Neuroprotection against CCl
            <sub>4</sub>
            induced brain damage with crocin in Wistar rats

Altınöz, Eyüp; Erdemli, Mehmet Erman; Gül, Mehmet; Aksungur, Zeynep; Gül, Semir; Bağ, Harika Gözükara; Kaya, Gülşen; Türköz, Yusuf

doi:10.1080/10520295.2018.1519725

Cited by 19 publications

(12 citation statements)

References 65 publications

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“…In the present research, it was revealed that the expression of UCA1 was significantly upregulated in DLBCL tissues and cell lines. Consistent with our results, UCA1 was also upregulated in gastric (22) and adrenocortical cancer (23). In glioma, UCA1 was detected to drive cell proliferation, migration and EMT (24).…”

Section: Mir-331-3p Expression --------------------------------------supporting

confidence: 91%

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Zhang

Shang

et al. 2020

Oncol Lett

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Long non-coding RNA urothelial cancer associated 1 (UCA1) has been reported to act as a carcinogen in bladder cancer, while its role in diffuse large B-cell lymphoma (DLBCL) remains unclear. The present study was designed to explore the expression pattern and role of UCA1 in DLBCL. The expression pattern of UCA1 and microRNA (miR)-331-3p in DLBCL tissues and cell lines were detected by RT-qPCR. Dual luciferase reporter assay was performed to explore the relationship between UCA1 and miR-331-3p. Cell proliferation was explored by MTT assay. Cell migration and invasion abilities were assessed by Transwell assay. In the present study, it was revealed that the expression of UCA1 was significantly upregulated, while miR-331-3p was downregulated in DLBCL tissues and cell lines. Moreover, UCA1 was revealed to competitively bind with miR-331-3p in DLBCL. Functionally, knockdown of UCA1 was revealed to suppress cell proliferation, migration and invasion in DLBCL cells. Furthermore, upregulation of miR-331-3p prevented cell proliferation, migration and invasion in DLBC cells. In conclusion, the present findings firstly demonstrated that UCA1 silencing restrained DLBCL cell proliferation and metastases viability by suppressing miR-331-3p expression. It is suggested that UCA1 could be a possible medicinal target and biomarker for DLBCL.

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Section: Mir-331-3p Expression --------------------------------------supporting

confidence: 91%

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Zhang

Shang

et al. 2020

Oncol Lett

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“…This suggests that nesting activity is either the most sensitive method to detect distress or CCl 4 causes a specific change in nesting behavior independent of inducing distress. Consistent with the second hypothesis is the observation that CCl 4 can change protein expression, reduce the number of neurons in the brain [ 46 ] and can also lead to brain damage [ 47 ]. Such changes in the central nervous system could influence complex behavior such as nesting activity.…”

Section: Discussionmentioning

confidence: 83%

Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods

Zechner

Schulz

Tang

et al. 2022

Animals

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An essential basis for objectively improving the status of animals during in vivo research is the ability to measure the wellbeing of animals in a reliable and scientific manner. Several non-invasive methods such as assessing body weight, burrowing activity, nesting behavior, a distress score and fecal corticosterone metabolites were evaluated in healthy mice and after three surgical interventions or during the progression of four gastrointestinal diseases. The performance of each method in differentiating between healthy and diseased animals was assessed using receiver operating characteristic curves. The ability to differentiate between these two states differed between distinct surgical interventions and distinct gastrointestinal diseases. Thus, the generalizability of these methods for assessing animal wellbeing was low. However, the robustness of these methods when assessing wellbeing in one gastrointestinal disease was high since the same methods were often capable of differentiating between healthy and diseased animals independent of applied drugs. Moreover, the replicability when assessing two distinct cohorts with an identical surgical intervention was also high. These data suggest that scientists can reach valid conclusions about animal wellbeing when using these methods within one specific animal model. This might be important when optimizing methodological aspects for improving animal wellbeing. The lack of generalizability, however, suggests that comparing animal models by using single methods might lead to incorrect conclusions. Thus, these data support the concept of using a combination of several methods when assessing animal welfare.

