Neural microvascular pericytes contribute to human adult neurogenesis

Farahani, Ramin Hamidi; Rezaei-Lotfi, Saba; Simonian, Mary; Xaymardan, Munira; Hunter, Neil

doi:10.1002/cne.24565

Cited by 30 publications

(36 citation statements)

References 67 publications

(88 reference statements)

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“…A key signature of human neural progenitor cells [30] cultured in 2D monolayers is partial synchronicity of the entry into mitosis at population level [7]. The synchronicity manifests as periods of enhanced mitotic events (Fig.…”

Section: Molecular Basis Of Coupled Cyclingmentioning

confidence: 99%

Coupled cycling and regulation of metazoan morphogenesis

Rezaei-Lotfi

Farahani

2020

Cell Div

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Metazoan animals are characterized by restricted phenotypic heterogeneity (i.e. morphological disparity) of organisms within various species, a feature that contrasts sharply with intra-species morphological diversity observed in the plant kingdom. Robust emergence of morphogenic blueprint in metazoan animals reflects restricted autonomy of individual cells in adoption of fate outcomes such as differentiation. Fates of individual cells are linked to and influenced by fates of neighboring cells at the population level. Such coupling is a common property of all self-organising systems and propels emergence of order from simple interactions between individual cells without supervision by external directing forces. As a consequence of coupling, expected functional relationship between the constituent cells of an organ system is robustly established concurrent with multiple rounds of cell division during morphogenesis. Notably, the molecular regulation of multicellular coupling during morphogenic self-organisation remains largely unexplored. Here, we review the existing literature on multicellular self-organisation with particular emphasis on recent discovery that β-catenin is the key coupling factor that programs emergence of multi-cellular self-organisation by regulating synchronised cycling of individual cells.

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Section: Molecular Basis Of Coupled Cyclingmentioning

confidence: 99%

Coupled cycling and regulation of metazoan morphogenesis

Rezaei-Lotfi

Farahani

2020

Cell Div

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“…Human brain pericytes, with a demonstrated neural differentiation capacity [15], were purchased from ScienCell (Carlsbad, CA). DMEM/F12 supplemented with 10% fetal calf serum, recombinant human FGF-2 20 ng/mL (R&D Systems, 233-FB) and Antibiotic-Antimycotic (100X, Life Technologies) were used for culturing the neural progenitors for analysis of mitotic cycle.…”

Section: Cell Culturementioning

confidence: 99%

“…Cells were passaged every 24 h at 70-80% confluence stage. Experiments were performed at passages 8-10 [15].…”

Section: Cell Culturementioning

confidence: 99%

“…We used human neural progenitors [15] with endogenous (mi end ct.) miR4673 activity ( Figure 1a) to investigate the dynamics of miRNA signaling. Amplification of the endogenous miRNA was achieved by application of the pre-miRNA cloned into pGeneClip-U1 vector (mi high Δpl, 100 nM/2 × 10 6 cells) or the naked pre-miRNA (mi high Δmi, 200 nM/2 × 10 6 cells).…”

Section: Mirna4673 Instructs Bimodal Reprogramming Of Cell Cyclementioning

confidence: 99%

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Bi-modal reprogramming of cell cycle by MiRNA-4673 amplifies human neurogenic capacity

et al. 2019

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Molecular mechanisms that inform heterochronic adaptations of neurogenesis in Homo sapiens remain largely unknown. Here, we uncover a signature in the cell cycle that amplifies the proliferative capacity of human neural progenitors by input from microRNA4673 encoded in Notch-1. The miRNA instructs bimodal reprogramming of the cell cycle, leading to initial synchronization of neural precursors at the G0 phase of the cell cycle followed by accelerated progression through interphase. The key event in G0 synchronization is transient inhibition by miR4673 of cyclin-dependent kinase-18, a member of an ancient family of cyclins that license M-G1 transition. In parallel, autophagic degradation of p53/p21 and transcriptional silencing of XRCC3/BRCA2 relax G1/S cell cycle checkpoint and accelerate interphase by ≈2.8-fold. The resultant reprogrammed cell cycle amplifies the proliferative capacity and delays the differentiation of human neural progenitors. ARTICLE HISTORY

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“…Human neural progenitors (Farahani et al, 2019b) were purchased from ScienCell ® (Carlsbad, CA). DMEM/F12 supplemented with 10% fetal calf serum (FCS), recombinant human FGF-2 (20 ng/ml, R&D Systems) and Antibiotic-Antimycotic ® (100X, Life Technologies) was used for culturing the cells.…”

Section: Cell Culturementioning

confidence: 99%

Genomic Competition for Noise Reduction Shaped Evolutionary Landscape of Mir-4673

Farahani

Rezaei-Lotfi

Hunter

2019

Preprint

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AbstractThe genomic platform that informs evolution of microRNA cascades remains unknown. Here we capitalized on the recent evolutionary trajectory of hominin-specific miRNA-4673 (Dokumcu et al., 2018) encoded in intron 4 of notch-1 to uncover the identity of one such precursor genomic element and the selective forces acting upon it. The miRNA targets genes that regulate Wnt/β-catenin signalling cascade. Primary sequence of the microRNA and its target region in Wnt modulating genes evolved from homologous signatures mapped to homotypic cis-clusters recognised by TCF3/4 and TFAP2A/B/C families. Integration of homologous TFAP2A/B/C cis-clusters (short range inhibitor of β-catenin (Li and Dashwood, 2004)) into the transcriptional landscape of Wnt cascade genes can reduce noise in gene expression (Blake et al., 2003). Probabilistic adoption of miRNA secondary structure by one such cis-signature in notch-1 reflected selection for superhelical curvature symmetry of precursor DNA to localize a nucleosome that overlapped the latter cis-cluster. By replicating the cis-cluster signature, non-random interactions of the miRNA with key Wnt modulator genes expanded the transcriptional noise buffering capacity via a coherent feed-forward loop mechanism (Hornstein and Shomron, 2006). In consequence, an autonomous transcriptional noise dampener (the cis-cluster/nucleosome) evolved into a post-transcriptional one (the miRNA). The findings suggest a latent potential for remodelling of transcriptional landscape by miRNAs that capitalize on non-random distribution of genomic cis-signatures.

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Neural microvascular pericytes contribute to human adult neurogenesis

Cited by 30 publications

References 67 publications

Coupled cycling and regulation of metazoan morphogenesis

Coupled cycling and regulation of metazoan morphogenesis

Bi-modal reprogramming of cell cycle by MiRNA-4673 amplifies human neurogenic capacity

Genomic Competition for Noise Reduction Shaped Evolutionary Landscape of Mir-4673

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