Neurohistologic and Ultrastructural Lesions in Cattle Experimentally Intoxicated with the Plant <i>Prosopis juliflora</i>

Tabosa, Ivon M.; Riet-Correa, F.; Barros, Severo Sales de; Summers, B. A.; Simões, Sara Vilar Dantas; Medeiros, Rosane M.T.; Nobre, V. M. T.

doi:10.1354/vp.43-5-695

Cited by 38 publications

(60 citation statements)

References 4 publications

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“…Neurohistologic and ultrastructural lesions in cattle experimentally intoxicated with Prosopis juliflora were characterized by vacuolation and swollen mitochondria, with the mitochondrial cristae displaced peripherally, disoriented and disintegrating in neurons of the motor cranial nerve nucleus (Tabosa et al 2006). Studies in isolated mitochondria of rat brains showed that juliprosopine stimulates stage-4 cellular respiration and alters the mitochondrial membrane potential and ATP production by inhibiting complex I of the respiratory chain (Maioli et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)

Silva

Cuevas

Muñoz

et al. 2017

An. Acad. Bras. Ciênc.

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Prosopis juliflora is a shrub that has been used to feed animals and humans. However, a synergistic action of piperidine alkaloids has been suggested to be responsible for neurotoxic damage observed in animals. We investigated the involvement of programmed cell death (PCD) and autophagy on the mechanism of cell death induced by a total extract (TAE) of alkaloids and fraction (F32) from P. juliflora leaves composed majoritary of juliprosopine in a model of neuron/glial cell co-culture. We saw that TAE (30 µg/mL) and F32 (7.5 µg/mL) induced reduction in ATP levels and changes in mitochondrial membrane potential at 12 h exposure. Moreover, TAE and F32 induced caspase-9 activation, nuclear condensation and neuronal death at 16 h exposure. After 4 h, they induced autophagy characterized by decreases of P62 protein level, increase of LC3II expression and increase in number of GFP-LC3 cells. Interestingly, we demonstrated that inhibition of autophagy by bafilomycin and vinblastine increased the cell death induced by TAE and autophagy induced by serum deprivation and rapamycin reduced cell death induced by F32 at 24 h. These results indicate that the mechanism neural cell death induced by these alkaloids involves PCD via caspase-9 activation and autophagy, which seems to be an important protective mechanism.

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Section: Discussionmentioning

confidence: 99%

Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)

Silva

Cuevas

Muñoz

et al. 2017

An. Acad. Bras. Ciênc.

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“…Anteriormente, porém, já se havia comprovado que a ingestão das vagens dessa planta era responsável pela ocorrência de uma enfermidade denominada "jaw and tongue trouble" em bovinos nos Estados Unidos (Dollahite 1964, Kingsbury 1964) e "coquera" em caprinos no Peru (Bacca et al 1963/66). A intoxicação ocorre, também, com o denominado farelo de algaroba, que se obtém por moagem das vagens após secagem adequada (Tabosa et al 2006). No Brasil, a doença foi descrita em bovinos no Rio Grande do Norte (Silva et al 2006), Paraíba e Pernambuco (Dantas & Menezes 1994), e em caprinos na Paraíba (Lima et al 2004).…”

Section: Introductionunclassified

“…Os sinais clínicos, mais evidentes durante a ruminação, são característicos de disfunção de nervos cranianos, principalmente devido à degeneração e desaparecimento dos neurônios do núcleo motor do trigêmeo (Tabosa et al 2000a(Tabosa et al , 2006. Observa-se relaxamento da mandíbula, desvio lateral da cabeça durante a mastigação e ruminação, incoordenação dos movimentos mastigatórios, dificuldade de apreensão dos alimentos, salivação profusa, disfagia e atrofia dos masseteres.…”

Section: Introductionunclassified

Intoxicação espontânea por vagens de Prosopis juliflora (Leg. Mimosoideae) em bovinos no Estado de Pernambuco

et al. 2009

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233Pesq. Vet. Bras. 29(3):233-240, março 2009 RESUMO.-Descrevem-se três surtos de intoxicação por vagens de Prosopis juliflora no Sertão e Agreste de Pernambuco, na região semi-árida, em animais pastejando áreas invadidas pela planta ou que ingeriram as vagens como alimento concentrado. Em duas fazendas nas que a doença ocorria esporadicamente foram observados casos individuais. Em outra, o surto afetou um rebanho de 1206 bovinos, dos quais adoeceram 112 (9,28%) e morreram 84 (6,96%), enquanto os demais 28 (2,32%) recuperaram-se e ganharam peso após a retirada das vagens da alimentação. Clinicamente observou-se, principalmente, perda de peso progressiva, atrofia da musculatura da face e masseter, mandíbula pendulosa, protrusão de língua, dificuldade de apreensão e mastigação dos alimentos, torção da cabeça para mastigar ou ruminar, salivação excessiva, disfagia e hipotonia lingual. Nos exames laboratoriais constatou-se anemia e hipoproteinemia. Three outbreaks of poisoning by Prosopis juliflora pods are reported in the semiarid region of the state of Pernambuco, Northeastern Brazil, in cattle grazing in fields invaded by the plant or ingesting mesquite beans as a concentrate food. In two farms the disease occurred sporadically. In another, 112 (9.28%) cattle out of 1206 were affected, 84 (6.96%) died due to emaciation, and 28 (2.32%) gained weight after the pods had been withdrawn from the feed. Main clinical signs were progressive weight loss, atrophy of the masseter muscles, dropped jaw, tongue protrusion, difficulties in prehending food, tilting the head during mastigation or rumination, salivation, impaired swallowing, and decreased tone of the tongue. The hematology reveals hypoproteinemia and anemia. Gross lesions were emaciation and reduction in size of the masseter muscles, which appear thinner than normal and grayish due muscular atrophy. Degeneration of neurons of the trigeminal motor nuclei, Wallerian degeneration of the trigeminal nerve roots, and muscular atrophy of the masseter muscles with substitution by fibrous tissue were observed on histologic examination. For the prevention of the poisoning is necessary to limit the amount of mesquite beans in animal nutrition. It is also necessary to develop research to determine the economic and sustainability of the use of Prosopis juliflora for animal food, human food or other uses such as charcoal, wood and fuel wood. Intoxicação espontânea por vagens de

