Neurological signs in pigs supplemented orally with pharmacological amounts of B vitamins from one to 10 months of age

Gutzwiller, A.; Bee, Giuseppe; Zimmermann, W.; Oevermann, Anna; Wyss-Fluehmann, G.; Raber, Martin N.; Geyer, Hans; Braunschweig, M

doi:10.1136/vr.165.20.598

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…The enriched diet was assumed to cause no harm to the F0 boars. After 4 months of age several E boars frequently showed symptoms of neurotoxicity [17]. It was hypothesized that the problems were caused by the high dosage of the potentially neurotoxic vitamin B 6 [17].…”

Section: Resultsmentioning

confidence: 99%

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Investigations on Transgenerational Epigenetic Response Down the Male Line in F2 Pigs

et al. 2012

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We investigated the nutritional effects on carcass traits, gene expression and DNA methylation in a three generation Large White pig feeding experiment. A group of experimental (E) F0 boars were fed a standard diet supplemented with high amounts of methylating micronutrients whereas a control group (C) of F0 boars received a standard diet. These differentially fed F0 boars sired F1 boars which then sired 60 F2 pigs. Carcass traits were compared between 36 F2 descendants of E F0 boars and 24 F2 descendants of C F0 boars. The two F2 offspring groups differed with respect to backfat percentage (P = 0.03) and tended to differ with respect to adipose tissue (P = 0.09), fat thickness at the 10th rib (P = 0.08) and at the croup (P = 0.09) as well as percentages of shoulder (P = 0.07). Offspring from the experimental F0 boars had a higher percentage of shoulder and were leaner compared to the control group. Gene expression profiles showed significant twofold differences in mRNA level between 8 C F2 offspring and 8 E F2 offspring for 79, 64 and 53 genes for muscle, liver and kidney RNA, respectively. We found that in liver and muscle respective pathways of lipid metabolism and metabolic pathway were over-represented for the differentially expressed genes between these groups. A DNA methylation analysis in promoters of differentially expressed genes indicated a significant difference in DNA methylation at the IYD gene. If these responses on carcass traits, gene expression and DNA methylation withstand verification and can indeed be attributed to transgenerational epigenetic inheritance, it would open up pioneering application in pork production and would have implications for human health.

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Section: Resultsmentioning

confidence: 99%

“…After 4 months of age several E boars frequently showed symptoms of neurotoxicity [17]. It was hypothesized that the problems were caused by the high dosage of the potentially neurotoxic vitamin B 6 [17]. In the following generations, pigs originating from the F0 E boars did not show any of the aforementioned signs.…”

Section: Resultsmentioning

confidence: 99%

Investigations on Transgenerational Epigenetic Response Down the Male Line in F2 Pigs

et al. 2012

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“…Exclusively the experimental F0 boars received high doses of methylating micronutrients intending to unbalance the one-carbon metabolism [14].…”

Section: Discussionmentioning

confidence: 99%

In Search of Epigenetic Marks in Testes and Sperm Cells of Differentially Fed Boars

2013

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In search of transmittable epigenetic marks we investigated gene expression in testes and sperm cells of differentially fed F0 boars from a three generation pig feeding experiment that showed phenotypic differences in the F2 generation. RNA samples from 8 testes of boars that received either a diet enriched in methylating micronutrients or a control diet were analyzed by microarray analysis. We found moderate differential expression between testes of differentially fed boars with a high FDR of 0.82 indicating that most of the differentially expressed genes were false positives. Nevertheless, we performed a pathway analysis and found disparate pathway maps of development_A2B receptor: action via G-protein alpha s, cell adhesion_Tight junctions and cell adhesion_Endothelial cell contacts by junctional mechanisms which show inconclusive relation to epigenetic inheritance. Four RNA samples from sperm cells of these differentially fed boars were analyzed by RNA-Seq methodology. We found no differential gene expression in sperm cells of the two groups (adjusted P-value>0.05). Nevertheless, we also explored gene expression in sperm by a pathway analysis showing that genes were enriched for the pathway maps of bacterial infections in cystic fibrosis (CF) airways, glycolysis and gluconeogenesis p.3 and cell cycle_Initiation of mitosis. Again, these pathway maps are miscellaneous without an obvious relationship to epigenetic inheritance. It is concluded that the methylating micronutrients moderately if at all affects RNA expression in testes of differentially fed boars. Furthermore, gene expression in sperm cells is not significantly affected by extensive supplementation of methylating micronutrients and thus RNA molecules could not be established as the epigenetic mark in this feeding experiment.

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Neurological signs in pigs supplemented orally with pharmacological amounts of B vitamins from one to 10 months of age

Cited by 2 publications

References 6 publications

Investigations on Transgenerational Epigenetic Response Down the Male Line in F2 Pigs

Investigations on Transgenerational Epigenetic Response Down the Male Line in F2 Pigs

In Search of Epigenetic Marks in Testes and Sperm Cells of Differentially Fed Boars

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