Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

Okkay, Irmak Ferah; Okkay, Ufuk; Çiçek, Betül; Yilmaz, Aysegul; Yeşilyurt, Fatma; Mendil, Ali Sefa; Hacımüftüoğlu, Ahmet

doi:10.1007/s11033-021-06779-y

Cited by 22 publications

(13 citation statements)

References 42 publications

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“…As expected, 200 µM 6-OHDA exposure significantly impaired the viability of SH -SY5Y cells, with viability as low as 51.4% (Figure 1). The results shown in this study are consistent with previous reports (12,14). Conversely, pretreatment with RES C-Dots significantly increased the viability of SH -SY5Y cells treated with 6-OHDA.…”

Section: Discussionsupporting

confidence: 94%

“…In this report, the neuroprotective effect of RES C-Dots was investigated in an in vitro PD model. 6-OHDA is a potent neurotoxin that enters DANs via the dopamine transporter, accumulates in the cell, and causes oxidative damage leading to cell death of DANs (12). In the present report, the protective effect of RES C-Dots on cell injury induced by 6-OHDA was investigated using an MTT assay.…”

Section: Discussionmentioning

confidence: 91%

“…6-hydroxydopamine (6-OHDA), a potent neurotoxin that damages dopaminergic neurons (DANs), is commonly employed to stimulate in vivo and in vitro experimental PD models. 6-OHDA generates intracellular ROS as well as other free radicals and prevents mitochondria to activate apoptosis cascades (12). The SH-SY5Y cells demonstrate many features of substantia nigra neurons and are so appropriate for utilization as an in vitro model to research the death of DANs (13).…”

Section: Introductionmentioning

confidence: 99%

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Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

CİCEK,

DANIŞMAN

2023

Harran Üniversitesi Tıp Fakültesi Dergisi

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Background: We aimed to investigate the ability of resveratrol carbon dots (RES C-Dots) to protect SH-SY5Y cells from oxido-inflammatory stress and apoptosis caused by 6-hydroxydopamine (6-OHDA). Materials and Methods: In vitro PD model was generated in SH-SY5Y cells by administering of 200 µM 6-OHDA for 24 hours. Different concentrations of RES C-Dots (12.5, 25, and 50 µg/mL) were applied to the cells 30 minutes before administration of 6-OHDA. Results: We observed that application of RES C-Dots prevented cell death induced by 6-OHDA and main-tained cell viability. As expected, RES C-Dots prevented oxidative damage induced by 6-OHDA - by strengthening the total amount of antioxidants and lowering the total amount of oxidants in SH-SY5Y cells. Similarly, RES C-Dots markedly alleviated the secretion of inflammatory factors (TNF-α and IL-1β) promot-ed by 6-OHDA. Furthermore, RES C-Dots prevented apoptosis induced by 6-OHDA by suppressing caspase-3 mRNA expression level. Conclusions: RES C-Dots rescued SH-SY5Y cells from 6-OHDA- induced damage by modulating the oxido-inflammatory and apoptotic response. This report indicates enounces that RES- synthesised C-Dots may have promising curative potential for PD.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

CİCEK,

DANIŞMAN

2023

Harran Üniversitesi Tıp Fakültesi Dergisi

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“…Moreover, our chymotrypsin‐like activity assay demonstrated that REO enhanced the proteasome 20S functions on treatment. Knowing that 6‐OHDA generates ROS‐like superoxide radicals could cause DA neurons degeneration (Ferah Okkay et al, 2021). DA neurons are susceptible to ROS‐induced oxidative stress (Tauffenberger & Magistretti, 2021).…”

Section: Discussionmentioning

confidence: 99%

Rose essential oil diminishes dopaminergic neuron degenerations and reduces α‐synuclein aggregation in Caenorhabditis elegans models of Parkinson's disease

