Neural Stem Cell-Conditioned Medium Protects Neurons and Promotes Propriospinal Neurons Relay Neural Circuit Reconnection after Spinal Cord Injury

Peng, Liang; Liu, Jiaren; Xiong, Jinsheng; Liu, Qing; Zhao, Jiaxin; Liang, Hongsheng; Zhao, Liwei; Tang, Haitao

doi:10.3727/096368914x684989

Cited by 29 publications

(43 citation statements)

References 49 publications

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“…suggesting that CM-hNSC has a beneficial effect on the survival of differentiated SH-SY5Y cells. A previous in vivo study by other investigators showed that CM-hNSCs can reduce the number of caspase 3-positive apoptotic profiles of spinal cord injuries [11]. Consistently, our results further confirmed that the decreased number of differentiated SH-SY5Y cells is due to apoptosis resulting from RA treatment, whereas CM-hNSCs can protect these RA-differentiated SH-SY5Y cells --from apoptosis, as demonstrated by the PI/annexin V staining results ( Fig.…”

Section: Ra-differentiated Sh-sy5y Cellssupporting

confidence: 91%

A new method to effectively and rapidly generate neurons from SH-SY5Y cells

Hong

Wang

Sun

et al. 2016

Neuroscience Letters

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It is well known that neurons differentiated from SH-SY5Y cells can serve as cell models for neuroscience research; i.e., neurotoxicity and tolerance to morphine in vitro. To differentiate SH-SY5Y cells into neurons, RA (retinoic acid) is commonly used to produce the inductive effect. However, the percentage of neuronal cells produced from SH-SY5Y cells is low, either from the use of RA treatment alone or from the combined application of RA and other chemicals. In the current study, we used CM-hNSCs (conditioned medium of human neural stem cells) as the combinational inducer with RA to prompt neuronal differentiation of SH-SY5Y cells. We found that neuronal differentiation was improved and that neurons were greatly increased in the differentiated SH-SY5Y cells using a combined treatment of CM-hNSCs and RA compared to RA treatment alone. The neuronal percentage was higher than 80% (about 88%) on the 3rd day and about 91% on the 7th day examined after a combined treatment with CM-hNSCs and RA. Cell maturation and neurite growth of these neuronal cells were also improved. In addition, the use of CM-hNSCs inhibited the apoptosis of RA-treated SH-SY5Y cells in culture. We are the first to report the use of CM-hNSCs in combination with RA to induce neuronal differentiation of RA-treated SH-SY5Y cells. Our method can rapidly and effectively promote the neuronal production of SH-SY5Y cells in culture conditions.

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Section: Ra-differentiated Sh-sy5y Cellssupporting

confidence: 91%

A new method to effectively and rapidly generate neurons from SH-SY5Y cells

Hong

Wang

Sun

et al. 2016

Neuroscience Letters

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“…Cantinieaux et al reported that BMSC-CM administered after spinal cord contusion improves motor recovery in rats (18). Moreover, Liang et al showed that neural stem cell conditioned medium promoted locomotor recovery in adult rats after spinal cord injury (29). In addition, Torrente et al reported that paracrine factors of human mesenchymal stem cells are a useful neuroprotective approach for brain recovery following injury (30).…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of bone marrow-derived mesenchymal stem cellsand conditioned medium in mechanically injured neuroblastoma cells

