonov³

et al. 2023

Dokl Biochem Biophys

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“…Various studies have shown that SFRP4 has the ability to modulate a variety of biological processes. SFRP4 has been demonstrated in several studies to have dual functions, not only increasing apoptosis and negatively regulating differentiation ( 41 – 43 ) but also modulating cardiomyocyte ( 44 ) and skin keratinocyte differentiation in a targeted way ( 45 ). IF confirmed that SFRP4 + NKT cells were present in the lesions of patients with adenomyosis pain and produced a significant quantity of SFRP4 into the extracellular tissue space.…”

Section: Discussionmentioning

confidence: 99%

SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

Chen

Zhu²,

Chen³

et al. 2022

Front. Immunol.

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BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain.

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“…It also contains a netrinlike (NTR) domain on the C-terminal, possessing the ability to suppress matrix metalloproteinases [23] . Studies revealed that many proteins contain the NTR domain, among which, secreted frizzled-related proteins act in synergy with PCPE1 to enhance the cleavage activity of BMP1 [24][25] . Therefore, PCPE1 has pleiotropic effects in promoting collagen deposition.…”

Section: Discussionmentioning

confidence: 99%

Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen

Luo¹,

Chen²,

Liu³

et al. 2022

Int J Ophthalmol

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AIM: To investigate the role of procollagen C-proteinase enhancer 1 (PCPE1) in retinal angiogenesis and relevant mechanisms. METHODS: The Pcolce1-knockout (KO) mice were used to explore the effect of PCPE1 on retinal angiogenesis in vivo. Pcolce1 siRNA were designed, cell count kit 8 (CCK8) assays and tube formation assays were performed to investigate the cell proliferation and tube formation abilities of retinal microvascular endothelial cells (hRMECs) in vitro. Mouse embryo fibroblasts (MEF) cells were isolated and cultured to analyze the effect of PCPE1 on enhancing procollagen cleavage. RESULTS: In vivo studies showed that the retinal vascular density of Pcolce1-/- mice was significantly lower than that of the control group. Furthermore, silencing of Pcolce1 inhibited cell proliferation and tube formation abilities of hRMECs in vitro. Additionally, much more pro-collagen was found in Pcolce1-/- MEF cells, compared to wild type MEF cells. CONCLUSION: PCPE1 may promote physiological retinal angiogenesis by regulating the processing of collagen, which may provide a potential therapeutic target of retinal vascular disease.

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Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human glioma cell line U87MG

Cited by 9 publications

References 79 publications

Interactome of Paraoxonase PON2 Reveals New Pathways for Tumor Growth Regulation

Interactome of Paraoxonase PON2 Reveals New Pathways for Tumor Growth Regulation

SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

Procollagen C-proteinase enhancer 1 promotes physiologic retinal angiogenesis via regulating the process of collagen

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