Neural activity and branching of embryonic retinal ganglion cell dendrites

Hocking, Jennifer C.; Pollock, Natashka S.; Johnston, Jillian; Wilson, Richard J. A.; Shankar, Anoop; McFarlane, Sarah

doi:10.1016/j.mod.2012.05.003

Cited by 8 publications

(7 citation statements)

References 61 publications

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“…Here, we aimed to test our previous hypothesis that evoked calcium firing indicated axonal regeneration. To this end, we regulated the calcium responses as well as neural activity via Kir2.1a overexpression (41,(49)(50)(51)(52)(53), PTZ treatment, and scn1a gene knockout (53,54,56). We observed that these effects on regeneration were correlated with neural activity (Figs.…”

Section: Discussionmentioning

confidence: 95%

“…To further test our hypothesis that the evoked calcium response indicated intrinsic regenerative ability and study the effect of neural activity on axonal regeneration, we overexpressed Kir2.1a, an inward rectifying potassium channel protein that down-regulates neuronal activity (44,(50)(51)(52), in zebrafish M cells using single-cell electroporation at 4 dpf. We expected that the overexpression of Kir2.1a would influence calcium dynamics by down-regulating neuronal activity.…”

Section: Kir21a Inhibited Neural Activity and Axonal Regenerationmentioning

confidence: 99%

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In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

et al. 2019

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Calcium is an important messenger in the neuronal system, but its specific role in axonal regeneration has not been fully investigated. To clarify it, we constructed a noninvasive in vivo calcium‐imaging model of zebrafish Mauthner cells and monitored subcellular calcium dynamics during axonal regeneration. Using the calcium indicator GCamp6f, we observed that the regenerative length correlated with the peak amplitude of the evoked calcium response before axotomy, which suggested that the evoked calcium response might serve as a useful indicator of evoked neuronal activity and axonal regenerative capacity. To investigate this possibility, we overexpressed an inward rectifying potassium channel protein, Kir2.1a, to decrease the Mauthner neuronal activity and found that the inhibition of the calcium response correlated with decreased axonal regeneration. In contrast, treatment of pentylenetetrazol and knockout of the sodium voltage‐gated channel ol subunit 1 gene increased the calcium response and thus enhanced axonal regeneration. Our results therefore increased the understanding of the correlation between the neural activity and the vertebrate axonal regeneration.—Chen, M., Huang, R.‐C, Yang, L.‐Q., Ren, D.‐L., Hu, B. In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish. FASEB J. 33, 7721–7733 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 95%

Section: Kir21a Inhibited Neural Activity and Axonal Regenerationmentioning

confidence: 99%

In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

et al. 2019

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“…Various subtypes of K ir channels have been confirmed to be expressed in RGCs in Xenopus laevis (15) and rats (16,17,22). Specific subunits of K v channels have been observed to be expressed in RGCs in Xenopus laevis (6,15,24,25), goldfish (26), trout (27), mice (28)(29)(30)31), rats (20,32,33) and cats (34). Eag1 and Eag2 have been reported to be expressed in RGCs in rats (21) and cattle (35).…”

Section: Expression Of K + Channels In Rgcsmentioning

confidence: 95%

Section: Expression Of K + Channels In Rgcsmentioning

confidence: 99%

“…Biochemical, molecular and pharmacological studies have identified numerous types of K + channels in RGCs, including K ir (15)(16)(17), K ATP (18), TASK (19), K v (6,15,20), Eag (21) and K Ca channels (Table I) (22,23). Various subtypes of K ir channels have been confirmed to be expressed in RGCs in Xenopus laevis (15) and rats (16,17,22). Specific subunits of K v channels have been observed to be expressed in RGCs in Xenopus laevis (6,15,24,25), goldfish (26), trout (27), mice (28)(29)(30)31), rats (20,32,33) and cats (34).…”

Section: Expression Of K + Channels In Rgcsmentioning

confidence: 99%

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Potassium ion channels in retinal ganglion cells (Review)

Zhong

Wang

Wang-min

et al. 2013

Molecular Medicine Reports

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Retinal ganglion cells (RGCs) consolidate visual processing and constitute the last step prior to the transmission of signals to higher brain centers. RGC death is a major cause of visual impairment in optic neuropathies, including glaucoma, age‑related macular degeneration, diabetic retinopathy, uveoretinitis and vitreoretinopathy. Discharge patterns of RGCs are primarily determined by the presence of ion channels. As the most diverse group of ion channels, potassium (K+) channels play key roles in modulating the electrical properties of RGCs. Biochemical, molecular and pharmacological studies have identified a number of K+ channels in RGCs, including inwardly rectifying K+ (Kir), ATP‑sensitive K+ (KATP), tandem‑pore domain K+ (TASK), voltage‑gated K+ (Kv), ether‑à‑go‑go (Eag) and Ca2+‑activated K+ (KCa) channels. Kir channels are important in the maintenance of the resting membrane potential and controlling RGC excitability. KATP channels are involved in RGC survival and neuroprotection. TASK channels are hypothesized to contribute to the regulation of resting membrane potentials and firing patterns of RGCs. Kv channels are important regulators of cellular excitability, functioning to modulate the amplitude, duration and frequency of action potentials and subthreshold depolarizations, and are also important in RGC development and protection. Eag channels may contribute to dendritic repolarization during excitatory postsynaptic potentials and to the attenuation of the back propagation of action potentials. KCa channels have been observed to contribute to repetitive firing in RGCs. Considering these important roles of K+ channels in RGCs, the study of K+ channels may be beneficial in elucidating the pathophysiology of RGCs and exploring novel RGC protection strategies.

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N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo

Chen

Huang

et al. 2016

Mol Neurobiol

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Stimulating neuronal activity increases myelin sheath formation by individual oligodendrocytes, but how myelination is regulated by neuronal activity in vivo is still not fully understood. While in vitro studies have revealed the important role of N-cadherin in myelination, our understanding in vivo remains quite limited. To obtain the role of N-cadherin during activity-dependent regulation of myelinating capacity of individual oligodendrocytes, we successfully built an in vivo dynamic imaging model of the Mauthner cell at the subcellular structure level in the zebrafish central nervous system. Enhanced green fluorescent protein (EGFP)-tagged N-cadherin was used to visualize the stable accumulations and mobile transports of N-cadherin by single-cell electroporation at the single-cell level. We found that pentylenetetrazol (PTZ) significantly enhanced the accumulation of N-cadherin in Mauthner axons, a response that was paralleled by enhanced sheath number per oligodendrocytes. By offsetting this phenotype using oligopeptide (AHAVD) which blocks the function of N-cadherin, we showed that PTZ regulates myelination in an N-cadherin-dependent manner. What is more, we further suggested that PTZ influences N-cadherin and myelination via a cAMP pathway. Consequently, our data indicated that N-cadherin is involved in neuronal activity-dependent regulation of myelinating capacity of zebrafish individual oligodendrocytes in vivo.

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Neural activity and branching of embryonic retinal ganglion cell dendrites

Cited by 8 publications

References 61 publications

In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

Potassium ion channels in retinal ganglion cells (Review)

N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo

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