et al. 2016

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IntroductionStem cells exhibit self-renewal characteristics that distinguish them from other cell types. Major subtypes include totipotent cells, which can divide to produce all cells in an organism, pluripotent cells, which are able to differentiate into any of the three germ layers (endoderm, ectoderm, or mesoderm), and multipotent cells, which can produce multiple cell types within a given lineage (1). Mesenchymal stem cells (MSCs) constitute a class of multipotent cells capable of differentiating into many diverse cell types, including neural cells, chondroblasts, adipocytes, bone, skeletal muscle, and connective tissue (2-4). These cells can be isolated from almost any tissue, including bone marrow, Wharton jelly, umbilical cord, placenta, dental pulp, and adipose tissue using a simple procedure (5). Identification of MSCs is primarily performed using specific phenotypic and cell surface markers. MSCs should be able to adhere to a plastic surface and express defined surface antigens such as CD29, CD73, CD90, and CD105; however, they should also be negative Background/aim: Bone marrow-derived mesenchymal stem cells (BMSCs) possess self-renewal characteristics that distinguish them from other cell types. Recent studies have focused on the effects of conditioned medium (CM) that includes the extracellular matrix.Here we examined the neuroprotective effects of BMSCs and CM on damaged neuroblastoma cells. Materials and methods:The cells were divided into five groups: 1) healthy controls, 2) damaged cells alone, 3) damaged cells treated with BMSCs, 4) damaged cells treated with CM, and 5) damaged cells treated with both BMSCs and CM. Neuroprotective effects were then evaluated based upon the levels of oxidative stress, antitransforming growth factor β1 (anti-TGFβ1) production, and apoptosis.Results: Significant differences were observed between healthy controls and damaged cells (P < 0.001), as well as between damaged cells and those treated with BMSCs alone (P < 0.05), CM alone (P < 0.05), and both BMSCs and CM in combination (P < 0.01). Among the treated groups, the strongest neuroprotective effects were seen in cells treated with both BMSCs and CM. Conclusion:These results show that both BMSCs and CM exhibit neuroprotective effects in damaged neuroblastoma cells. The strongest benefits were seen following treatment with both BMSCs and CM.

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“…Despite the initial perception that NSC transplantation only served to replace lost cells, recent experimental studies have shown that transplanted NSCs also exert additional bystander effects such as the neuroprotection that promote tissue repair (Aharonowiz et al, 2008). To verify this characteristic of NSCs, Liang et al (2014) removed the neural stem cell from a normal culture medium to acquire neural stem cell-conditioned medium to exclude the effect of cell substitution. Results showed that continuous administration of conditioned medium in rats with spinal cord injury increases bridging between the corticospinal tract and intermediate neurons reduces neuron apoptosis, and promotes the recovery of motor function.…”

Section: Protect Neuronsmentioning

confidence: 99%

Neural stem cell secretome and its role in the treatment of neurodegenerative disorders

Zhang,

Li,

et al. 2020

J. Integr. Neurosci.

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Neurological diseases in the central nervous system are mostly characterized by the failure of endogenous repair to restore tissue damage and salvage lost function. Currently, studies have shown that neural stem cell transplantation provides a good therapeutic effect on neurological diseases. For this reason, neural stem cell transplantation has been explored as a cell replacement therapy. Although transplanted cells can replace cells lost during or post central nervous system injury, many studies have shown that this mechanism is insufficient as most of these newly formed cells fail to integrate and eventually die. Although it was initially thought that neural stem cell could only replace lost cells, recent experiments have shown that transplanted neural stem cell can also play bystander roles such as neuroprotection and immune regulation, promote tissue repair by preventing tissue damage, interfere with pathogenic processes, or by rescuing endogenous nerve cells. However, compelling evidence has raised concerns about this bystander effect, which can be caused by several biologically active molecules (collectively known as the secretome) produced by neural stem cells. These results also raise the possibility of the neural stem cell secretome as a potential candidate for neural stem cell transplantation therapies based on the bystander effect. A better understanding of the molecules and mechanisms of this effect is of critical importance for neural stem cell-based therapies. This review aims to discuss the function and application of neural stem cell secretome in the treatment of neurodegenerative disorders.

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Neural Stem Cell-Conditioned Medium Protects Neurons and Promotes Propriospinal Neurons Relay Neural Circuit Reconnection after Spinal Cord Injury

Cited by 29 publications

References 49 publications

A new method to effectively and rapidly generate neurons from SH-SY5Y cells

A new method to effectively and rapidly generate neurons from SH-SY5Y cells

Neuroprotective effects of bone marrow-derived mesenchymal stem cellsand conditioned medium in mechanically injured neuroblastoma cells

Neural stem cell secretome and its role in the treatment of neurodegenerative disorders

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