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“… 37 , 93 and these have strong antioxidant properties. 94 , 95 Therefore, the herb can be utilized in the development of therapeutic molecules 47 , 96 - 100 due to the neuroprotective attributes of flavonoids, polyphenols, and chromones that have been demonstrated in brain cells of rodents 37 - 39 via antioxidant and receptor inhibition mechanisms 37 - 39 and these mechanisms are linked to antiseizure, antiepileptogenic, and cognitive deficit alleviation attributes in rodents with neurological disorders. 40 , 41 …”

Section: Discussionmentioning

confidence: 99%

“… 34 - 36 Phytochemicals from the herb such as the 2-(2-Phenylethyl) and 5-Hydroxy-2-(2-phenylethyl) chromones have been found to possess neuroprotective potential on glutamate-induced neurotoxicity in rat cerebral cortical cells, 37 , 38 additionally plant-based nonenzymatic antioxidants mainly phenols, flavonoids, chromones, and glycosides provide neuroprotective attributes against brain cell damage through their strong antioxidant potentials that improve brain cell biology, 37 , 39 provide anticonvulsant and antiepileptogenic attributes, 40 and alleviate cognitive deficits 41 of animals by the antioxidation mechanism which controls oxidative stress markers in the brain of animals. 39 - 41 …”

Section: Introductionmentioning

confidence: 99%

Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models

Ssempijja

Dare

Bukenya

et al. 2023

J Evid Based Complementary Altern Med

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Imperata cylindrica is a globally distributed plant known for its antiepileptic attributes, but there is a scarcity of robust evidence for its efficacy. The study investigated neuroprotective attributes of Imperata cylindrica root extract on neuropathological features of epilepsy in a Drosophila melanogaster mutant model of epilepsy. It was conducted on 10-day-old (at the initiation of study) male post-eclosion bang-senseless paralytic Drosophila ( parabss1) involved acute (1-3 h) and chronic (6-18 days) experiments; n = 50 flies per group (convulsions tests); n = 100 flies per group (learning/memory tests and histological examination). Administrations were done in 1 g standard fly food, per os. The mutant flies of study ( parabss1) showed marked age-dependent progressive brain neurodegeneration and axonal degeneration, significant (P < 0.05) bang sensitivity and convulsions, and cognitive deficits due to up-regulation of the paralytic gene in our mutants. The neuropathological findings were significantly (P < 0.05) alleviated in dose and duration-dependent fashions to near normal/normal after acute and chronic treatment with extract similar to sodium valproate. Therefore, para is expressed in neurons of brain tissues in our mutant flies to bring about epilepsy phenotypes and behaviors of the current juvenile and old-adult mutant D. melanogaster models of epilepsy. The herb exerts neuroprotection by anticonvulsant and antiepileptogenic mechanisms in mutant D. melanogaster due to plant flavonoids, polyphenols, and chromones (1 and 2) which exert antioxidative and receptor or voltage-gated sodium ion channels’ inhibitory properties, and thus causing reduced inflammation and apoptosis, increased tissue repair, and improved cell biology in the brain of mutant flies. The methanol root extract provides anticonvulsant and antiepileptogenic medicinal values which protect epileptic D. melanogaster. Therefore, the herb should be advanced for more experimental and clinical studies to confirm its efficacy in treating epilepsy.

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Neuroprotection against CCl ₄ induced brain damage with crocin in Wistar rats

Cited by 19 publications

References 65 publications

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods

Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models

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Neuroprotection against CCl 4 induced brain damage with crocin in Wistar rats

Cited by 19 publications

References 65 publications

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Knockdown of UCA1 restrains cell proliferation and metastasis of diffuse large B‑cell lymphoma by counteracting miR‑331‑3p expression

Generalizability, Robustness and Replicability When Evaluating Wellbeing of Laboratory Mice with Various Methods

Attenuation of Seizures, Cognitive Deficits, and Brain Histopathology by Phytochemicals of Imperata cylindrica (L.) P. Beauv (Poaceae) in Acute and Chronic Mutant Drosophila melanogaster Epilepsy Models

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Neuroprotection against CCl ₄ induced brain damage with crocin in Wistar rats