Neuropeptide B promotes proliferation and differentiation of rat brown primary preadipocytes

Abstract

Neuropeptide B (NPB) is reported to regulate energy homeostasis and metabolism via the NPBWR1 and NPBWR2 receptors in various tissues. However, the molecular mechanisms triggered from their interaction are not well investigated in brown adipose tissue. In this study, we specifically analyzed the role of NPB in controlling brown adipogenesis in rat brown preadipocytes. We first detected the expression of NPBWR1 and NPB on mRNA and protein level in brown preadipocytes and observed that NPB increased viability and proliferation of preadipocytes. Moreover, NPB stimulated expression of adipogenic genes (Prdm16, Ucp1) and suppressed the expression of antiadipogenic preadipocyte factor 1 (Pref1) during the differentiation process. Altogether, this led to an increase in intracellular lipid accumulation during preadipocyte differentiation, coupled with an increase in adrenaline‐induced oxygen consumption mediated by NPB. Furthermore, Ucp1 expression stimulated by NPB was attenuated by blockade of p38 kinase. In summary, we conclude that NPB promotes proliferation and differentiation of rat brown preadipocytes via p38‐dependent mechanism and plays an important role in controlling brown adipose tissue formation.

The Effects of Neuropeptide B on Proliferation and Differentiation of Porcine White Preadipocytes into Mature Adipocytes

“…Recently, we found that NPB is able to promote ERK1/2 phosphorylation in insulin-producing INS-1E cells [ 10 ]. Furthermore, we reported that NPB induces the phosphorylation of p38 kinase in rat white and brown primary preadipocytes [ 14 , 27 ]. As indicated in the introduction, both protein kinases, p-38 and ERK1/2, are involved in controlling adipogenesis [ 42 , 43 , 44 ], so we therefore studied whether NPB is able to modulate the phosphorylation of these protein kinases in porcine preadipocytes.…”

“…Recently, we provided evidence that NPB may be involved in controlling white and brown adipogenesis. Using rodent primary preadipocytes as well as the murine 3T3-L1 cell line, we found that NBP stimulates the proliferation and differentiation of brown and white preadipocytes [ 14 ]. These data suggest that NPB may be involved in controlling fat tissue formation in rodents.…”

The Role of Neuropeptide B and Its Receptors in Controlling Appetite, Metabolism, and Energy Homeostasis

“…In addition to the function of modulating white adipocytes, our recent data indicate that NPB may be involved in brown adipogenesis [ 28 ]. We demonstrated that NPB promotes proliferation of rat primary preadipocytes.…”