Objective: Curcumin, is widely studied as a potential drug in treating various disorders but lacks applicability due to poor water solubility and tissue bioavailability. The main objective of the study was to develop a formulation of curcumin that has enhanced water solubility and brain bioavailability.Methods: A curcumin concoction was prepared using solvent evaporation technique taking casein and glutathione as vectors. Various process parameters were identified namely time, temperature, pH and vector while formulation parameters included drug entrapment, anti-oxidant activity, and water solubility. The concoctions were evaluated for in vitro release kinetics at three pH i.e. 1.2, 4.5 and 6.2 at six-time intervals i.e. 10, 20, 30, 40, 60, 120 min using dialysis bag membrane. The same kinetics was further validated using same time points with wistar rats and giving concoction at a single dose of 2 g/kg via the oral route.Results: A concoction i.e. CUR-CA-THIONE having significant entrapment efficiency (77.83%, 97.75%, 90.19%), water solubility (40, 350 and 45 times than normal curcumin) and DPPH activity (IC50 Conclusion:The experiment helps in concluding that CUR-CA-THIONE has improved its water solubility and is able to by-pass systemic circulation to targeted activity.: 28.91, 25.07 and 27.89) was evaluated in concoctions CUR-CA-THIONE-T.1, CUR-CA-THIONE-T.2 and CUR-CA-THIONE-T.3 respectively. These formulations were then carried out for in vitro release profile at different pH with average release obtained between 20-30 min. In vivo kinetics was studied by isolating tissues like brain, liver, lung, kidney and spleen in male wistar rats and maximum brain bioavailability was observed for CUR-CA-THIONE-T.3 at 30 min with 75 ng/g of brain tissue.

Objective: Alzheimer's disease a progressive neurodegenerative disorder affected by the formation of amyloid beta and tau proteins. Medicinal plants have been proved significantly for its anti-oxidant and anti-inflammatory activity that might help in treating neurological disorders. Curcumin has been hugely studied in the treatment of various ailments but its water solubility and bioavailability is still a concern but we have tried to exterminate the problem by our formulation CUR-CA-THIONE. Now, we have expanded the study of CUR-CA-THIONE for its neuroprotective estimation by evaluating behavioral, biochemical and histopathological assessment in rats.Methods: Wistar rats of either sex (M/F: 25-350g; 350-450 g) were selected for study and divided into 8 groups. All animals except NC were given aluminum chloride via oral route throughout the study period (30 d) while group 3-8 received treatment one-hour post aluminum chloride induction from 15 th day to 30 th day. One week prior to the start of the experiment all animals were given training for behavioral assessment through y-maze and morris water maze with the weekly assessment. On 30 th Results:The results of the behavioral assessment in CUR-CA-THIONE complex showed an increase in total arm entries in Y-maze and decrease in time duration for morris water maze test. A significant (p<0.01) decrease in lipid peroxidation, superoxide dismutase, acetylcholine and total protein levels in formulations while significant (p<0.01) increase in glutathione and catalase level was observed.-day post completion of study rat brain was isolated to study biochemical and histopathological examination. Conclusion:The given formulation shows that curcumin-casein-glutathione complex shows potential action as a neuroprotective effect.