Neurons Containing Gastrin Releasing Peptide-Like Immunoreactivity in the Human Pancreas

Shimosegawa, Tooru; Asakura, Tadashi; Kashimura, Junya; Yoshida, Kazumasa; Meguro, Takayoshi; Koizumi, Masaru; Mochizuki, Tohru; Yanaihara, Noboru; Toyota, Takayoshi

doi:10.1097/00006676-199307000-00001

Cited by 21 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those nerves showed an increased expression of several neuropeptides (Buechler et al, 1992). Moreover, Shimosegawa et al (1993) discovered in chronic pancreatitis a frequent degeneration and fragmentation of nerve fibers displaying GRP-like immunoreactivity. The intrapancreatic nerve cell bodies, in contrast to those present in the healthy human pancreas, expressed a strong immunoreactivity for GRP, probably as a consequence of disturbed peptide transport, leading to an accumulation of GRP in the perikarya (Shimosegawa et al, 1993).…”

Section: Fleischmann Et Almentioning

confidence: 98%

“…Moreover, Shimosegawa et al (1993) discovered in chronic pancreatitis a frequent degeneration and fragmentation of nerve fibers displaying GRP-like immunoreactivity. The intrapancreatic nerve cell bodies, in contrast to those present in the healthy human pancreas, expressed a strong immunoreactivity for GRP, probably as a consequence of disturbed peptide transport, leading to an accumulation of GRP in the perikarya (Shimosegawa et al, 1993). It is conceivable, therefore, that the GRP receptors in the exocrine parenchyma of chronic pancreatitis will mediate abnormal neuronal information to the remaining exocrine glands, due to an altered GRP-mediated innervation.…”

Section: Fleischmann Et Almentioning

confidence: 99%

“…In the human pancreas, bombesin-like immunoreactivity has been localized by immunohistochemistry to nerve fibers innervating pancreatic acini, capillaries, ducts, and islets (Shimosegawa et al, 1993). Bombesin receptors have been identified with binding techniques under various experimental conditions in rat, guinea pig, and human pancreas tissues (Hajri et al, 1996;Huang et al, 1990;Jensen et al, 1978;Tang et al, 1997) indicative of a possible functional role of bombesin-like peptides in this organ (Deschodt-Lanckman et al, 1976).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Bombesin Receptors in Distinct Tissue Compartments of Human Pancreatic Diseases

Fleischmann

Läderach

Frieß

et al. 2000

Laboratory Investigation

View full text Add to dashboard Cite

SUMMARY: Overexpression of receptors for regulatory peptides in various human diseases is reportedly of clinical interest.Among these peptides, bombesin and gastrin-releasing peptide (GRP) have been shown to play a physiological and pathophysiological role in pancreatic tissues. Our aim has been to localize bombesin receptors in the human diseased pancreas to identify potential clinical applications of bombesin analogs in this tissue. The presence of bombesin receptor subtypes has been evaluated in specimens of human pancreatic tissues with chronic pancreatitis (n ϭ 23) and ductal pancreatic carcinoma (n ϭ 29) with in vitro receptor autoradiography on tissue sections incubated with 125 ]-bombesin(6 -14) as radioligands and displaced by subtype-selective bombesin receptor agonists and antagonists. GRP receptors were identified in the pancreatic exocrine parenchyma in 17 of 20 cases with chronic pancreatitis. No measurable bombesin receptors were found in the tumor tissue of ductal pancreatic carcinomas, however, GRP receptors were detected in a subset of peritumoral small veins in 19 of 29 samples. Moreover, residual pancreatic islets in these tissues were shown to express the BB3 receptor subtype. These data demonstrate the presence of bombesin receptors in three distinct tissue compartments of the pancreas, namely GRP receptors in the exocrine parenchyma in chronic pancreatitis and in peritumoral vessels around ductal pancreatic carcinomas, and BB3 receptors in residual pancreatic islets. Such a selective expression of bombesin receptor subtypes in pancreatic tissues may not only be of pathophysiological significance but may represent the basis for potential diagnostic and therapeutic clinical applications of bombesin analogs, including GRP receptor scintigraphy to differentiate chronic pancreatitis from ductal pancreatic carcinoma.

show abstract

Section: Fleischmann Et Almentioning

confidence: 98%

Section: Fleischmann Et Almentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Bombesin Receptors in Distinct Tissue Compartments of Human Pancreatic Diseases

Fleischmann

Läderach

Frieß

et al. 2000

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…Many studies have implicated the autonomic nervous system in islet function and glucose homeostasis (reviewed in (Ahrén, 2000; Brunicardi et al, 1995; Gilon and Henquin, 2001; Havel and Taborsky, 1989; Satin and Kinard, 1998; Woods and Porte, 1974). Few studies, however, have described the structural innervation of the human islet, and these studies are not comprehensive (Ahrén et al, 1991; Amenta et al, 1983; Bishop et al, 1980; Ding et al, 1997; Shimosegawa et al, 1993). Importantly, human islets have not been examined for the presence of classical markers of the parasympathetic and sympathetic systems.…”

Section: Introductionmentioning

confidence: 99%

Innervation Patterns of Autonomic Axons in the Human Endocrine Pancreas

et al. 2011

View full text Add to dashboard Cite

SUMMARY The autonomic nervous system regulates hormone secretion from the endocrine pancreas, the islets of Langerhans, and thus impacts glucose metabolism. The parasympathetic and sympathetic nerves innervate the pancreatic islet, but the precise innervation patterns are not known, particularly in human islets. Here we demonstrate that the innervation of human islets is different from that of mouse islets and that it does not conform to existing models of autonomic control of islet function. By visualizing axons in three dimensions and quantifying axonal densities and contacts within pancreatic islets, we found that, in contrast to mouse endocrine cells, human endocrine cells are sparsely contacted by autonomic axons. Few parasympathetic cholinergic axons penetrate the human islet and the invading sympathetic fibers preferentially innervate smooth muscle cells of blood vessels located within the islet. Thus, rather than modulating endocrine cell function directly, sympathetic nerves may regulate hormone secretion in human islets by controlling local blood flow or by acting on islet regions located downstream.

show abstract

“…One of these neurotransmitters may be gastrin releasing peptide (GRP) which is a neuropeptide localized to islet nerves (3)(4)(5) and released from the pancreas during activation of the vagus nerve (6). GRP stimulates both insulin and glucagon secretion under in vivo conditions and in isolated islets of Langerhans (4,(7)(8)(9)(10)(11) and the neuropeptide has been suggested to participate in the regulation of insulin release during meal intake (1).…”

mentioning

confidence: 99%