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“…A doença foi reproduzida experimentalmente em bovinos que ingeriram rações que continham 50% e 100% da alimentação após um período de seis meses (Figueiredo et al 1996), enquanto Tabosa et al (2006) obtiveram a intoxicação após ingestão de dietas com 50% e 75% das vagens durante 45-75 dias e em caprinos com 60-90% de vagens na alimentação por um período de aproximadamente 210 dias (Tabosa et al 2000a).…”

Section: Introductionunclassified

Efeitos teratogênicos de Prosopis juliflora em ratos e análise da toxicidade das vagens

et al. 2014

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RESUMO.-Objetivou-se com este estudo determinar os possíveis efeitos teratogênicos de Prosopis juliflora, verificar se existe perda da toxicidade entre vagens armazenadas e recém-coletadas e determinar se existe diferença de toxicidade entre as vagens coletadas em diferentes localidades. Trinta ratas prenhes da linhagem Wistar foram separadas, aleatoriamente, em cinco grupos: um controle (G1) e quatro experimentais (G2, G3, G4 e G5). Os animais dos grupos G2 e G3 foram alimentados com ração contendo 70% de vagens de P. juliflora recém-coletadas em dois municípios diferentes. Os grupos G4 e G5 foram alimentados com ração preparada com vagens das mesmas procedências, porém armazenadas por um período de 6 meses. O grupo controle recebeu ração sem vagens de P. juliflora. No grupo controle o número de malformações por ninhada (1,16 ± 0,98) foi significativamente menor do que os dos grupos experimentais (14,00 ± 2,96, 6,16 ± 2,22, 7,66 ± 2,94 e 4,66 ± 1,63 para os grupos G2, G3, G4 e G5, respectivamente) indicando que a planta é teratogênica. Não foram observadas diferenças significativas na frequência de malformações e no número de fetos nascidos entre os grupos que receberam vagens de diferentes localidades. No entanto, o número de malformações nos grupos que receberam as vagens recém-colhidas (65,80 ± 21,20 a, 67,59 ± 18,10 a), foi significativamente maior que o observado nas ratas que receberam as vagens após o armazenamento (35,74±10,10b, 21,85±5,13c) The objective of this study was to determine the possible teratogenic effects of Prosopis juliflora, check if there is a loss in toxicity between pods stored and newly collected and determine whether there are differences in toxicity between the pods collected in different localities. Thirty pregnant female Wistar rats were randomly separated into five groups: a control (G1) and four experimental (G2, G3, G4 and G5), each with six animals. The animals in groups G2 and G3 were fed diets containing 70% of pods of P. juliflora newly collected in two different municipalities. The groups G4 and G5 were fed beans prepared with the same origins, but stored for a period of 6 months. The control group received the same diet without pods of P. juliflora. In the control group the number of defects per litter (1.16 ± 0.98) was significantly lower than the experimental groups (14.00 ± 2.96, 6.16 ± 2.22, 7.66 ± 2.94 and 4.66 ± 1.63 for G2, G3, G4 and G5, respectively) indicating that the plant is teratogenic. No significant differences were observed in the frequency of malformations and number of fetuses born between groups receiving pods from different locations. However, the number of defects in the groups who received the freshly harvested pods was significantly different from the number observed in rats that received the beans after storage, suggesting that the teratogenic effect of the plant decreases during storage. We conclude that the pods of P. juliflora are teratogenic for Wistar rats and that the teratogenicity decreases with storage.

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Neurohistologic and Ultrastructural Lesions in Cattle Experimentally Intoxicated with the Plant Prosopis juliflora

Cited by 38 publications

References 4 publications

Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)

Autophagy protects against neural cell death induced by piperidine alkaloids present in Prosopis juliflora (Mesquite)

Intoxicação espontânea por vagens de Prosopis juliflora (Leg. Mimosoideae) em bovinos no Estado de Pernambuco

Efeitos teratogênicos de Prosopis juliflora em ratos e análise da toxicidade das vagens

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