Muhammad

Liu

Wang

et al. 2023

Phytotherapy Research

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Parkinson's disease (P.D.) is the second most progressive neurodegenerative disorder in the elderly. Degeneration of dopaminergic (DA) neurons and α-synuclein (α-Syn) accumulated toxicity is the major contributor to this disease. At present, the disease has no effective treatment. Many recent studies focus on identifying novel therapeutics that provide benefits to stop the disease progression in P.D. patients. Screening novel and effective drugs in P.D. animal models is time-and cost-consuming. Rose Essential Oil (REO) extracted from Rosa Rugosa species (R. Setate Â R. Rugosa). REO contains Citronellol, Geraniol, and Octadiene that possess anti-Aβ, anti-oxidative, and anti-depression-like properties, but no reports have defined the REO effect on P.D. yet. The present study examines the REO neuroprotective potential in transgenic Caenorhabditis elegans P.D. models. We observed that REO reduced α-Syn aggregations and diminished DA neuron degenerations induced by 6-OHDA, reduced food-sensing behavioural disabilities, and prolonged the lifespan of the nematode. Moreover, REO augmented the chymotrypsin-like proteasome and SOD-3 activities. Further, we observed the anti-oxidative role of REO by reducing internal cells ROS.Together, these findings supported REO as an anti-PD drug and may exert its effects by lowering oxidative stress via the anti-oxidative pathway.

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“…In addition, an in vitro study conducted on osteoclast precursors in which it is administered in combination with curcumin has shown that piperine is able to reduce the activity of osteoclasts and increase the inhibition of RANKL-induced osteoclastogenesis [ 11 ]. Bromelain is a set of proteolytic enzymes with a wide range of therapeutic benefits, including an antioxidant activity in vitro [ 46 ] that is also exploited for the treatment of disorders of the musculoskeletal system [ 10 ]. Papain, another proteolytic enzyme, derived from papaya, exerts hepatic detox activity in concert with bromelain.…”

Section: Discussionmentioning

confidence: 99%

Herbal Preparation (Bromelain, Papain, Curcuma, Black Pepper) Enhances Mineralization and Reduces Glucocorticoid-Induced Osteoporosis in Zebrafish

et al. 2021

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Natural foods with antioxidant properties, such as curcuma, papain, bromelain and black pepper, have been indicated as a potential natural therapeutic approach against osteoporosis. Zebrafish are an excellent animal model to study the effects of herbal preparations on osteogenesis and bone metabolism, both in physiological and in pathological conditions. Our study was aimed at evaluating whether curcuma-bromelain-papain-pepper herbal preparation (CHP) administered in embryos and adult fish is capable of promoting bone wellness in physiological and osteoporotic conditions. The effect of CHP has been studied in embryonic osteogenesis and glucocorticoid-induced osteoporosis (GIOP) in an adult fish model in which drug treatment induces a bone-loss phenotype in adult scales very similar to that which characterizes the bones of human patients. CHP prevented the onset of the osteoporotic phenotype in the scales of GIOP in adult zebrafish, with the osteoblastic and osteoclastic metabolic activity maintaining unaltered. CHP is also able to attenuate an already established GIOP phenotype, even if the alteration is in an advanced phase, partially restoring the normal balance of the bone markers alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) and stimulating anabolic reparative processes. The results obtained indicated CHP as a potential integrative antioxidant therapy in human bone-loss diseases.

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Neuroprotective effect of bromelain in 6-hydroxydopamine induced in vitro model of Parkinson’s disease

Cited by 22 publications

References 42 publications

Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

Resveratrol Karbon Noktalarının SH-SY5Y İnsan Nöroblastoma Hücrelerinde 6-OHDA Kaynaklı Nöronal Hücre Ölümü Üzerinde Koruyucu Etkileri

Rose essential oil diminishes dopaminergic neuron degenerations and reduces α‐synuclein aggregation in Caenorhabditis elegans models of Parkinson's disease

Herbal Preparation (Bromelain, Papain, Curcuma, Black Pepper) Enhances Mineralization and Reduces Glucocorticoid-Induced Osteoporosis in Zebrafish

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