Neuroepithelial bodies in the lung of the tree frog, Hyla arborea L.

Goniakowska-Witalińska, Lucyna

doi:10.1007/bf00233593

Cited by 44 publications

(27 citation statements)

References 16 publications

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“…Neuroendocrine cells have been identified in the lungs of birds (Cook & King 1969;Walsh & McLelland 1974;Wasano & Yamamoto 1979) and reptiles (Scheuermann et al 1983;Pastor et al 1987Pastor et al , 1988Pastor et al , 1989. In amphibian lungs they occur both individually or as neuroepithelial bodies (Goniakowska-Witalinska 1980, 1981Cutz et al 1986;Goniakowska-Witalinska & Cutz 1990;Goniakowska-Witalinska et al 1991).…”

Section: Introductionmentioning

confidence: 99%

Immunocytochemical Localization of Serotonin and Neuropeptides in the Neuroendocrine Paraneurons of Teleost and Lungfish Gills*

et al. 1992

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Zaccone, G., Lauweryns, J . M., Fasulo, S., Tagliafierro, G., Ainis. L. & Licala, A . 1992. Immunocytochemical localization of serotonin and neuropeptides in the neuroendocrine paraneurons of teleost and lungfish gills.-Acta Zoologica (Stockholm) 73: 177-183.Neuroendocrine (NE) cells in the gills of six fish species were identified by serotonin and peptide immunohistochemistry. They occur either as single cells or as cell clusters within the filamental epithelium. Two populations of NE cells were identified that exhibit an immunoreactivity either for serotonin alone or for met-enkephalin and leu-enkephalin co-distributed in the same cells. We demonstrate for the first time the occurrence of open-type endocrine cells in the fish gill epithelium and confirm the presence of only serotonin-immunopositive cells of the closed type. The NE cells of the species studied do not react positively to other neuroendocrine markers occurring in the mammalian lung. Great differences probably exist in the expression of these epitopes on antigenic structures among various animal species.Although the physiological role of neuropeptides and serotonin in the fish gill NE cells is unknown, the presence of these molecular markers is of interest not only from an evolutionary aspect, but also in terms of a potential animal model to study the supposed functions of the airway neuroendocrine system in the vertebrates.

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Section: Introductionmentioning

confidence: 99%

Immunocytochemical Localization of Serotonin and Neuropeptides in the Neuroendocrine Paraneurons of Teleost and Lungfish Gills*

et al. 1992

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“…The investigations carried out so far point out that in amphibians the nerve endings are located in the basal part of NEB Haller, 1978, 1980;Wasano and Yamamoto, 1978;Goniakowska-Witalinska, 1981;Matsumura, 1985;Scheuermann et al, 1989;Goniakowska-Witalinska and Cutz, 1990;GoniakowskaWitalinska et al, 1990). The present study revealed a morphologically atypical innervation of NEB in A .…”

mentioning

confidence: 95%

Ultrastructure and immunocytochemistry of the neuroepithelial bodies in the lung of the tiger salamander, Ambystoma tigrinum (Urodela, amphibia)

Goniakowska-Witalińska

Lauweryns

Zaccone

et al. 1992

Anat. Rec.

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Light and electron microscopy of the lungs of Ambystoma tigrinum (Urodela) revealed a relatively complex pattern of the neuroendocrine (NE) cells. In the apical parts of smaller septa single NE cells not associated with nerve fibres were covered and surrounded by pneumocytes. The larger septa possessed small areas of ciliated epithelium, in which the NE cells were grouped in a form of neuroepithelial bodies (NEB) consisting of 3-5 cells and covered by goblet cells. NE cells possessed a large nucleus with patches of condensed chromatin, clear cytoplasm, and membrane-bound vesicles of variable morphology and size, containing an electron dense interior surrounded by a lucent space. The size of these dense core vesicles (DCV) ranged from 70-140 nm, while rarely the larger ones exhibited a diameter of 300-600 nm. In some NEB a second type of NE cells was observed for the first time in an amphibian species: these cells communicated with the air space and exhibited on their surface microvilli and a single modified cilium with a 8 + 1 microtubule arrangement. Their cytoplasm contained two types of DCV: dense core granules with a diameter of 140-260 nm and vesicles 320-700 nm in diameter with a moderately electron dense interior. The NEB were associated with intracorpuscular, sensory nerve terminals morphologically afferent and efferent. By immunocytochemistry, the NE cells revealed the presence of serotonin, met-enkephalin, and leu-enkephalin. A paracrine and chemoreceptor role is proposed for NEB of Ambystoma tigrinum.

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“…However, both the size and electron-density of the DCVs vary greatly, not only from cell to cell, but even within the same cell (e.g. Goniakowska-Witalinska, 1981;Lauweryns et al, 1974). Based on the size and electron density of the DCVs some authors have described at least three, apparently different types of NEE cells in the fetus and the newborn infant (Hage, 1974), whereas only one type was identified in adult human lungs (Capella et al, 1978;Cutz and Orange, 1977;Hage, 1984).…”

Section: Ultrastructure Of Solitary Neuroepithelial Endocrine Cells Amentioning

confidence: 96%

Comparative histology of pulmonary neuroendocrine cell system in mammalian lungs

Scheuermann

1997

Microsc. Res. Tech.

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It is increasingly recognized that neuroepithelial endocrine cells and organoid clusters of these cells termed neuroepithelial bodies are widely distributed in the respiratory tract of vertebrates. This review focuses on the neuroepithelial endocrine system in the airway epithelium of mammals according to observations made in a wide range of species, as it appears in light and electron microscopy by means of various visualization techniques under normal and experimental conditions. Because there are similarities but also marked differences between species, wherever possible studies in human airways are emphasized. Conventional histochemical and ultrastructural studies including microspectrofluorimetry have demonstrated the amine‐handling properties and the presence of neurosecretory granules in neuroendocrine cells of the epithelium of all mammals studied so far. The neuroepithelial bodies are innervated by morphological afferent‐ and efferentlike synaptic specializations. These, together with the presence of reciprocal synapses, emphasize that these cells may be involved in dual functions of chemoreception and secretion. Light and electron microscopic immunohistochemistry has demonstrated that neuroepithelial endocrine cells may contain various biogenic agents, suggesting that the neuroendocrine system is highly heterogeneous. Neural elements may interact in a complex manner, and the activation of certain neural pathways may control the release of biogenic substances to influence physiological airway functions. They may be particularly relevant in pulmonary diseases. Consequently, the function of this system is complex and it is highly probable that many of its aspects are still not elucidated. Microsc. Res. Tech. 37:31–42, 1997. © 1997 Wiley‐Liss, Inc.

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Neuroepithelial bodies in the lung of the tree frog, Hyla arborea L.

Cited by 44 publications

References 16 publications

Immunocytochemical Localization of Serotonin and Neuropeptides in the Neuroendocrine Paraneurons of Teleost and Lungfish Gills*

Immunocytochemical Localization of Serotonin and Neuropeptides in the Neuroendocrine Paraneurons of Teleost and Lungfish Gills*

Ultrastructure and immunocytochemistry of the neuroepithelial bodies in the lung of the tiger salamander, Ambystoma tigrinum (Urodela, amphibia)

Comparative histology of pulmonary neuroendocrine cell system in mammalian lungs

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