Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia

Xiangli,; Bo-Xing,; Xin-Liu,; Jiang, Xiaowen; Lu, Hsu Feng; Xu, Zihua; Yue-Yang,; Qiong-Wu,; Dong-Yao,; Zhang, Yingshi

doi:10.1016/j.fitote.2020.104824

Cited by 15 publications

(3 citation statements)

References 77 publications

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“…For example, gene AT3G03050 in Arabidopsis thaliana, which is homologous to Soltu.DM.03G020780, is involved in the plant cell wall and root hairs and plays a central role in plant growth, development, and stress response [26]. The gene AT4G25480 in Arabidopsis thaliana, which is homologous to Soltu.DM.03G016740, belongs to the ERF/AP2 family of transcription factors and responds to stress and ABA [27]. The gene AT1G27730 in Arabidopsis thaliana, which is homologous to Soltu.DM.01G046560, responds to salt stress and oxidative stress [28].…”

Section: Visualization Of the Core Genes In Candidate Modules In Gene...mentioning

confidence: 99%

Comprehensive Transcriptome and Proteome Analyses Reveal the Drought Responsive Gene Network in Potato Roots

Qin,

Wang,

et al. 2024

Plants

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The root system plays a decisive role in the growth and development of plants. The water requirement of a root system depends strongly on the plant species. Potatoes are an important food and vegetable crop grown worldwide, especially under irrigation in arid and semi-arid regions. However, the expected impact of global warming on potato yields calls for an investigation of genes related to root development and drought resistance signaling pathways in potatoes. In this study, we investigated the molecular mechanisms of different drought-tolerant potato root systems in response to drought stress under controlled water conditions, using potato as a model. We analyzed the transcriptome and proteome of the drought-sensitive potato cultivar Atlantic (Atl) and the drought-tolerant cultivar Qingshu 9 (Q9) under normal irrigation (CK) and weekly drought stress (D). The results showed that a total of 14,113 differentially expressed genes (DEGs) and 5596 differentially expressed proteins (DEPs) were identified in the cultivars. A heat map analysis of DEGs and DEPs showed that the same genes and proteins in Atl and Q9 exhibited different expression patterns under drought stress. Weighted gene correlation network analysis (WGCNA) showed that in Atl, Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG)-enriched pathways were related to pyruvate metabolism and glycolysis, as well as cellular signaling and ion transmembrane transporter protein activity. However, GO terms and KEGG-enriched pathways related to phytohormone signaling and the tricarboxylic acid cycle were predominantly enriched in Q9. The present study provides a unique genetic resource to effectively explore the functional genes and uncover the molecular regulatory mechanism of the potato root system in response to drought stress.

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Section: Visualization Of the Core Genes In Candidate Modules In Gene...mentioning

confidence: 99%

Comprehensive Transcriptome and Proteome Analyses Reveal the Drought Responsive Gene Network in Potato Roots

Qin,

Wang,

et al. 2024

Plants

View full text Add to dashboard Cite

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“…The common targets were imported into the OmicShare tools https://www.omicsshare.com/ for Gene Ontology enrichment(GO) and Kyoto Encyclopedia of Genes and Genomes pathway analysis (KEGG) ( Xiang L et al 2021). The enrichment results of GO function and KEGG pathway were obtained by setting p-value.…”

Section: Go and Kegg Pathway Enrichment Analysismentioning

confidence: 99%

Study The Mechanism of Huangdi Anxiao Capsules In The Treatment of T2DM of Using UPLC-Q-TOF-MSE Combined With Network Pharmacological

