Neuroprotective and cognitive-enhancing effects of the combined extract of Cyperus rotundus and Zingiber officinale

Sutalangka, Chatchada; Wattanathorn, Jintanaporn

doi:10.1186/s12906-017-1632-4

Cited by 51 publications

(31 citation statements)

References 41 publications

(27 reference statements)

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“…a-Cyperone, extracted from Cyperus rotundus, has been demonstrated to weaken the inflammatory response by microtubule destabilization in the brain (Azimi et al, 2016). Sutalangka et al has reported Cyperus rotundus has neuroprotective and cognitive-enhancing effects in AF64A-treated rats (Sutalangka and Wattanathorn, 2017). We previously showed that a-cyperone exerts neuroprotective effects by inhibiting microglia-mediated neuroinflammation (Huang et al, 2018).…”

Section: Introductionmentioning

confidence: 87%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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a-Cyperone, extracted from Cyperus rotundus, has been reported to inhibit microgliamediated neuroinflammation. Oxidative stress and apoptosis play crucial roles in the course of Parkinson's disease (PD). PD is a common neurodegenerative disease characterized by selective death of dopaminergic neurons. This study was designed to investigate the neuroprotective effects of a-cyperone against hydrogen peroxide (H 2 O 2)induced oxidative stress and apoptosis in dopaminergic neuronal SH-SY5Y cells. Neurotoxicity was assessed by MTT assay and the measurement of lactic dehydrogenase (LDH) release. The level of reactive oxygen species (ROS) was measured by dichlorodihydrofluorescin diacetate (DCFH-DA) staining. The apoptosis of SH-SY5Y cells was evaluated by annexin-V-FITC staining. The translocation of NF-E2related factor 2 (Nrf2) was determined by western blot and immunofluorescence staining. Western blot analysis was conducted to determine the expression level of cleavedcaspase-3, the pro-apoptotic factor Bax, and the anti-apoptotic factor, Bcl-2. The results showed that a-cyperone substantially decreased H 2 O 2-induced death, release of LDH, and the production of ROS in SH-SY5Y cells. In addition, we found that a-cyperone attenuated H 2 O 2-induced cellular apoptosis. Moreover, a-cyperone remarkably reduced the expression of cleaved-caspase-3 and Bax, and upregulated Bcl-2. Furthermore, acyperone enhanced the nuclear translocation of Nrf2. Pretreatment with brusatol (BT, an Nrf2 inhibitor) attenuated a-cyperone-mediated suppression of ROS, cleaved-caspase-3, and Bax, as well as a-cyperone-induced Bcl-2 upregulation in H 2 O 2-treated SH-SY5Y cells. a-cyperone neuroprotection required Nrf2 activation. In conclusion, a-cyperone attenuated H 2 O 2-induced oxidative stress and apoptosis in SH-SY5Y cells via the activation of Nrf2, suggesting the potential of this compound in the prevention and treatment of PD.

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Section: Introductionmentioning

confidence: 87%

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Huang

Liu

et al. 2020

Front. Pharmacol.

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“…The results of the in vitro AChE inhibition studies performed by other authors have been confirmed by several in vivo assays, that determine the actual activity of ginger extracts or fractions in living organisms. Sutalangka and Wattanathorn [1] observed in their study on rats that after an oral administration of Zingiber officinale and Cyperus rotundus extracts the cognition and memorizing processes were elevated and AChE activity decreased. These extracts were found to affect the cholinergic functions of brain and decrease neurodegeneration and oxidative stress.…”

Section: Introductionmentioning

confidence: 97%

“…The currently available medications can slow down the progression of dementia, but not to treat it. Based on the above information, the search for new potential drug candidates that retard the progression of memory impairment is of the highest importance [1,2]. Among the mechanisms of dementia are disturbances in acetylcholine synthesis and an overproduction of esterases, that decompose this neuromodulator, have been highlighted in various scientific reports.…”

