Neuropeptides in Identified <i>Aplysia</i> Neurons

Kaldany, Rashad‐Rudolf J.; Nambu, John R.; Scheller, Richard H.

doi:10.1146/annurev.ne.08.030185.002243

Cited by 41 publications

(13 citation statements)

References 28 publications

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“…This could be due to Acp26Aa acting "downstream" of or independent of sex peptide. For example, by analogy to one of the functions of ELH in Aplysia (33), Acp26Aa might act directly on the musculature of the genital tract to stimulate contractions, resulting in rapid deposition of any matured eggs. However, we cannot rule out that transfer of sex peptide or another molecule that functions in receptivity could mask the possible involvement of Acp26Aa.…”

Section: Methodsmentioning

confidence: 99%

A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating.

Herndon

Wolfner

1995

Proc. Natl. Acad. Sci. U.S.A.

295

244

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Mating triggers behavioral and physiological changes in the Drosophila melanogaster female, including an elevation of egg laying. Seminal fluid molecules from the male accessory gland are responsible for initial behavioral changes, but persistence of these changes requires stored sperm. Using genetic analysis, we have identified a seminal fluid protein that is responsible for an initial elevation of egg laying. This molecule, Acp26Aa, has structural features of a prohormone and contains a region with amino acid similarity to the egg-laying hormone of Aplysia. Acp26Aa is transferred to the female during mating, where it undergoes processing. Here we report the generation and analysis of mutants, including a null, in Acp26Aa. Females mated to male flies that lack Acp26Aa lay fewer eggs than do mates of normal males. This effect is apparent only on the first day after mating. The null mutation has no other detectable physiological or behavioral effects on the male or the mated female.After mating, Drosophila melanogaster females undergo changes in their behavior and physiology. These changes persist for several days and include a stimulation of egg laying, a decrease in receptivity to further mating, and storage and utilization of sperm. In addition, mated females have a shorter life-span than unmated females (1, 2). D. melanogaster provides a genetic system to identify and examine the molecules that cause the changes in mated females. In this paper, we report that a molecule with sequence similarity to a hormone that stimulates egg laying in the mollusc Aplysia (3, 4) is involved in stimulating egg laying in D. melanogaster.Elevation of egg laying in Drosophila has two phases, a short-term phase that lasts 1 day and a long-term phase that lasts 7-9 days. Initially, egg laying is triggered by the transfer of seminal fluid to the female. This effect lasts I1 day (5). The long-term phase of increased egg laying requires the storage of sperm (5, 6). The seminal fluid components that stimulate egg laying derive from the accessory gland, a secretory tissue of the male reproductive tract. Transplantation of whole accessory glands into the abdomens of unmated females stimulated egg laying and decreased receptivity to mating to levels similar to those seen in mated females (7,8). Mates of male flies producing accessory gland secretions, but no sperm, show an increase in egg laying for 1 day only; mates of males lacking both accessory gland secretions and sperm show no stimulation (5).To identify products in accessory gland secretions, Chen et al. (9) assayed fractionated accessory gland extracts by injection into females. This process led to purification of a peptide (sex peptide) that stimulates egg laying for 1 day when injected in estimated physiological amounts into unmated females. Sex peptide can also decrease receptivity to mating for 1 day when introduced into unmated female flies (9). However, in at least one other Drosophila species in which a sex peptide exists, other molecules can also cause increased e...

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Section: Methodsmentioning

confidence: 99%

A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating.

Herndon

Wolfner

1995

Proc. Natl. Acad. Sci. U.S.A.

295

244

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“…However, msP 355a has an intriguing characteristic-its region of amino acid sequence similarity with ELH of A. californica. ELH is a 36-amino acid peptide hormone that is released from the bag cells of the abdominal ganglion as a result of mating (Kaldany et al 1985). ELH stimulates specific neurons of the abdominal, pedal, and buccal ganglia, thus contributing to the production of several stereotyped egg-laying behavior patterns (Scheller et al 1983).…”

Section: Towards the Function Of Msp 355a And Msp 355bmentioning

confidence: 99%

Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor.

Monsma

Wolfner²

1988

Genes Dev.

137

109

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The accessory gland of male insects is a genital tissue that secretes many components of the ejaculatory fluid, some of which affect the female's receptivity to courtship and her rate of oviposition. We have examined the structure and expression of two tightly linked genes that are expressed exclusively in the male accessory glands of adult Drosopbila melanogaster. The two genes are transcribed from the same strand of DNA, and are separated by 20 bases. Both genes are regulated by the sex determination hierarchy and are expressed in the absence of germ cells. Immunological analysis reveals the protein products of at least one of these genes in the secretion of the accessory gland. The proteins are transferred to the female fly during copulation and are rapidly altered in the female genital tract. The predicted sequence of one protein has features of a peptide hormone precursor, and a region in which 11 of 17 amino acids are identical to egg-laying hormone (ELH) of the California sea hare, Aplysia califomica.

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“…abundant. In addition to NTPs, the DCVs also contain high concentrations of Peptides A, B, and Califins (9). These peptides, however.…”

mentioning

confidence: 99%

Probing Single Secretory Vesicles with Capillary Electrophoresis

Chiu

Lillard

Scheller

et al. 1998

Science

Self Cite

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Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single vesicle that had attoliters (10(-18) liters) of volume was introduced into the tapered inlet of a separation capillary. Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2, 3-dicarboxaldehyde before separation. The resultant electropherograms indicated distinct variations in the contents of single vesicles.

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Neuropeptides in Identified Aplysia Neurons

Cited by 41 publications

References 28 publications

A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating.

A Drosophila seminal fluid protein, Acp26Aa, stimulates egg laying in females for 1 day after mating.

Structure and expression of a Drosophila male accessory gland gene whose product resembles a peptide pheromone precursor.

Probing Single Secretory Vesicles with Capillary Electrophoresis

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