Neuroimaging of a minipig model of Huntington's disease: Feasibility of volumetric, diffusion-weighted and spectroscopic assessments

Schubert, Robin; Frank, F; Nagelmann, Nina; Liebsch, L; Schuldenzucker, Verena; Schramke, Sarah; Wirsig, M; Johnson, Hans J.; Kim, Regina E. Y.; Ott, Sascha; Hölzner, E; Demokritov, S. O.; Motlı́k, Jan; Faber, Cornelius; Reilmann, Ralf

doi:10.1016/j.jneumeth.2015.11.017

Cited by 21 publications

(10 citation statements)

References 62 publications

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“…Humans and pigs share many common characteristics, including diet, body size, and brain size [1]. Pigs are believed to be the preferred disease model when compared with other species [2].…”

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confidence: 99%

Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

et al. 2019

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This study was carried out to examine the effects of manganese (Mn) on the developmental competence of porcine oocytes during in vitro maturation (IVM) after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT). Upon treatment of porcine oocytes with different concentrations (0, 3, 6, and 12 ng/ml) of Mn during IVM, PA was performed to determine the optimum concentration. Following PA, the rate of blastocyst formation was higher significantly in treated porcine oocytes at 6 ng/ml of Mn than in other groups (P < 0.05). However, there was no substantial difference in the cleavage rate and total blastocyst cell numbers among all groups. SCNT was performed using the optimal concentration of Mn from PA, which showed an improved blastocyst formation rate in treated oocytes compared to that in control group (P < 0.05). However, the cleavage rate and total cell numbers per blastocyst were not different between the control and the Mn treated groups after SCNT. Additionally, oocyte nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels were assessed. There was no significant difference observed in nuclear maturation among all the groups. However, enhanced intracellular GSH levels while lower levels of ROS were seen in the Mn treated group compared to the control group (P < 0.05). Thus, these results indicate that Mn supplementation can improve the developmental competence of porcine PA and SCNT embryos by increasing GSH and decreasing ROS levels.

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“…Humans and pigs share many common characteristics, including diet, body size, and brain size [1]. Pigs are believed to be the preferred disease model when compared with other species [2].…”

mentioning

confidence: 99%

Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

et al. 2019

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“…Indeed, extensive batteries that assess motor, cognitive, and behavioral functions as well as MRI imaging and spectroscopy have now been developed for tgHD minipigs. 90,91 Efforts are also under way in an attempt to generate tgHD or knock-in nonhuman primate models that could be used in HD preclinical studies. 92 It must be recognized, however, that although transgenic models are likely to be of greater value than toxic models in evaluating potential drugs for HD, they still do not precisely replicate the human disease, and their predictive value remains to be determined.…”

Section: The Role Of Preclinical Studiesmentioning

confidence: 99%

“…More recently transgenic models have been established in larger animals such as sheep and minipigs that may prove even more useful for preclinical studies. Indeed, extensive batteries that assess motor, cognitive, and behavioral functions as well as MRI imaging and spectroscopy have now been developed for tgHD minipigs . Efforts are also under way in an attempt to generate tgHD or knock‐in nonhuman primate models that could be used in HD preclinical studies .…”

Section: The Role Of Preclinical Studiesmentioning

confidence: 99%

Huntington's disease: Current and future therapeutic prospects

2018

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Huntington's disease is a progressive neurodegenerative disorder for which therapies are woefully inadequate and do not prevent inevitable progression. Currently approved therapies are primarily aimed at treating chorea, but do not address the more clinically meaningful motor, behavioral, and cognitive features of the disease. However, there are a number of promising new therapies that are currently being studied in the laboratory, and in the clinic. This article will review the wide variety of therapies currently being tested, the advances in clinical trials and end points, and the many potentially relevant new targets. © 2018 International Parkinson and Movement Disorder Society.

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“…Pigs share several key similarities with humans in terms of their body size, anatomical features, physiology, pathophysiological responses and diet, and are used to develop and refine biomedical procedures and medical equipment ( Heinritz et al, 2013 ; Kararli, 1995 ; Schubert et al, 2016 ). Their use in biomedical research is aided by several practical factors, such as their favourable breeding characteristics.…”

Section: Introductionmentioning

confidence: 99%

Genetically engineered pigs as models for human disease

Perleberg

Kind

Schnieke

2018

Disease Models &Amp; Mechanisms

177

136

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Genetically modified animals are vital for gaining a proper understanding of disease mechanisms. Mice have long been the mainstay of basic research into a wide variety of diseases but are not always the most suitable means of translating basic knowledge into clinical application. The shortcomings of rodent preclinical studies are widely recognised, and regulatory agencies around the world now require preclinical trial data from nonrodent species. Pigs are well suited to biomedical research, sharing many similarities with humans, including body size, anatomical features, physiology and pathophysiology, and they already play an important role in translational studies. This role is set to increase as advanced genetic techniques simplify the generation of pigs with precisely tailored modifications designed to replicate lesions responsible for human disease. This article provides an overview of the most promising and clinically relevant genetically modified porcine models of human disease for translational biomedical research, including cardiovascular diseases, cancers, diabetes mellitus, Alzheimer's disease, cystic fibrosis and Duchenne muscular dystrophy. We briefly summarise the technologies involved and consider the future impact of recent technical advances.

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Neuroimaging of a minipig model of Huntington's disease: Feasibility of volumetric, diffusion-weighted and spectroscopic assessments

Cited by 21 publications

References 62 publications

Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

Effects of manganese on maturation of porcine oocytes <i>in vitro</i> and their subsequent embryo development after parthenogenetic activation and somatic cell nuclear transfer

Huntington's disease: Current and future therapeutic prospects

Genetically engineered pigs as models for human disease

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