Neuropeptide W is expressed in the noradrenalin-containing cells in the rat adrenal medulla

Seki, Mayumi; Kageyama, Haruaki; Takenoya, Fumiko; Hirayama, Masami; Kintaka, Yuri; Inoue, Shuji; Matsuno, Ryosuke; Itabashi, Kazuo; Date, Yukari; Nakazato, Masamitsu; Shioda, Seiji

doi:10.1016/j.regpep.2007.08.011

Cited by 13 publications

(24 citation statements)

References 14 publications

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“…In addition, NPW mRNA is expressed in the urogenital system, including the kidney, testis, uterus, ovary, and placenta (Fujii et al, 2002). Based on RT-PCR analysis, we have confirmed the presence of NPW mRNA in the pituitary gland, adrenal gland, and stomach (Seki et al, 2008). These observations suggest that NPW may play an important role in the regulation of the endocrine system in response to stress, as well as in the activation of the hypothalamus-pituitary-adrenal (HPA) axis (Niimi and Murao, 2005; Seki et al, 2008).…”

Section: Distribution Of Npwmentioning

confidence: 58%

“…Rat adrenocortical cells are also shown to produce NPW (Hondo et al, 2008), as have noradrenalin-containing cells in the rat adrenal medulla (Seki et al, 2008) and gastric antral G cells in rats and mice (Mondal et al, 2006), with expression of NPW in the rat stomach mucosa being regulated by nutritional status, glucocorticoids, and thyroid hormones (Caminos et al, 2008). Hochol et al (2006) have reported expression of NPW in the thyroid and parathyroid glands, pancreatic islets, adrenal glands, ovary, and testis of the rat, while Rucinski et al (2007) have demonstrated NPW immunoreactivity in all of the cells of the pancreatic islets, including the A, B, and D cells, and PP cells.…”

Section: Distribution Of Npwmentioning

confidence: 99%

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Neuropeptide W

Takenoya¹,

Kageyama²,

Hirako³

et al. 2012

Front. Endocrin.

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Neuropeptide W (NPW), which was first isolated from the porcine hypothalamus, exists in two forms, consisting of 23 (NPW23) or 30 (NPW30) amino acids. These neuropeptides bind to one of two NPW receptors, either NPBWR1 (otherwise known as GPR7) or NPBWR2 (GPR8), which belong to the G protein-coupled receptor family. GPR7 is expressed in the brain and peripheral organs of both humans and rodents, whereas GPR8 is not found in rodents. GPR7 mRNA in rodents is widely expressed in several hypothalamic regions, including the paraventricular, supraoptic, ventromedial, dorsomedial, suprachiasmatic, and arcuate nuclei. These observations suggest that GPR7 plays a crucial role in the modulation of neuroendocrine function. The intracerebroventricular infusion of NPW has been shown to suppress food intake and body weight and to increase both heat production and body temperature, suggesting that NPW functions as an endogenous catabolic signaling molecule. Here we summarize our current understanding of the distribution and function of NPW in the brain.

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Section: Distribution Of Npwmentioning

confidence: 58%

Section: Distribution Of Npwmentioning

confidence: 99%

Neuropeptide W

Takenoya¹,

Kageyama²,

Hirako³

et al. 2012

Front. Endocrin.

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“…Moreover, icv administration of NPW stimulates plasma catecholamine concentrations in conscious rats and similar effects were observed at the adrenal medulla level (24,26,27). Thus, it cannot be excluded that in vivo both NPB and NPW may also affect bone cell activity acting via the central nervous system.…”

Section: Discussionmentioning

confidence: 91%

“…Those peptides and their receptors are also present in the neuroendocrine system and regulate its function. In this regard, involvement of both NPB and NPW in regulating hypothalamo-pituitaryadrenocortical axis is well recognised (17,(19)(20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells

Ziółkowska

Ruciński²,

Tyczewska³

et al. 2009

Int J Mol Med

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Abstract. Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar to NPBWR2 genes in cultured rat calvarial osteoblastlike (ROB) cells and the effects of both peptides on proliferative activity and osteocalcin secretion by ROB cells. Classic RT-PCR technique revealed the presence of ppNPB mRNA, ppNPW mRNA, and NPBWR1 mRNA, but not similar to NPBWR2 mRNA in ROB cells. QPCR revealed gradual (days 7, 14 and 21 of culture) increase of the ppNPB gene expression, while expression of ppNPW gene was the highest at day 14 and was comparable to that seen in freshly isolated cells. In ROB cells, expression of NPBWR1 gene was notable at day 7 of culture, lower at day 21, and negligible at day 14. Neither NPB nor NPW changed osteocalcin secretion by cultured osteoblast-like cells while both neuropeptides inhibited their proliferative activity. Results of the present study suggest that the systems of NPW, NPB and NPBWR1 directly regulate proliferative activity of cultured rat calvaria osteoblastlike cells. The physiological significance of this osteoblastic system remains unclear, and requires further investigation.

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“…Although the notion of NPW‐expressing neurons in the hypothalamus remains controversial, one plausible explanation for this discrepancy is due to different antibody sources, rather than to species‐specific differences. Differences in findings by in situ hybridization and immunohistochemistry may be due to the very low expression levels of NPW mRNA in the PVN, ARC, LH, and VMH, thus making it difficult to detect NPW mRNA without signal amplification such as the tyramide signal amplification (TSA) system 17 . Further studies using novel approaches and technologies are required to address the issue of the precise location of NPW‐containing neurons in the hypothalamus and other brain regions.…”

Section: Distribution and Localization Of Npw In The Brainmentioning

confidence: 99%

Neuropeptide W: a key player in the homeostatic regulation of feeding and energy metabolism?

Takenoya

Kageyama

Shiba

et al. 2010

Annals of the New York Academy of Sciences

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Neuropeptide W (NPW), recently isolated from porcine hypothalamus, has been identified as the endogenous ligand for both NPBWR1 (GPR7) and NPBWR2 (GPR8), which belong to the orphan G protein-coupled receptor family. NPW is thought to play an important role in the regulation of feeding and drinking behavior, and to be related to the stress response. NPW-containing neurons are localized in several regions of the brain, including the hypothalamus, hippocampus, limbic system, midbrain, and brain stem. Accumulated evidence suggests that hypothalamic neuropeptides, such as neuropeptide Y (NPY), orexin, melanin-concentrating hormone (MCH), and proopiomelanocortin (POMC), are involved in the regulation of feeding behavior and energy homeostasis via neuronal circuits in the hypothalamus. NPW also forms part of the feeding-regulating neuronal circuitry in conjunction with other feeding-regulating peptide-containing neurons within the hypothalamus. We summarize our current understanding of the distribution of NPW and of the neuronal interactions between NPW and the different feeding-regulating peptide-containing neurons. This review also discusses evidence for the dichotomous actions of NPW on energy balance and the potential mechanisms involved.

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Neuropeptide W is expressed in the noradrenalin-containing cells in the rat adrenal medulla

Cited by 13 publications

References 14 publications

Neuropeptide W

Neuropeptide W

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells

Neuropeptide W: a key player in the homeostatic regulation of feeding and energy metabolism?

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