Neuraminidase-Activatable NIR Fluorescent Probe for Influenza Virus Ratiometric Imaging in Living Cells and Colorimetric Detection on Cotton Swabs

Abstract

Influenza, as one of the most serious communicable respiratory infectious diseases, is caused by influenza viruses. Neuraminidase (NA), also referred to as sialidase, is an important protein on the influenza virus surface. Because of its enzymatic function, NA has been selected as a promising target to develop influenza virus diagnosis reagents or drugs. Herein, a neuraminidase-responsive, light-up, near-infrared (NIR) fluorescence probe (SA-DCM) was reported that possesses both ratiometric and colormetric properties for different applications. Benefitting from the ratiometric properties, SA-DCM showed an increased anti-interference capacity for environmental fluctuation and enhanced detection accuracy with a large Stokes shift (>150 nm) and outstanding sensitivity and selectivity. Due to its colorimetric property, a color shift of the product HO-DCM from yellow to purple occurs when the pH value is adjusted. Therefore, a colorimetric approach through direct color conversion on a cotton swab to detect NA was established, and the result could be directly read by the naked eye with simplicity and practicality.

Discovering Neuraminidase Inhibitors via Computational and Experimental Studies

“…New strains of the viruses continue to emerge and the risk of drug resistance have sparked great interest into finding potential anti-viral compounds. [9][10][11][12][13][14][15] Neuraminidase is a key surface glycoprotein which plays an important role in viral replication and infection. It is a proven target for developing drugs against influenza A viruses [16][17] .…”

MedChemExpress compounds prevent neuraminidase N1 via physics- and knowledge-based methods

“…Numerous studies were carried out to nd a potential inhibitor for treating the disease. [9][10][11][12][13][14][15] In particular, neuraminidase is the most popular drug target since it is an important element in the delivery of viral progeny to human cells. 16 Oseltamivir, zanamivir, and peramivir are among the drugs approved for inhibiting the target.…”

The first specific probe for pyrrolidine with multifunction by the interaction mechanism of atomic economic reaction

“… 11 The optical properties of the probe are changed by the chemical reaction between the analyte and the probe, 12 so it has great advantages in specificity. 13 Among the reactive fluorescent probes, usually, the probes with the interaction mechanism of the decomposition-type reaction are common, 14 and have been used to specifically detect H 2 O 2 , 15 H 2 S, 16 and enzymes 17 in living cells. In contrast, the reactive probes that are directly combined with the analyte to produce a substance are relatively rarely reported, 18 and most of them are used to detect inorganic small molecule species, such as SO 2 , 19 NO, 20 H + , 21 Hg 2+ , 22 etc.…”