Neuroepithelial Stem Cell Proliferation Requires LIS1 for Precise Spindle Orientation and Symmetric Division

Yingling, Jessica; Youn, Yong Ha; Darling, Dawn L.; Toyo-oka, Kazuhito; Pramparo, Tiziano; Hirotsune, Shinji; Wynshaw‐Boris, Anthony

doi:10.1016/j.cell.2008.01.026

Cited by 262 publications

(334 citation statements)

References 43 publications

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“…However, in contrast with these results, a hypomorphic allele of Lis1 did not lead to abnormalities in interkinetic nuclear movements, although the morphology of the radial glia was aberrant (Cahana et al 2001). Further reduction of LIS1 resulted in abolishment of interkinetic nuclear oscillations in the radial glial progenitors (Tsai et al 2005;Yingling et al 2008). Conditional knockout of Lis1 in neural stem cells revealed a difference in the sensitivity to LIS1 function in neuroepithelial stem cells versus radial glia (Yingling et al 2008).…”

Section: Molecular Motors and The Cytoskeletoncontrasting

confidence: 40%

“…Further reduction of LIS1 resulted in abolishment of interkinetic nuclear oscillations in the radial glial progenitors (Tsai et al 2005;Yingling et al 2008). Conditional knockout of Lis1 in neural stem cells revealed a difference in the sensitivity to LIS1 function in neuroepithelial stem cells versus radial glia (Yingling et al 2008). In the neuroepithelial stem cells, where cells divide in a symmetrical fashion, LIS1 abolishment resulted in a rapid response, where spindle orientation was disturbed and was followed by apoptotic cell death.…”

Section: Molecular Motors and The Cytoskeletonmentioning

confidence: 99%

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Interkinetic Nuclear Movement in the Ventricular Zone of the Cortex

2011

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The nuclei of neuroepithelial cells move along the apicobasal axis in synchronization with their cell cycle status. This motility is known as interkinetic nuclear movement. We discuss here the importance of cytoskeleton organization, the centrosome, molecular motors, cell polarity proteins, and their regulators in controlling and maintaining this typical behavior. Furthermore, due to the tight linkage between cell proliferation, cell cycle, and nuclear motility, we speculate that interkinetic nuclear movement is likely to be affected in the pathophysiology of microcephaly, where the brain size is markedly reduced.

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Section: Molecular Motors and The Cytoskeletoncontrasting

confidence: 40%

Section: Molecular Motors and The Cytoskeletonmentioning

confidence: 99%

Interkinetic Nuclear Movement in the Ventricular Zone of the Cortex

2011

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“…It is known that the horizontal orientation of the cleavage plane of mitotic apical progenitors is essential for early symmetric expansion of NPCs (Yingling et al , 2008; Li et al , 2011). After sufficient NPCs expansion, neurogenesis then begins, which requires switching of the cleavage plane from horizontal to vertical (Rakic, 1995; Lancaster et al , 2013).…”

Section: Resultsmentioning

confidence: 99%

CPAP promotes timely cilium disassembly to maintain neural progenitor pool

et al. 2016

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A mutation in the centrosomal‐P4.1‐associated protein (CPAP) causes Seckel syndrome with microcephaly, which is suggested to arise from a decline in neural progenitor cells (NPCs) during development. However, mechanisms of NPCs maintenance remain unclear. Here, we report an unexpected role for the cilium in NPCs maintenance and identify CPAP as a negative regulator of ciliary length independent of its role in centrosome biogenesis. At the onset of cilium disassembly, CPAP provides a scaffold for the cilium disassembly complex (CDC), which includes Nde1, Aurora A, and OFD1, recruited to the ciliary base for timely cilium disassembly. In contrast, mutated CPAP fails to localize at the ciliary base associated with inefficient CDC recruitment, long cilia, retarded cilium disassembly, and delayed cell cycle re‐entry leading to premature differentiation of patient iPS‐derived NPCs. Aberrant CDC function also promotes premature differentiation of NPCs in Seckel iPS‐derived organoids. Thus, our results suggest a role for cilia in microcephaly and its involvement during neurogenesis and brain size control.

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“…The Rac activator Tiam1, a T-lymphoma invasion and metastasis-inducing protein, acts as a crucial component of the Par complex in regulating neuronal and epithelial apical-basal polarity (Chen and Macara, 2005;Mertens et al, 2005;Nishimura et al, 2005). Lis1 (-subunit of plateletactivating factor acetylhydrolase) is essential for proliferation and the precise control of mitotic spindle orientation in both neuroepithelial stem cells and radial glial progenitor cells (Yingling et al, 2008;Yamada et al, 2010). Expression of both Tiam1 and Lis1 mRNA is decreased in Sftpc:miR302/367 transgenic lungs (Fig.…”

Section: Regulation Of Endoderm Progenitor Apical-basal Polarity By Mmentioning

confidence: 99%

Regulation of lung endoderm progenitor cell behavior by miR302/367

et al. 2011

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SUMMARYThe temporal and spatial control of organ-specific endoderm progenitor development is poorly understood. miRNAs affect cell function by regulating programmatic changes in protein expression levels. We show that the miR302/367 cluster is a target of the transcription factor Gata6 in mouse lung endoderm and regulates multiple aspects of early lung endoderm progenitor development. miR302/367 is expressed at early stages of lung development, but its levels decline rapidly as development proceeds. Gain-and loss-of-function studies show that altering miR302/367 expression disrupts the balance of lung endoderm progenitor proliferation and differentiation, as well as apical-basal polarity. Increased miR302/367 expression results in the formation of an undifferentiated multi-layered lung endoderm, whereas loss of miR302/367 activity results in decreased proliferation and enhanced lung endoderm differentiation. miR302/367 coordinates the balance between proliferation and differentiation, in part, through direct regulation of Rbl2 and Cdkn1a, whereas apical-basal polarity is controlled by regulation of Tiam1 and Lis1. Thus, miR302/367 directs lung endoderm development by coordinating multiple aspects of progenitor cell behavior, including proliferation, differentiation and apical-basal polarity.

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Neuroepithelial Stem Cell Proliferation Requires LIS1 for Precise Spindle Orientation and Symmetric Division

Cited by 262 publications

References 43 publications

Interkinetic Nuclear Movement in the Ventricular Zone of the Cortex

Interkinetic Nuclear Movement in the Ventricular Zone of the Cortex

CPAP promotes timely cilium disassembly to maintain neural progenitor pool

Regulation of lung endoderm progenitor cell behavior by miR302/367

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