Section: Introductionmentioning

confidence: 99%

Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes—Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies

et al. 2020

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Although numerous studies have been conducted on ginger extracts and fractions, the data on the pharmacological activity of single constituents of Zingiber officinale are still insufficient. To assess the antidementia properties of the plant, a thin layer chromatography (TLC)-based bioautography acetylcholinesterase inhibitory assay was performed on the Zingiber officinale diethyl ether extract. It led to the recognition of three active inhibitors among volatile constituents of the plant: ar-curcumene (A), α-sesquiphellandrene (B) and a-zingiberene (C). The identification of the components was possible thanks to the application of a TLC–HPLC-MS interface analysis of active zones and the GC-MS qualitative analysis of the tested samples. Based on the obtained results, the influence of several extraction techniques (hydrodistillation—HD, pressurized liquid extraction or accelerated solvent extraction—ASE, shaking maceration–SM, supercritical fluid extraction–SFE, and ultrasound-assisted extraction—UAE) on the recovery of the active metabolites from plant material was assessed to deliver enriched extracts. As a result, HD and SFE, were found to be the most efficient methods to recover the volatile components and the concentrations of A, B, and C reached 0.51 ± 0.025, 0.77 ± 0.045, and 1.67 ± 0.11 percent, respectively. Only HD and SFE were found to recover monoterpene hydrocarbons from the plant matrix. The remaining techniques provided extracts rich in more complex constituents, like sesquiterpenes.

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“…In experimental hypothyroidism, thyroid hormones dysfunction has been reported to directly relate to ACh deficiency in particular parts of the brain including hippocampus and basal forebrain (Patel, Hayashi, & Hunt, 1987;Patel, Smith, Kingsbury, Hunt, & Balazs, 1980;Smith et al, 2002). On the contrary, all herbs used in the current study, including C. sativus (Geromichalos et al, 2012), C. rotundus (Sutalangka & Wattanathorn, 2017), B. serrate (Yassin et al, 2013), and P. nigrum (Ingkaninan, Temkitthawon, Chuenchom, Yuyaem, & Thongnoi, 2003) have been reported to increase ACh content in the brain through reducing the AChE activity.…”

Section: Central Cholinergic Transmission Deficiency Is An Importantmentioning

confidence: 56%

“…rotundus is a potent antioxidant and acetylcholinesterase (AChE) inhibitor, which is reported to exert neuroprotective and memory-enhancing effects (Mehdizadeh et al, 2017;Sutalangka & Wattanathorn, 2017). C. sativus (saffron) traditionally used as a food additive and a medical plant (Akhondzadeh et al, 2010;Pitsikas et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

The effects of the combination ofCyperus rotundus,Crocus sativus,Piper nigrum,andBoswellia serrataon learning and memory deficit and oxidative damage in brain tissue of hypothyroid rats

et al. 2020

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In the present study, the impact of a combination of four memory‐enhancer herbs on cognitive impairment and brain tissue oxidative damage due to hypothyroidism was evaluated. Propylthiouracil (PTU; 0.05%) was administrated in drinking water. Rats were treated with a combination of four herbal products (Cyperus rotundus, Crocus sativus, Piper nigrum, and Boswellia serrata) mixed with honey at two doses (640 and 1,280 mg/kg) or donepezil (0.5 mg/kg), for 6 weeks. Memory performance on the Morris water maze (MWM) and avoidance behavior in passive avoidance was impaired by hypothyroidism, and brain tissue oxidative damage occurred. Herbal combination and donepezil significantly improved memory impairment, reduced malondialdehyde concentration, and nitric oxide metabolites while increased the thiol contents and catalase and superoxide dismutase enzymes activity in the brain. Our findings suggest that the mixture of herbal products improves learning and memory deficits caused by hypothyroidism, probably by reducing the brain tissue oxidative damage. Practical applications Learning and memory impairment is a common feature of thyroid hormones deficiency. Several studies are showing that hypothyroidism in juvenile and mature rats induces significant cognitive impairment. Likewise, in humans, a close relationship between thyroid hormone deficiency and cognitive impairment has been reported. We used a mixture of herbal products, including Cyperus rotundus, Crocus sativus, Piper nigrum, and Boswellia serrata, to treat hypothyroidism‐induced memory impairment. All these herbs are widely used as a food additive across the world. In Iranian traditional medicine, this herbal combination traditionally used to treat cognitive impairments. Numerous studies have indicated that these herbs show neuroprotective and memory‐enhancing properties. Our finding indicated that a traditionally used herbal combination could potentially use as a treatment of cognitive impairment induced by thyroid hormone deficiency.

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Neuroprotective and cognitive-enhancing effects of the combined extract of Cyperus rotundus and Zingiber officinale

Cited by 51 publications

References 41 publications

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

α-Cyperone Attenuates H2O2-Induced Oxidative Stress and Apoptosis in SH-SY5Y Cells via Activation of Nrf2

Acetylcholinesterase Inhibitors among Zingiber officinale Terpenes—Extraction Conditions and Thin Layer Chromatography-Based Bioautography Studies

The effects of the combination ofCyperus rotundus,Crocus sativus,Piper nigrum,andBoswellia serrataon learning and memory deficit and oxidative damage in brain tissue of hypothyroid rats

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