Network pharmacology combined with metabolomics and lipidomics to reveal the hypolipidemic mechanism of<i>Alismatis rhizoma</i>in hyperlipidemic mice

Pan, Yan; Wei, Yinyu; Wang, Meiqin; Tao, Jianmei; Ouyang, Hui; Du, Zhifeng; Li, Sen; Jiang, Hongliang

doi:10.1039/d1fo04386b

Cited by 37 publications

(18 citation statements)

References 75 publications

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“…HZ treatment reversed these glycerophospholipids toward normal levels. Our findings are partly consistent with previous reports that HFD-induced disorders in the body are often accompanied by abnormal variation in glycerophospholipids, including a higher level of LysoPC(18:1) [36] and a lower level of LysoPC(16: 0) [37]. Therefore, the alteration in glycerophospholipids was a common feature of high-fat diet-induced hyperlipidemia and was associated with the hypolipidemic activity of HZ.…”

Section: Glycerophospholipidssupporting

confidence: 92%

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

Yang

et al. 2023

J of Separation Science

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Polygonum cuspidatum is a homology of traditional medicine and functional food widely distributed around the world. Our previous study on the hyperlipidemic animal model demonstrated that Polygonum cuspidatum was effective in ameliorating hyperlipidemia, which is characterized by lipid disorders. Herein, the regulatory effect of Polygonum cuspidatum on lipid metabolism needs to be known if its hypolipidemic mechanism is desired to clarify. In this study, an ultra-high performance liquid chromatography-quadrupole/time-of-flight mass spectrometry-based lipidomic strategy was first applied to investigate the lipidomic patterns of high-fat diet-induced hyperlipidemic hamsters when treated with Polygonum cuspidatum. The results showed that Polygonum cuspidatum improved the lipidomic profile of hyperlipidemia. A total of 65 differential lipids related to the hypolipidemic effect of Polygonum cuspidatum were screened out and identified, and these differential lipids covered various categories, such as phosphatidylcholines, phosphatidylethanolamines, triacylglycerols, sphingomyelins and so on. Orally administrated Polygonum cuspidatum restored these differential lipids back to normal or nearly normal levels. This study adopted lipidomics to reveal the key lipid molecules as potential therapeutic targets of

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Section: Glycerophospholipidssupporting

confidence: 92%

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

Yang

et al. 2023

J of Separation Science

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“…Meanwhile, studies have also shown that ALB is the most important carrier/transporter protein in vivo and plays an essential role in plasma antioxidant activity. In addition, in the HLP model, the decreased levels of ALB may be closely associated with the decline of liver function leading to reduced liver production 50 , 51 . Therefore, we speculate that ALB is mainly used as a drug transport platform to treat HLP by YLTZC.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the mechanisms of Yinlan Tiaozhi capsule in treating hyperlipidemia by combining network pharmacology, molecular docking and experimental verification

Xiao¹,

Hu²,

Jia

et al. 2023

Sci Rep

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Yinlan Tiaozhi capsule (YLTZC) has been widely used to treat hyperlipidemia (HLP). However, its material basis and underlying pharmacological effects remain unclean. The current study aimed to explore the mechanisms involved in the treatment of YLTZC on HLP based on network pharmacology, molecular docking, and experimental verification. Firstly, UPLC-Q-TOF–MS/MS was used to comprehensively analyze and identify the chemical constituents in YLTZC. A total of 66 compounds, mainly including flavonoids, saponins, coumarins, lactones, organic acids, and limonin were characterized and classified. Simultaneously, the mass fragmentation pattern of different types of representative compounds was further explored. By network pharmacology analysis, naringenin and ferulic acid may be the core constituents. The 52 potential targets of YLTZC, including ALB, IL-6, TNF, and VEGFA, were considered potential therapeutic targets. Molecular docking results showed that the core active constituents of YLTZC (naringenin and ferulic acid) have a strong affinity with the core targets of HLP. Lastly, animal experiments confirmed that naringenin and ferulic acid significantly upregulated the mRNA expression of ALB and downregulated the mRNA expression of IL-6, TNF, and VEGFA. In sum, the constituents of YLTZC, such as naringenin and ferulic acid, might treat HLP by regulating the mechanism of angiogenesis and inhibiting inflammatory responses. Furthermore, our data fills the gap in the material basis of YLTZC.

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“…Altogether, these different products, targets, and mechanisms contribute to the efficacy of AR to reduce hyperlipidemia and to combat atherosclerosis [ 73 ]. A recent network analysis pointed out the implications of 6 key targets regulated by AR (albumin, TNF, IL-1β, MMP9, and PPARα/γ) [ 74 ] whereas another study underlined the contribution of the FKBP/mTOR/SREBPs pathway and the direct binding of the active terpenoids to FK506-binding protein FKBP38 [ 10 ]. At least 9 triterpenes are considered to play a significant role in the lipid-lowering effect of AR (out of 87 triterpenes identified in the study) [ 75 ].…”

Section: Pharmacological Properties Of Alisolsmentioning

confidence: 99%

Pharmacological Properties and Molecular Targets of Alisol Triterpenoids from Alismatis Rhizoma

Bailly

2022

Biomedicines

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More than 100 protostane triterpenoids have been isolated from the dried rhizomes of Alisma species, designated Alismatis rhizoma (AR), commonly used in Asian traditional medicine to treat inflammatory and vascular diseases. The main products are the alisols, with the lead compounds alisol-A/-B and their acetate derivatives being the most abundant products in the plant and the best-known bioactive products. The pharmacological effects of Ali-A, Ali-A 24-acetate, Ali-B, Ali-B 23-acetate, and derivatives have been analyzed to provide an overview of the medicinal properties, signaling pathways, and molecular targets at the origin of those activities. Diverse protein targets have been proposed for these natural products, including the farnesoid X receptor, soluble epoxide hydrolase, and other enzymes (AMPK, HCE-2) and functional proteins (YAP, LXR) at the origin of the anti-atherosclerosis, anti-inflammatory, antioxidant, anti-fibrotic, and anti-proliferative activities. Activities were classified in two groups. The lipid-lowering and anti-atherosclerosis effects benefit from robust in vitro and in vivo data (group 1). The anticancer effects of alisols have been largely reported, but, essentially, studies using tumor cell lines and solid in vivo data are lacking (group 2). The survey shed light on the pharmacological properties of alisol triterpenoids frequently found in traditional phytomedicines.

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Network pharmacology combined with metabolomics and lipidomics to reveal the hypolipidemic mechanism ofAlismatis rhizomain hyperlipidemic mice

Abstract: Alismatis Rhizoma (AR), the dried rhizome of Alisma orientale (Sam) Juzep, is effective in treating hyperlipidemia, but the mechanisms involved require further exploration. This study evaluated the hypolipidemic properties of...

Cited by 37 publications

References 75 publications

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

Ultra‐high performance liquid chromatography‐quadrupole/time‐of‐flight mass spectrometry‐based lipidomics reveals key lipid molecules as potential therapeutic targets of Polygonum cuspidatum against hyperlipidemia in a hamster model

Deciphering the mechanisms of Yinlan Tiaozhi capsule in treating hyperlipidemia by combining network pharmacology, molecular docking and experimental verification

Pharmacological Properties and Molecular Targets of Alisol Triterpenoids from Alismatis Rhizoma

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