Pontine A5, A6 (locus coeruleus) and A7 cell groups provide noradrenergic innervation of the spinal cord. Here, we assessed whether activation of a 2-adrenoceptors in A7 influences peripheral nerve injury-induced hypersensitivity in the rat, and whether spinal a 2-adrenoceptors mediate the descending effect. Fadolmidine, an a 2-adrenoceptor agonist that because of its phar-macokinetic properties has a limited spread from the injection site, was microinjected through a chronic guide cannula into A7 or for comparison into A6 ipsilateral to the nerve injury. Moreover, atipamezole, an a 2-adrenoceptor antagonist, was injected intrathecally in an attempt to reverse the possible antihypersensitivity effect. Tactile hypersensitivity was assessed in the injured limb by determining limb withdrawal evoked by calibrated monofilaments, mechanical hyperalgesia by determining withdrawal evoked by noxious mechanical stimulation of the paw, and thermal nociception by assessing heat-induced withdrawal of the intact tail. Fadolmidine (1.0 or 3.0 lg) in A7, but not in A6, produced a tactile antihypersensitivity effect. Intrathecal atipamezole (5 lg) reversed the tactile antihypersensitivity effect by fadolmidine in A7. Atipamezole alone (5.0 lg) intrathecally or in A7 failed to influence tactile hypersensitivity. Fadolmidine in A7 failed to influence mechanical hyperalgesia or heat nociception at doses that produced a tactile antihypersensitivity effect. We propose that tonic noradrenergic drive of A6 by A7 promotes neuro-pathic hypersensitivity by suppressing descending noradrenergic inhibition originating in A6. Consequently, the activation of inhibitory a 2-adrenoceptors within the pontine A7 cell group suppresses neuropathic hypersensitivity by disinhibiting A6 and its descending noradrenergic pathways acting on spinal a 2-adrenoceptors. The noradrenergic cell groups A7, A6 (locus coeruleus) and A5 in the pons provide noradrenergic innervation of the spinal cord [e.g., 1]. These pontine noradrenergic cell groups are involved in descending modulation of pain through the action on spinal a 2-adrenoceptors. This is shown by studies in which chemical or electrical activation of A5, A6 or A7 produced antinociception that was reduced by spinal administration of a 2-adrenoceptor antagonists [for a review, see ref. 2]. A7 as well as other pontine noradrenergic nuclei has a high density of a 2-adrenoceptors [3,4]. a 2-Adrenoceptors, at least within A6, are to a large extent autoreceptors on noradrenergic neurons and their activation suppresses neuronal firing [5]. However, there is evidence indicating that heteroreceptors (i.e., a 2-adrenoceptors on non-adrenergic cells) play a significant role in presumably pontine as well as spinal a 2-adreno-ceptor functions [6]. Microinjections of a 2-adrenoceptor agonists into A6 of healthy animals have produced partly contradictory results that have varied from antinociception towards brief noxious stimuli [7] to hyperalgesia towards sustained noxious stimulation [8]. Peripheral nerve injury indu...