Neuronal cell differentiation of mesenchymal stem cells originating from canine amniotic fluid

Kim, Eun Young; Lee, Kyung-Bon; Yu, Jung Hwan; Lee, Ji Hye; Kim, Keun Jung; Han, Kil-Woo; Park, Kang–Sun; Lee, Dong‐Soo; Kim, Min Kyu

doi:10.1007/s13577-013-0080-9

Cited by 41 publications

(26 citation statements)

References 46 publications

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“…In the present study, the expressions of the neural stem cell marker Nestin as well as the neural precursor markers βIII-tubulin and NCAM were detected in canine OS cells. In previous studies [23,24,34] , these markers were also found in mesenchymal stem cells which were consistent with our findings. On the otherhand, we could not determine the GFAP mRNA expression in stem cells consisted with results of the study of Chung et al [24] .…”

Section: Discussionsupporting

confidence: 93%

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Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu

Altunbaş¹,

Yaprakci²,

Çeli̇k³

2015

Kafkas Univ Vet Fak Derg

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Olfactory stem cells have great potential in the treatment of neurodegenerative diseases and they are good candidates for cell therapy due to the easy accessibility of olfactory mucosa. The main objectives of this study were isolation, proliferation and characterization of olfactory mucosa stem cells that were further differentiated into olfactory neurospheres derived cells. When grown on poly-Dlysine with a serum-free culture medium supplemented with EGF (50 ng/ml) and FGF2 (50 ng/ml), olfactory stem cells gave rise to neurospheres. When grown in serum-containing culture medium newly plated spheres gave rise to olfactory neurosphere derived cells. Gene expression analysis revealed that, OCT4, SOX2, Nanog, Nestin, β-tubulin and NCAM were expressed in olfactory stem cells. While the mRNA expressions of Nanog, Nestin, Oct4, βIII-tubulin and NCAM were downregulated in neurospheres, the mRNA expression of SOX2 upregulated in neurospheres. According to the gene levels of neurospheres generated from olfactory stem cells, beta tubulin and NCAM gene expressions were upregulated, whereas OCT4, Nanog, Sox2 and Nestin mRNA expressions were downregulated in Olfactory neurospheres derived cells. Olfactory mucosa of canine is a suitable alternative source of stem cells and can be applied to cell therapy in neurodegenerative diseases. Keywords: Olfactory stem cell, Canine, Neurosphere, Pluripotent Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu ÖzetOlfaktorik kök hücreler nörodejeneratif hastalıkların tedavisinde büyük bir potansiyele sahiptir ve olfaktorik mukozaya kolay erişilebilirliği sayesinde hücre tedavisi için uygun bir adaydır. Bu çalışmanın amacı olfaktorik nörosfer kaynaklı hücrelere kadar farklılaştırılabilen olfaktorik kök hücrelerin izolasyonu, proliferasyonu ve karakterizasyonudur. Olfaktorik kök hücreler EGF (50 ng/ml) ve FGF (50 ng/ml) içeren serumsuz kültür vasatı içerisinde kültüre edildiğinde nörosferleri oluşturdular. Serum içeren kültür vasatında tekrar kültüre edildiklerinde olfaktorik nörosfer kaynaklı hücreleri şekillendirdiler. Gen ekspresyon analizleri OCT4, SOX2, Nanog, Nestin, β-tubulin ve NCAM genlerinin olfaktorik kök hücrelerinde eksprese olduğunu ortaya çıkardı. Nanog, Nestin, Oct4, β tubulin ve NCAM gen ekspresyonları nörosferlerde downregüle olurken, SOX2 geni upregüle oldu. Olfaktorik kök hücrelerin oluşturduğu nörosferlerin gen seviyeleri ile karşılaştırıldığında olfaktorik nörosfer kaynaklı hücrelerde β tubulin ve NCAM gen ekspresyonları upregüle olurken OCT4, Nanog, Sox2 and Nestin mRNA ekspresyonları downregüle oldu. Köpeğin olfaktorik mukozası uygun alternatif bir kök hücre kaynağıdır ve köpek nörodejeneratif hastalıklarında hücre tedavisi için uygulanabilir.

