2016
DOI: 10.1002/eji.201546108
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Neurons exhibit Lyz2 promoter activity in vivo: Implications for using LysM‐Cre mice in myeloid cell research

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Cited by 95 publications
(83 citation statements)
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“…5E). It is worth noting that neuronal expression of Cre recombinase in LysM Cre mice has been reported by various groups including ours (Clausen et al, 2016; Orthgiess et al, 2016). However, since TNFR2 is virtually absent in CNS neurons (Brambilla et al, 2011; Zhang et al, 2014), neuronal Cre activity should not affect TNFR2 expression in LysM Cre : Tnfrsf1b fl/fl mice.…”
Section: Resultssupporting
confidence: 59%
“…5E). It is worth noting that neuronal expression of Cre recombinase in LysM Cre mice has been reported by various groups including ours (Clausen et al, 2016; Orthgiess et al, 2016). However, since TNFR2 is virtually absent in CNS neurons (Brambilla et al, 2011; Zhang et al, 2014), neuronal Cre activity should not affect TNFR2 expression in LysM Cre : Tnfrsf1b fl/fl mice.…”
Section: Resultssupporting
confidence: 59%
“…As always, each model has its limitations and particular intricacies the user needs be aware of for the design of the specific experimental set up and the question asked. The study of brain macrophages has been challenging, starting with the observation that the popular LysM Cre animals, that are often used for the study of peripheral myeloid cells, and were hence used to target microglia, show considerable rearrangements in neurons [33]. Here, we establish that also neither Cx 3 cr1 CreER nor Sall1 CreER transgenic mice specifically target microglia: Cx 3 cr1 CreER animals display in addition rearrangements in non-parenchymal CX 3 CR1 + CNS macrophages, while Sall1 CreER transgenic mice show prominent recombination in the neuro-ectodermal lineage.…”
Section: Discussionmentioning
confidence: 99%
“…Wt mice that received Tgfbr2 Myeko bone marrow exhibited mortality (Figure 1E) and brain microhemorrhages (Online Figure IF) similar to Tgfbr2 Myeko mice. These data exclude the contribution of brain residing microglia 10,11 and other cell types reported to be targets of LysM-cre 12 . Together, these data suggest that deficiency of TGFβ signaling in myeloid cells leads to decreased lifespan due to spontaneous stroke.…”
Section: Resultsmentioning
confidence: 64%