NEUREC — a program package for 3D-reconstruction from serial sections using a microcomputer

Gras, Heribert; Killmann, F.

doi:10.1016/0010-468x(83)90035-1

Cited by 19 publications

(3 citation statements)

References 8 publications

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“…Synapse counting and three-dimensional computer-aided reconstructions (Gras 1984) of the DCMD and thick profiles of the LGMD were performed by help of a graphics tablet and a PC (Gras and Killmann 1983;Gras 1984), with custom programs (Gras unpublished; POLYRAY shareware by Alexander Enzmann). For this purpose, we took thin sections of every third of the re-embedded semi-thin sections, inspected and photographed by the electron microscope.…”

Section: Figmentioning

confidence: 99%

Types, numbers and distribution of synapses on the dendritic tree of an identified visual interneuron in the brain of the locust

Killmann

Gras

Schürmann

1999

Cell and Tissue Research

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The descending contralateral movement detector (DCMD), an identified descending interneuron in the brain of the locust Schistocerca gregaria has been investigated by using light and electron microscopy. We describe the fine structure, distribution and numbers of synapses that it receives from another identified brain neuron, the lobular giant movement detector (LGMD), and from unidentified neurons. The DCMD dendrites emerging from the integrative segment vary in form and number between individuals and sexes but always form a flattened dendritic domain. The arborizations and the integrative segment appear to be exclusively postsynaptic. Two types of synaptic contacts (Type 1 and 2) onto the DCMD can be discerned as having either round (Type 1) or pleiomorphic synaptic vesicles (Type 2) and by large (Type 1) or small (Type 2) subsynaptic appositions. Contact zones of Type 1 synapses are smaller than those of Type 2. LGMD-synapses are of Type 1 and occur intermingled with presynaptic sites of unidentified units. Some branches of the DCMD receiving input from unidentified units are devoid of contacting LGMD processes. Synapses of both types are randomly distributed over the DCMD integrative segment and at fibres with similar sizes. Type 1 synapses are much more frequent than Type 2 synapses and their number is negatively correlated with fibre diameter. For a whole DCMD dendritic arborization, a total of 8500 active zones of chemical synapses has been calculated, including a minimum of 2250 LGMD-synapses and about 1000 Type 2 synapses. The DCMD may thus receive a considerable amount of input from as yet unidentified neurons.

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Section: Figmentioning

confidence: 99%

Types, numbers and distribution of synapses on the dendritic tree of an identified visual interneuron in the brain of the locust

Killmann

Gras

Schürmann

1999

Cell and Tissue Research

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“…The profiles of individual Po on acetate sheets were traced by hand on an Apple graphic tablet and the coordinates were entered into the files of an Apple IIe microcomputer (Apple Computer, Inc., Cupertino, CA). The details of the computerbased 3-D reconstruction program NEUREC have been reported elsewhere (16,17). The reconstructed 3-D images were viewed from different angles on the monitor of the microcomputer and were printed out using a printer (FX-80; Epson, Tokyo, Japan).…”

Section: Serial Section Studies and 3-d Reconstruction Of Pomentioning

confidence: 99%

Three-dimensional reconstruction of a peroxisomal reticulum in regenerating rat liver: evidence of interconnections between heterogeneous segments.

Yamamoto¹,

Fahimi²

1987

The Journal of Cell Biology

119

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Abstract. The three-dimensional (3-D) form and the interrelationship of peroxisomes (Po) in the model of regenerating rat liver after partial hepatectomy were studied by computer-assisted 3-D reconstruction of serial ultrathin sections. Po were labeled cytochemically for either catalase, which stains them all uniformly, or for D-amino acid oxidase (DAA-OX), which gives a heterogeneous reaction with lightly and darkly stained PO. In regenerating rat liver, Po exhibit marked pleomorphism with some budding forms and dumbbellshaped ones. The 3-D reconstruction revealed many single spherical Po measuring 0.15-0.8 l~m in diameter. In addition, two to five Po were found interconnected with each other via narrow 30-50-nm hourglass-shaped bridges forming a reticulum. Such aggregates of Po measured 1.5-2.5 Ixm across. Whereas all segments of this reticulum stained homogeneously for catalase, they exhibited a marked difference in the intensity of the DAA-OX reaction. These observations are consistent with the view of peroxisomal proliferation by budding or fragmentation from preexisting ones. Under such conditions of rapid growth as in regenerating liver, Po may be interconnected forming a reticulum. The interconnections between Po with differing DAA-OX activities suggest that they originate from the same parent organelle.

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“…The purpose of this report is to describe the computer From University Hospitals of Cleveland, Case Western Reserve University. Presented at the Fifteenth Annual Meeting of the Midwestern Vascular Surgical Society, Chicago, Ill., Sept. [20][21]1991. Reprint requests: Jeffrey R. Rubin, MD, 2074 Abington Rd., Cleveland, OH 44106.…”

mentioning

confidence: 99%

A new approach for three-dimensional reconstruction of arterial ultrasonography

Franceschi

Bondi

Rubin

1992

Journal of Vascular Surgery

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This report describes a computerized approach that 'allows the creation of realistic three-dimensional arterial images from two-dlmensional contiguous slices derived from a conventional ultrasotmd scan. Furthermore, the study assesses the method's feasibility and accuracy by performing in vitro cadaver artery three-dimensional reconstructions. Images are digitized into a computer, with a resolution of 512 x 480 pixels, and a dynamic range of 8 bits/pixel (256 gray scale). After edge enhancement with convolution filters through the original binary data, the intraluminal and outer edges are traced and converted to a polygon vector within a defined three-dimensional space. Serial cuts, 2 mm apart, are then "stacked" into a three-dimensional model, with interpolation of polyhedra between slices. Sixteen normal and arteriosclerotic distal aortic and common iliac arteries were obtained from fresh cadavers. Three-dimensional reconstruction models were compared with gross examination of the original artery. Anatomically accurate reconstructions were obtained, all with detailed surface information. Data analyzed included diameter, area, residual lumen, and percent stenosis. Pearson's coefficients determined for the pairs of data indicated excellent correlation ( ___ 0.90) between separate measurements. With use of the described technology it is feasible to perform arterial three-dimensional reconstructions on a personal computer, with detailed and accurate surface information. The threedimensional reconstruction method used can reliably and consistently reproduce the anatomic specimens. (

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NEUREC — a program package for 3D-reconstruction from serial sections using a microcomputer

Cited by 19 publications

References 8 publications

Types, numbers and distribution of synapses on the dendritic tree of an identified visual interneuron in the brain of the locust

Types, numbers and distribution of synapses on the dendritic tree of an identified visual interneuron in the brain of the locust

Three-dimensional reconstruction of a peroxisomal reticulum in regenerating rat liver: evidence of interconnections between heterogeneous segments.

A new approach for three-dimensional reconstruction of arterial ultrasonography

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