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Section: Discussionsupporting

confidence: 93%

“…The importance of the dog, being as a large animal model of human neurodegenerative diseases, has led to interest in the isolation and characterization of dog stem cells derived from various tissues such as adipose, bone marrow and amnion [22][23][24] .…”

Section: Introductionmentioning

confidence: 99%

Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu

Altunbaş¹,

Yaprakci²,

Çeli̇k³

2015

Kafkas Univ Vet Fak Derg

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“…44,74,75 Canine MSCs naturally express some of the neural markers 76 and show neuronal differentiation potentiality when isolated from various sources such as adipose tissue, 77 amniotic fluid, 78 and bone marrow. 79 CNTs have been studied as culture substrates to support neuron growth and differentiation basically due to their electrical conductivity as well as their nanotopographical feature creating tight contacts with neuron membranes, leading to electrical shortcuts.…”

Section: Das Et Almentioning

confidence: 99%

Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Das¹,

Madhusoodan²,

Mili³

et al. 2017

IJN

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In the field of regenerative medicine, numerous potential applications of mesenchymal stem cells (MSCs) can be envisaged, due to their ability to differentiate into a range of tissues on the basis of the substrate on which they grow. With the advances in nanotechnology, carbon nanotubes (CNTs) have been widely explored for use as cell culture substrate in tissue engineering applications. In this study, canine bone marrow-derived MSCs were considered as the cellular model for an in vitro study to elucidate the collective cellular processes, using three different varieties of thin films of functionalized carbon nanotubes (COOH-single-walled CNTs [SWCNTs], COOH-multiwalled CNTs [MWCNTs] and polyethylene glycol [PEG]-SWCNTs), which were spray dried onto preheated cover slips. Cells spread out better on the CNT films, resulting in higher cell surface area and occurrence of filopodia, with parallel orientation of stress fiber bundles. Canine MSCs proliferated at a slower rate on all types of CNT substrates compared to the control, but no decline in cell number was noticed during the study period. Expression of apoptosis-associated genes decreased on the CNT substrates as time progressed. On flow cytometry after AnnexinV-fluorescein isothiocyanate/propidium iodide (PI) staining, total number of apoptotic and necrotic cells remained lower in COOH-functionalized films compared to PEG-functionalized ones. Collectively, these results indicate that COOH-MWCNT substrate provided an environment of low cytotoxicity. Canine MSCs were further induced to differentiate along osteogenic, chondrogenic, and neuronal lineages by culturing under specific differentiation conditions. The cytochemical and immunocytochemical staining results, as well as the expression of the bone marker genes, led us to hypothesize that the COOH-MWCNT substrate acted as a better cue, accelerating the osteogenic differentiation process. However, while chondrogenesis was promoted by COOH-SWCNT, neuronal differentiation was promoted by both COOH-SWNCT and COOH-MWCNT. Taken together, these findings suggest that COOH-functionalized CNTs represent a promising scaffold component for future utilization in the selective differentiation of canine MSCs in regenerative medicine.

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“…Then many studies demonstrated that neural cells derived from bone marrow MSCs expressed mRNA species encoding many type of genes such as β-tubulin III (an early neural marker), nestin, neurofilaments (NFs), glial fibrillary acidic protein (GFAP) (as neural cell markers), and the specific neural markers such as choline acetyltransferase (ChAT), and MAP-2 and many other markers [22] [42] [44]- [46].…”

Section: Discussionmentioning

confidence: 99%

Production of Neural Progenitors from Bone Marrow Mesenchymal Stem Cells

Mohammad¹,

Yaseen²,

Al-Joubory³

et al. 2016

SCD

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In the brain, there are hundreds of types of specialized neurons and to generate one type of them we need to have neural progenitors for differentiation to specific neuron type. Mesenchymal stem cells (MSCs) are easily isolated, cultured, manipulated ex vivo, showing great potential for therapeutic applications. The adult MSCs have the potential to produce progeny that differentiate into a variety of cell types such as neurons. This fact suggests that MSCs derived neurons are an important cell type and a deep understanding of the molecular characteristics of it would significantly enhance the advancement of cell therapy for neurological disorders. Therefore, in this study, we isolated, identified, and studied neural progenitors by measuring expression levels through neurogenesis pathway of three neural differentiation markers nestin (NES), neurofilament (NF-L), and microtubule association protein (MAP-2) from mouse bone marrow MSCs (mouse bmMSCs) by using butylated hydroxyanisole (BHA) and diethyl sulfoxide (DMSO) as neural inducers agents. The results of immunocytochemistry and Real Time-PCR showed that in contrast to MSCs, neural differentiated cells showed neural progenitor pattern by showing stable increase in NES gene expression through differentiation process with increasing the protein expression through different exposures times, while NF-L gene and protein expression start to increased after 48 h but not replaced the NES expression completely even when its expression passed NES levels. The maturation marker Map-2 expression was low during the duration of differentiation period in protein and gene expression, which prove that these cells are still progenitors and can be redirected into specific type of neurons by further treatments. * Corresponding authors. M. Mohammad et al.2

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Neuronal cell differentiation of mesenchymal stem cells originating from canine amniotic fluid

Cited by 41 publications

References 46 publications

Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu

Köpek Olfaktorik Mukozasindan Olfaktorik Kök Hücrelerin Izolasyonu ve Karakterizasyonu

Functionalized carbon nanotubes as suitable scaffold materials for proliferation and differentiation of canine mesenchymal stem cells

Production of Neural Progenitors from Bone Marrow Mesenchymal Stem Cells

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