Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats

Chen, B.; Gao, Xiaoqing; Yang, Chaoxian; Tan, S.-K.; Sun, Zhiyang; Yan, Naihong; Pang, Yi; Yuan, Meijin; Chen, G.-J.; Xu, Guanghui; Zhang, K.; Yuan, Qionglan

doi:10.1016/j.brainres.2009.05.100

Cited by 84 publications

(66 citation statements)

References 26 publications

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“…Whether those cells were actually integrated into hippocampal circuits is not yet known. Regarding trophic support, studies of ischemic injury found transplanted neural stem/precursor cells to be neuroprotective via glial cell line-derived neurotrophic factor (GDNF) (48,49). Other work using a transgenic model of Alzheimer disease provided evidence that brain-derived neurotrophic factor (BDNF) from transplanted rodent neural stem cells (mNSC) played a significant role in restoring certain cognitive deficits when assessed 1-month posttransplantation (31).…”

Section: Discussionmentioning

confidence: 99%

Rescue of radiation-induced cognitive impairment through cranial transplantation of human embryonic stem cells

Acharya

Christie

Lan

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

114

151

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Cranial irradiation remains a frontline treatment for the control of tumor growth, and individuals surviving such treatments often manifest various degrees of cognitive dysfunction. Radiation-induced depletion of stem/precursor cell pools in the brain, particularly those residing in the neurogenic region of the hippocampus, is believed, in part, to be responsible for these often-unavoidable cognitive deficits. To explore the possibility of ameliorating radiation-induced cognitive impairment, athymic nude rats subjected to head only irradiation (10 Gy) were transplanted 2 days afterward with human embryonic stem cells (hESC) into the hippocampal formation and analyzed for stem cell survival, differentiation, and cognitive function. Animals receiving hESC transplantation exhibited superior performance on a hippocampal-dependent cognitive task 4 months postirradiation, compared to their irradiated surgical counterparts that did not receive hESCs. Significant stem cell survival was found at 1 and 4 months postirradiation, and transplanted cells showed robust migration to the subgranular zone throughout the dentate gyrus, exhibiting signs of neuron morphology within this neurogenic niche. These results demonstrate the capability to ameliorate radiation-induced normal tissue injury using hESCs, and suggest that such strategies may provide useful interventions for reducing the adverse effects of irradiation on cognition.cognition ͉ stem cells ͉ radiotherapy

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Section: Discussionmentioning

confidence: 99%

Rescue of radiation-induced cognitive impairment through cranial transplantation of human embryonic stem cells

Acharya

Christie

Lan

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

114

151

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“…This has been demonstrated in studies where transplanted NPCs expressing the BDNF gene lead to improved functional outcomes relative to control (nonexpressing) NPC recipients [146,147] . Transplantation of GDNFtransfected NPCs not only improves functional outcomes, but also increases BDNF expression, ultimately leading to improved cell survival [148] . Hypoxia-induction factor (HIF)-transfected cells were shown to improve functional outcomes, and increase the proliferation of endothelial cells, suggesting increased angiogenesis [149] .…”

Section: Combinatorial Aproaches To Enhance Stroke Recoverymentioning

confidence: 99%

Role of neural precursor cells in promoting repair following stroke

Dibajnia

Morshead

2012

Acta Pharmacol Sin

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Stem cell-based therapies for the treatment of stroke have received considerable attention. Two broad approaches to stem cell-based therapies have been taken: the transplantation of exogenous stem cells, and the activation of endogenous neural stem and progenitor cells (together termed neural precursors). Studies examining the transplantation of exogenous cells have demonstrated that neural stem and progenitor cells lead to the most clinically promising results. Endogenous activation of neural precursors has also been explored based on the fact that resident precursor cells have the inherent capacity to proliferate, migrate and differentiate into mature neurons in the uninjured adult brain. Studies have revealed that these neural precursor cell behaviours can be activated following stroke, whereby neural precursors will expand in number, migrate to the infarct site and differentiate into neurons. However, this innate response is insufficient to lead to functional recovery, making it necessary to enhance the activation of endogenous precursors to promote tissue repair and functional recovery. Herein we will discuss the current state of the stem cell-based approaches with a focus on endogenous repair to treat the stroke injured brain.

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“…27 A possible role for glial cell-derived neurotrophic factor in cell survival has been demonstrated by administration of this cytokine after middle cerebral artery occlusion in rats and by genetically modifying NPCs with glial cell-derived neurotrophic factor before transplantation in rats after middle cerebral artery occlusion. 28,29 These results suggest that trophic factor mechanism rather than cell replacement per se, contribute predominantly to the behavioural improvement in animal models. Therefore, the combination of stimulating endogenous neurogenesis with cytokines and stem cell technology will likely have a huge impact in the future treatment of stroke patients.…”

Section: Early Clinical Trials For Cell Transplantation With Differenmentioning

confidence: 90%

Progress and prospects: stem cells and neurological diseases

2010

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Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats

Cited by 84 publications

References 26 publications

Rescue of radiation-induced cognitive impairment through cranial transplantation of human embryonic stem cells

Rescue of radiation-induced cognitive impairment through cranial transplantation of human embryonic stem cells

Role of neural precursor cells in promoting repair following stroke

Progress and prospects: stem cells and neurological diseases

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