Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina

Vissani, M.A.; Becerra, M.L.; Perglione, Cecilia Olguín; Tordoya, M.S.; Miño, S.; Barrandeguy, M.

doi:10.1016/j.vetmic.2009.06.025

Cited by 48 publications

(47 citation statements)

References 10 publications

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“…This prevalence was lower than those observed in the U.S.A. (10.8-19.4%), Argentina (7.4%), France (24%), and Germany (10.6%) [11,23,24,26,28]. The samples that we analyzed were collected mainly from fetuses aborted in sporadic abortions or from outbreaks of non-neurological EHV-1.…”

Section: )mentioning

confidence: 64%

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Tsujimura

Oyama

Katayama

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. A single non-synonymous nucleotide substitution of guanine (G) for adenine (A) at position 2254 in the viral DNA polymerase gene (encoded by open reading frame [ORF] 30) of equine herpesvirus type 1 (EHV-1) has been significantly associated with neuropathogenic potential in strains of this virus. To estimate the prevalence of EHV-1 strains with the neuropathogenic genotype (ORF30 G 2254 ) in the Hidaka district-a major horse breeding area in Japan-we analyzed the ORF30 genomic region in cases of EHV-1 infection in this area during the years 2001-2010. Of the 113 cases analyzed, 3 (2.7%) were induced by ORF30 G 2254 strains. This prevalence is lower than those observed in the U.S

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Section: )mentioning

confidence: 64%

Prevalence of Equine Herpesvirus Type 1 Strains of Neuropathogenic Genotype in a Major Breeding Area of Japan

Tsujimura

Oyama

Katayama

et al. 2011

The Journal of Veterinary Medical Science

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“…Epidemiological studies have shown that a single-nucleotide polymorphism in the EHV-1 DNA polymerase gene is associated with outbreaks of severe neurological disease in horses. Reverse genetic experiments further demonstrated that a G 2254 A 2254 nucleotide mutation introduced in neurovirulent strain Ab4, which resulted in an asparagine for aspartic acid substitution (D 752 N 752 ), rendered the virus nonneurovirulent in the equine [A 2254 /752N -"non-neuropathogenic"; G 2254 /752D -"neuropathogenic"] (Allen 2007, Perkins et al 2009, Smith et al 2009, Vissani et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture

Cymerys

Dzieciątkowski²,

Słońska³

et al. 2010

Polish Journal of Veterinary Sciences

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Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus-neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.

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“…EHM was present in equine population as early as 1950s, however, its importance came to limelight in the last decade after large outbreaks of EHM occurred in Europe and America (Perkins et al, 2009;Vissani et al, 2009;Pronost et al, 2010;Smith et al, 2010;Fritsche & Borchers 2011;Pusterla et al, 2012;Damiani et al, 2014;Stasiak et al, 2015). Neurological disease can affect horses of all ages, including un-weaned foals, and often requires euthanasia of affected animal Horses exhibiting neurologic diseases can shed the virus in their nasal secretions and transmit the disease to in-contact animals (Henninger et al, 2007).…”

Section: Equine Herpesvirus Myeloencephalopathymentioning

confidence: 99%

“…At the same time, there has been rise in incidence of EHM in many parts of world viz., Europe, North America, South America, Africa and Oceania (Perkins et al, 2009;Vissani et al, 2009;Pronost et al, 2010;Smith et al, 2010;Fritsche & Borchers 2011;Tsujimura et al, 2011, Cuxson et al, 2014Negussie et al, 2015;McFadden et al, 2016). The neuropathogenic strains of EHV1 (causing EHM) have also been reported from Asian countries such as Japan (Tsujimura et al, 2011) and India (Unpublished data).…”

Section: Introductionmentioning

confidence: 99%