Neural differentiation of umbilical cord mesenchymal stem cells by sub-sonic vibration

Cho, Hyunjin; Seo, Young-Kwon; Jeon, Songhee; Yoon, Hee-Hoon; Choi, Yun-Kyong; Park, Jung-Keug

doi:10.1016/j.lfs.2012.02.014

Cited by 35 publications

(22 citation statements)

References 51 publications

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“…There have also been reports that thermal vibratory stimuli can induce stem cell differentiation: Tong et al ( 2013 ) reported that high frequency vibratory stimulation of induced differentiation of multipotent human mesenchymal stem cells in culture. A similar report in the case of human umbilical stem cells was published by Cho et al ( 2012 ). Kim et al ( 2012 ) confirm that human mesenchymal stem cells are mechanosensitive to low-magnitude-high-frequency vibration signals such that they could facilitate the osteogenic process.…”

Section: A Protein Vibration Codesupporting

confidence: 73%

On the possible role of protein vibrations in information processing in the brain: three Russian dolls

Smythies

2015

Front. Mol. Neurosci.

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Until recently it was held that the neurocomputations conducted by the brain involved only whole neurons as the operating units. This may however represent only a part of the mechanism. This theoretical and academic position article reviews the considerable evidence that allosteric interactions between proteins (as extensively described by Fuxe et al., 2014), and in particular protein vibrations in neurons, form small scale codes that are involved as parts of the complex information processing systems of the brain. The argument is then developed to suggest that the protein allosteric and vibration codes (that operate at the molecular level) are nested within a medium scale coding system whose computational units are organelles (such as microtubules). This medium scale code is nested in turn inside a large scale coding system, whose computational units are individual neurons. The hypothesis suggests that these three levels interact vertically in both directions thus materially increasing the computational capacity of the brain. The whole hierarchy is thus similar to three nested Russian dolls. This theoretical development may be of use in the design of experiments to test it.

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Section: A Protein Vibration Codesupporting

confidence: 73%

On the possible role of protein vibrations in information processing in the brain: three Russian dolls

Smythies

2015

Front. Mol. Neurosci.

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“…Compared with bone marrow MSCs, hUCMSCs are easier to isolate and expand, and so they would be an advantageous, novel source of adult MSCs. A number of studies have demonstrated that hUCMSCs may have an important role in the application and experimental research of adult human MSCs ( 8 , 9 ).…”

Section: Discussionmentioning

confidence: 99%

Karyotype stability of human umbilical cord-derived mesenchymal stem cells during in vitro culture

Ruan

Zhu

Yin

et al. 2014

Experimental and Therapeutic Medicine

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The aim of this study was to investigate whether the chromosomes of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) change following in vitro culture for several generations. In the present study, umbilical cords from two healthy infants following cesarean delivery were collected aseptically and hUCMSCs were isolated by digestion with collagenase and trypsin, and then cultured in vitro. hUCMSCs with fibroblastic morphology were presented from the human umbilical cord tissue after 7 days of adherent culture. When cultured for 6 passages in vitro, the hUCMSCs maintained a stable spindle-shaped morphology. Cells reached the logarithmic growth phase after 3–4 days of culture. In addition, CD13, CD29, CD44, CD90 and CD105 were highly expressed in generations P3-P6. The expression of CD31, CD34, CD45 and HLA-DR was negative. Furthermore, karyotype analysis revealed a normal diploid karyotype with 46 chromosomes and no abnormal changes were found in chromosome structure. These findings suggest that when cultured for 6 passages in vitro, hUCMSCs maintain a stable immunophenotype and chromosome structure, which provides an experimental basis for the safety of hUCMSC cytotherapy.

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“…Investigating the potential of UC-derived MSC to differentiate into cells of the ectodermal lineage several groups reported differentiation into neural-like cells [31,32,33,35,36,65,68,91,104,125,126,127,128]. Upon stimulation with potent agents like b-FGF, or retionic acid morphological changes and neuro-specific marker expression, like nestin, β-tubulin III, or neurofilament M, could be observed on the mRNA as well as protein level.…”

Section: Differentiation Potential Of Uc-mscmentioning

confidence: 99%

Mesenchymal Stem or Stromal Cells from Amnion and Umbilical Cord Tissue and Their Potential for Clinical Applications

Lindenmair

Hatlapatka

Kollwig

et al. 2012

Cells

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Mesenchymal stem or stromal cells (MSC) have proven to offer great promise for cell-based therapies and tissue engineering applications, as these cells are capable of extensive self-renewal and display a multilineage differentiation potential. Furthermore, MSC were shown to exhibit immunomodulatory properties and display supportive functions through parakrine effects. Besides bone marrow (BM), still today the most common source of MSC, these cells were found to be present in a variety of postnatal and extraembryonic tissues and organs as well as in a large variety of fetal tissues. Over the last decade, the human umbilical cord and human amnion have been found to be a rich and valuable source of MSC that is bio-equivalent to BM-MSC. Since these tissues are discarded after birth, the cells are easily accessible without ethical concerns.

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Neural differentiation of umbilical cord mesenchymal stem cells by sub-sonic vibration

Cited by 35 publications

References 51 publications

On the possible role of protein vibrations in information processing in the brain: three Russian dolls

On the possible role of protein vibrations in information processing in the brain: three Russian dolls

Karyotype stability of human umbilical cord-derived mesenchymal stem cells during in vitro culture

Mesenchymal Stem or Stromal Cells from Amnion and Umbilical Cord Tissue and Their Potential for Clinical Applications

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