Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

Bueno, Ednéia Casagranda; Vaz, Adelaide José; Machado, Luı́s dos Ramos; Livramento, José Antônio

doi:10.1590/s0004-282x2000000100003

Cited by 41 publications

(33 citation statements)

References 25 publications

(33 reference statements)

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“…In our material we detected the presence of high levels of specific immunoglobulins in the group with an active form of NCC, with higher IgG positivity, followed, in decreasing order, by positivity to IgM, IgE and IgA, in agreement with data reported by others about the higher participation of IgG in the immune response to NCC 16,19 .…”

Section: Elisa Igesupporting

confidence: 92%

Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

Odashima

Takayanagui

Figueiredo

2002

Arq. Neuro-Psiquiatr.

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-The objective of this study was to analyze different immunoglobulins classes (IgG, IgM, IgE and IgA) against Cysticercus cellulosae in the cerebrospinal fluid (CSF), through enzyme linked immunosorbent assay (ELISA), correlating them to clinical and tomographic profiles in patients with neurocysticercosis (NCC). Eighty-five specimens of CSF were obtained from 43 cases with NCC (26 with the active form and 17 with the inactive form) and from 42 patients with other neurological diseases. The inactive form of NCC presented a profile in CSF similar to the group without NCC. The active form of NCC presented elevation of specific immunoglobulins (IgG, IgM, IgE, and IgA) in decreasing order, with the highest values being detected among the cases with intraventricular cysts, or with inflammation signs in CSF or in those with multiple clinical manifestations. The highest sensitivity and specificity were obtained with ELISA-IgG (88.5% and 93.2%, respectively). This study confirmed the importance of ELISA in the immunologic diagnosis of NCC.

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Section: Elisa Igesupporting

confidence: 92%

Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

Odashima

Takayanagui

Figueiredo

2002

Arq. Neuro-Psiquiatr.

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“…NC does not always present inflammatory changes in the CSF 26,27 , as can be seen in Table 1: (1) intracranial hypertension is observed with similar frequency in all three groups, suggesting the occurrence of the mass effect even in patients with the form that is non-reactive to the CSF exam; (2) an increase in proteins and a decrease in the glucose levels are observed, albeit less markedly, in significant percentages in the non-reactive group.…”

Section: Discussionmentioning

confidence: 73%

“…To characterize local immunoproduction we used Link & Tibbling IgG indices 21 , Tourtellote's daily IgG synthesis 22 , Thompson's nomogram (IgG quotient versus albumin quocient) 23,24 and protein isoelectrofocusing 26 . Anti-Taenia specific antibody indices were calculated by the method established by Reiber and Felgenhauer 7 , using an ELISA assay.…”

Section: Methodsmentioning

confidence: 99%

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

Machado

Livramento

Vaz

et al. 2002

Arq. Neuro-Psiquiatr.

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-We analyzed cerebrospinal fluid (CSF) and blood serum from 55 patients with neurocysticercosis (NC) at different clinical stages. According to inflammatory activity in the CSF, three stages were identified: (1) reactive, when there was at least an increase in the number of cells; (2) weakly reactive, when significant alterations were found in the CSF, including an increase in gamma globulins, albeit without hypercytosis; (3) non-reactive, when there was neither hypercytosis nor increase in gamma globulins. Nineteen patients had the reactive form; 18 had the weakly reactive form; 18 displayed the non-reactive form.

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“…Furthermore, cysticercosis is endemic in many regions of the world and more common in pig eating countries with low socioeconomic status and poor sanitation 3 . HIV infection is a serious problem in the present day world wide.…”

Section: Introductionmentioning

confidence: 99%

A serological study of cysticercosis in patients with HIV

Parija

Gireesh

2009

Rev. Inst. Med. trop. S. Paulo

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Neurocysticercosis (NCC) has attained the importance of one of the most common cause of focal brain lesions in patients infected with HIV (human immunodeficiency virus). Adequate data regarding the rate of this co-infection is lacking. Therefore, the present study was carried out to determine the prevalence of cysticercosis among HIV patients residing in Puducherry or its neighboring districts of Tamil Nadu State, India. A total of one hundred blood samples were collected from HIV seropositive cases visiting JIPMER hospital, Puducherry, between June 2007 and May 2008. Enzyme immunotransfer blot (EITB) and enzyme linked immunosorbent assay (ELISA) were used to demonstrate anti- T. solium larval stage antibodies and Co-agglutination (Co-A) test was used to detect T. solium larval stage antigens in sera. Two HIV seropositive cases were found positive for anti-T. solium larval stage antibody by EITB and four were positive by ELISA. Only one sample was positive by both EITB and ELISA. No serum sample was found positive for T. solium larval stage antigen by Co-A test. The overall seropositivity detected by all the methods was 5% in this study group. The accurate clinical diagnosis of NCC in HIV is difficult due to deranged immunological parameters in the HIV infected patients. The results of this study provides important data on the prevalence of cysticercosis in HIV positive patients in Puducherry and neighboring areas which was previously unknown. This study will also increase awareness among physicians and public health agencies about T. solium cysticercosis in the selected group.

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Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens

Cited by 41 publications

References 25 publications

Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

Enzyme linked immunosorbent assay (ELISA) for the detection of IgG, IgM, IgE and IgA against Cysticercus cellulosae in cerebrospinal fluid of patients with neurocysticercosis

IgG intrathecal synthesis and specific antibody index in patients with neurocysticercosis

A serological study of cysticercosis in patients with HIV

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