Neuron imaging with neurolucida — A PC-based system for image combining microscopy

Glaser, Jack R.; Glaser, Edmund M.

doi:10.1016/0895-6111(90)90105-k

Cited by 201 publications

(139 citation statements)

References 5 publications

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“…Data analysis includes lengths, surface areas, volumes, cell counts, etc. for the entire reconstruction (Glaser and Glaser, 1990). Another set of ovaries collected after saline perfusion was fixed overnight in Kahle's fixative and processed for 5-pm-thick paraf'fin sections.…”

Section: Methodsmentioning

confidence: 99%

The changing architecture of the neonatal rat ovary during histogenesis

Rajah¹,

Glaser²,

Hirshfield³

1992

Developmental Dynamics

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The purpose of this study was to describe the changing histological organization of the rat ovary during postpartum days one through three (pl-p3). A PC-based image-combining microscope system was used to reconstruct the ovary in three dimensions. On pl, cylindrical pocket-like structures radiated from the core of the ovary that were open toward the surface epithelium. The walls of the pockets contained connective tissue cells and capillaries (stroma). By p2, these pockets had completely closed; each pocket enclosed a small nest of oocytes and a few presumptive granulosa cells. By p3, the pocket-like organization had disappeared. On pl, only one or two primordial follicle-like structures were observed in the core and toward the periphery of the ovary; most oocytes were not enclosed in follicles. By p3, very few naked oocytes remained; primordial follicles predominated in all the regions of the ovary and some of the follicles had multiple layers of granulosa cells. There were changes in location, area, and volume of the rete tubules during these postnatal days. The extraovarian rete was visible on all 3 days but changed its orientation relative to the ovary. The connecting rete was found beneath the epithelial layer of the ovary on all 3 days and showed dramatic increase in area on p2. The wide lumen of the intraovarian rete was in direct contact with some of the oocytes near by on all 3 days, but these "communication points" were most abundant on p2. Based on our observations of different cell-cell associations during this time period, we hypothesize (1) that the mesenchymalpresumptive granulosa cell association is essential for the completion of folliculogenesis, and (2) the rete ovarii may have an inductive role in follicle assembly. These observations suggest that the first 3 days postpartum are critically important for studying the heterogeneous cell interactions that lead to the assembly of primordial follicles. The regional differences in tissue organization during this formative period may have significant implications on later aspects of follicular development. o 1992 Wiley-Liss, Inc.

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Section: Methodsmentioning

confidence: 99%

The changing architecture of the neonatal rat ovary during histogenesis

Rajah¹,

Glaser²,

Hirshfield³

1992

Developmental Dynamics

Self Cite

142

123

View full text Add to dashboard Cite

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“…Labeled cells were reconstructed from serial sections on a Nikon microscope with an apochromatic 100ϫ oil immersion objective equipped with a computer-interfaced motorized stage (Glaser and Glaser, 1990) and z-axis optical encoder using Neurolucida software (MicroBrightfield, Williston, VT). The external boundaries of the one or two spinal cord sections containing the soma were also traced (see Figs.…”

Section: Cell Reconstructionmentioning

confidence: 99%

Developmental changes in spinal motoneuron dendrites in neonatal mice

Brewer

Burke

et al. 2005

J of Comparative Neurology

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We examined the age-dependent morphological changes of lumbar spinal motoneurons (MNs) in neonatal Swiss-Webster mice during the first 2 weeks of postnatal life. Neurons labeled by intracellular injection of biocytin in hemisected lumbosacral spinal cords in vitro were reconstructed from serial sections. Digitized data were compared for young (P3; postnatal days 2-4; n ϭ 9) and older animals (P11; postnatal days 10 -13; n ϭ 8). As expected, measures of dendritic size (e.g., stem branch diameter, total surface area, maximum distance to tips, and lateral tree spread) were all significantly greater for P11 than for P3 mice. In contrast, the number of dendrites per MN and parameters related to tree topology (e.g., terminations per tree and maximum branch order), although slightly greater for P11 animals, were not significantly different between the two ages. Dendrite growth appeared to be proportional throughout the tree because the ratios between average terminal and internal branch lengths were similar for the two groups. Furthermore, this elongation was proportional to enlargement of overall spinal cord dimensions. A variety of other morphometric measures showed no significant difference between age groups. The relative constancy of MN dendritic topology up to P13 was surprising, given the striking maturation in motor function during this time period. J. Comp. Neurol. 483:304 -317, 2005 Dendrites are essential elements in understanding neural network connectivity and input/output properties of individual neurons in the central nervous system (see Stuart et al., 2001). Much of our current understanding of the linkage between dendritic morphology and the biophysics of electrotonic current flows within neuronal dendrites is based on early studies of spinal motoneurons (MNs) (Rall, 1959(Rall, , 1960(Rall, , 1977Burke and ten Bruggencate, 1971;Iansek and Redman, 1973;Jack et al., 1975). Aside from historical importance, the influence of MN dendrite morphology on the input/output relations of these cells is clearly relevant to understanding the control of motor unit recruitment. In addition, their involvement in amyotrophic lateral sclerosis (ALS) and other motor neuron diseases has generated considerable interest in the structural and functional development of mammalian MNs in fetal (Allan and Greer, 1997) and early postnatal life (Cameron and He, 1989;Curfs et al., 1993; Nuñ ez-Abades et al., 1993Nuñ ez-Abades and Cameron, 1995;Ramirez and Ulfhake, 1991;Walton and Navarrete, 1991; reviewed in Cameron and Nuñ ez-Abades, 2000).Because of the availability of spontaneous and genetically engineered neurological mutants, the mouse has become a key model species for studies of the influence of specific molecular pathways in the development and maintenance of neuronal dendrites (e.g

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“…rolucidae Microbrightfield, Inc. , based on the image-Ž combining microscope concept Glaser et al, 1983;Glaser . and Glaser, 1990 .…”

Section: Data Pre-processingmentioning

confidence: 99%

“…and Apkarian, 1992 . Furthermore, computer systems for axonal reconstruction and analysis have become available ŽCapowski, 1989;Glaser et al, 1983;Glaser and Glaser, . 1990 .…”

Section: Introductionmentioning

confidence: 99%