Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic–Ischemic Brain Injury

Jiao, Mengya; Dong, Qun; Zhang, Yiting; Lin, Min Jon; Zhou, Wan; Liu, Tao; Yuan, Baohong; Yin, Hui

doi:10.3390/molecules26113108

Cited by 7 publications

(2 citation statements)

References 52 publications

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“…TTC staining was performed 1 day after ischemia/reperfusion (46,47). Freshly cut 1-mm-thick brain sections were stained with 2% 2,3,5-triphenyltetrazolium chloride (TTC, Sigma-Aldrich, USA) for 15 min at 37 • C while protected from light, and then fixed in 4% PFA overnight and photographed.…”

Section: Histological Stainingmentioning

confidence: 99%

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

Wang

et al. 2022

Front. Neurol.

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Diabetes is one of the well-established risk factors of stroke and is associated with a poor outcome in patients with stroke. Previous studies have shown that the expression of neuron restrictive silencer factor (NRSF) is elevated in diabetes as well as ischemic stroke. However, the role of NRSF in regulating an outcome of diabetic ischemic stroke has not been completely understood. Here, we hypothesized that diabetes-induced NRSF elevation can aggravate brain injury and cognition impairment in ischemic stroke. The diabetic ischemic stroke mice model was established by 8 weeks of high-fat-diet feeding and 5 days of streptozotocin injection followed by 30 min of middle cerebral artery occlusion (MCAO). We found that diabetes enhanced the MCAO-induced elevation of NRSF in the hippocampus in accompany with an elevation of its corepressors, HDAC1, and mSin3A, and decrease of β-TrCP. By using histological/immunofluorescence staining and neurobehavioral testing, our results showed that the brain damage and learning/memory impairment were aggravated in diabetic ischemic mice but significantly attenuated after stereotaxic injection of NRSF-shRNA. Meanwhile, by performing whole-brain clearing with PEGASOS, microvascular reconstruction, western blotting, and ELISA, we found that NRSF-shRNA markedly alleviated the vasculature disorders and rescued the suppression of NRP-1, VEGF, and VEGFR2 in the hippocampus of diabetic ischemic mice. Therefore, our results demonstrated for the first time that the elevation of hippocampal NRSF plays an important role in alleviating brain injury and cognitive disabilities in diabetic ischemic mice, potentially via the reduction of NRP-1/VEGF signaling.

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Section: Histological Stainingmentioning

confidence: 99%

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

Wang

et al. 2022

Front. Neurol.

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“…Macamides are considered functional components of the maca plant, unique to this species. Research in recent years has shown that macamides have a variety of pharmacological activities, including a vital effect on endurance capacity [10], neuroprotection [10,11], colitis [12], ovarian injury [13], and testicular function and spermatogenesis [14]. Moreover, some macamides act as irreversible inhibitors of fatty acid amide hydrolases (FAAH) and have…”

Section: Introductionmentioning

confidence: 99%

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

et al. 2023

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Macamides are a class of amide alkaloids that are only found in maca and are widely considered to be its bioactive marker compounds. More than thirty macamide monomers have been identified in recent years; however, it is difficult to obtain a single macamide monomer from the maca plant because of their similar structures and characteristics. We used the carbodiimide condensation method (CCM) to efficiently synthesize five typical macamides, including N-benzyl-hexadecanamide (NBH), N-benzyl-9Z,12Z,15Z-octadecenamide, N-(3-methoxybenzyl)-9Z,12Z-octadecenamide, N-benzyl-9Z,12Z-octadecenamide, and N-(3-methoxybenzyl)-9Z,12Z,15Z-octadecadienamide. All the synthesized macamides were purified by a one-step HPLC with a purity of more than 95%. NBH is the most abundant macamide monomer in natural maca, and it was selected to evaluate the anti-fatigue effects of macamides. The results indicated that NBH could enhance the endurance capacity of mice by increasing liver glycogen levels and decreasing blood urea nitrogen, lactate dehydrogenase, blood ammonia, and blood lactic acid levels. Macamides might be the active substances that give maca its anti-fatigue active function.

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Bioactive Compounds and Biological Activities of Peruvian Maca (Lepidium meyenii Walp.)

Carvalho,

da Silva,

Ribeiro

2023

Reference Series in Phytochemistry

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Neuroprotection of N-benzyl Eicosapentaenamide in Neonatal Mice Following Hypoxic–Ischemic Brain Injury

Cited by 7 publications

References 52 publications

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice

The Efficient Synthesis and Anti-Fatigue Activity Evaluation of Macamides: The Unique Bioactive Compounds in Maca

Bioactive Compounds and Biological Activities of Peruvian Maca (Lepidium meyenii Walp.)

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