Neuroprotective Activity of Novel Cur-Ca-Thione and Its Oxidative Stress Study

Abstract

Objective: Alzheimer's disease a progressive neurodegenerative disorder affected by the formation of amyloid beta and tau proteins. Medicinal plants have been proved significantly for its anti-oxidant and anti-inflammatory activity that might help in treating neurological disorders. Curcumin has been hugely studied in the treatment of various ailments but its water solubility and bioavailability is still a concern but we have tried to exterminate the problem by our formulation CUR-CA-THIONE. Now, we have expanded the study of CUR-CA-THIONE for its neuroprotective estimation by evaluating behavioral, biochemical and histopathological assessment in rats.Methods: Wistar rats of either sex (M/F: 25-350g; 350-450 g) were selected for study and divided into 8 groups. All animals except NC were given aluminum chloride via oral route throughout the study period (30 d) while group 3-8 received treatment one-hour post aluminum chloride induction from 15 th day to 30 th day. One week prior to the start of the experiment all animals were given training for behavioral assessment through y-maze and morris water maze with the weekly assessment. On 30 th Results:The results of the behavioral assessment in CUR-CA-THIONE complex showed an increase in total arm entries in Y-maze and decrease in time duration for morris water maze test. A significant (p<0.01) decrease in lipid peroxidation, superoxide dismutase, acetylcholine and total protein levels in formulations while significant (p<0.01) increase in glutathione and catalase level was observed.-day post completion of study rat brain was isolated to study biochemical and histopathological examination. Conclusion:The given formulation shows that curcumin-casein-glutathione complex shows potential action as a neuroprotective effect.

A Mechanistic Evaluation of Antioxidant Nutraceuticals on Their Potential against Age-Associated Neurodegenerative Diseases

“…Nevertheless, modification of curcumin by conjugating it with a carrier, encapsulation within the nanoparticle, and modification of its chemical structure have rendered positive results, including enhanced activity towards cancer cells, better wound healing properties, and strong antibacterial effects against Pseudomonas aeruginosa and Staphylococcus aures [ 89 , 90 ]. Shelat et al [ 91 ] reported that treatment with oral CUR-CA-THIONE, an alternative formulation derived from curcumin using glutathione and casein as vectors for better water solubility and brain bioavailability [ 89 ], at a dose of 500 mg/kg BW/day for 15 days to the aluminum chloride (AlCl 3 )-induced AD Wistar rats successfully inhibited LPO formation as measured by a thiobarbituric acid reactive substances (TBARS) assay ( p < 0.05) compared to untreated AD rats, which was associated with improved locomotor and exploratory behavior. Moreover, in an in vitro study, a curcumin derivative, demethoxycurcumin, reduced intracellular ROS generation induced by neurotoxic rotenone [ 92 ].…”

Morris Water Maze – A Bench Mark Test for Learning and Memory Disorders in Animal Models: A Review

“…In the contest of the use of this test, it might be emphasized that there are also other modifications and protocols including a working memory version, cognitive flexibility, neuroprotection, cholinergic dysfunction, and path integration in the MWM [11][12][13][14][15][16]. Testing the working memory in MWM is required due to information processing and working memory deficits in many central nervous system disorders, such as Schizophrenia, Parkinson, and Alzheimer's [17].…”