Zhang

Zhu

et al. 2022

Preprint

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This study was to explore the main material basis, target and pathway of Huangdi Anxiao capsule (HDAXC) for the treatment of type 2 diabetes mellitus (T2DM) by UPLC-Q-TOF-MSE and network pharmacology. In this study, HDAXC was administrated to T2DM rats, and its serum were detected by UPLC- Q-TOF-MSE, and the prototype components of HDAXC were analyzed. Using Swiss target prediction database to predict the target of serum prototype components, using GeneCards and DrugBack database to predict the target of T2DM. These common targets are the prediction target of HDAXC acting on T2DM. The key components of HDAXC in the treatment of T2DM were determined by using the software of Cytoscape3.7.2 to visualize the results. Using the STRING online platform to construct the protein-protein interaction (PPI), the key target genes were selected. The Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the common targets were carried out by using the OmicShare tools. And quantitative PCR and Western bolt were used to verify the related target genes. A toll of 28 prototype compounds were detected in rat serum, and 495 putative identified target genes were screened from HDAXC, of which 141 overlapped with the targets of T2DM and were considered potential therapeutic targets. The analysis of the network results showed that the key components of HDAXC are Magnoflorine, Galangin, Quercetin, and Epiberberine, etc. VEGFA, AKT1, SRC, EGFR might be the key target genes of HDAXC in the treatment of T2DM. HDAXC may have a therapeutic effect on T2DM by affecting HIF-1 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, VEGFA signaling pathway and PI3K/AKT signaling pathways. In this study, compounds absorbed into the blood of HDAXC and its action target and pathway were preliminarily analyzed, which provided evidences for clarifying the chemical material basis and researching functional mechanism of HDAXC.

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“…org/, version 11.0). The protein's topology analysis was executed by Cytoscape 3.7.2 with the plugin tool "CytoNCA" (Li et al, 2021).…”

Section: Network Constructionmentioning

confidence: 99%

Kun-Dan Decoction Ameliorates Insulin Resistance by Activating AMPK/mTOR-Mediated Autophagy in High-Fat Diet-Fed Rats

Zeng

Feng

et al. 2021

Front. Pharmacol.

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Background: Metabolic syndrome is characterized by central obesity, hyperglycemia and hyperlipidemia. Insulin resistance is the leading risk factor for metabolic syndrome. Kun-Dan decoction (KD), a traditional Chinese medicine, has been applied to treat patients with metabolic syndrome for over ten years. It is increasingly recognized that autophagy deficiency is the key cause of metabolic syndrome. Therefore, we aimed to explore whether KD can activate autophagy to improve metabolic syndrome.Methods: Network pharmacology was used to explore the underlying mechanism of KD in the treatment of metabolic syndrome. The high-fat diet-fed rats and oleic acid-induced LO2 cells were employed in our study. Oral glucose tolerance test and insulin tolerance test, obesity and histological examination, serum cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), homeostasis model assessment of insulin resistance (HOMA-IR) and insulin sensitivity in high-fat diet-fed rats were analyzed. Furthermore, the protein expressions of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK), phospho-AMPK, mammalian target of rapamycin (mTOR), phospho-mTOR, p62, autophagy related protein (Atg) 5, Atg7, Atg12, Atg13, Atg16L1 and microtubule-associated protein 1A/1B-light chain 3 (LC3)-Ⅱ/Ⅰ were examined in rats and LO2 cells. Moreover, autophagy activator rapamycin and inhibitor 3-methyladenine, and small interfering RNA against Atg7 were utilized to verify the role of autophagy in the treatment of metabolic syndrome by KD in oleic acid-induced LO2 cells.Results: Results from network pharmacology indicated that targeted insulin resistance might be the critical mechanism of KD in the treatment of metabolic syndrome. We found that KD significantly suppressed obesity, serum cholesterol, triglyceride and LDL-C levels and increased serum HDL-C level in high-fat diet-fed rats. Furthermore, KD enhanced insulin sensitivity and attenuated HOMA-IR in high-fat diet-fed rats. Western blot showed that KD could enhance autophagy to increase the insulin sensitivity of high-fat diet-fed rats and oleic acid-induced LO2 cells. Furthermore, 3-methyladenine and small interfering RNA against Atg7 could reverse the protective effect of KD on LO2 cells. However, rapamycin could cooperate with KD to enhance autophagic activation to increase insulin sensitivity in LO2 cells.Conclusion: The induction of autophagy may be the major mechanism for KD to improve insulin resistance and metabolic syndrome.

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Network pharmacology-based research uncovers cold resistance and thermogenesis mechanism of Cinnamomum cassia

Cited by 15 publications

References 77 publications

Comprehensive Transcriptome and Proteome Analyses Reveal the Drought Responsive Gene Network in Potato Roots

Comprehensive Transcriptome and Proteome Analyses Reveal the Drought Responsive Gene Network in Potato Roots

Study The Mechanism of Huangdi Anxiao Capsules In The Treatment of T2DM of Using UPLC-Q-TOF-MSE Combined With Network Pharmacological

Kun-Dan Decoction Ameliorates Insulin Resistance by Activating AMPK/mTOR-Mediated Autophagy in High-Fat Diet-Fed Rats

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