Neuroprotective Effect of Oxyresveratrol from Smilacis Chinae Rhizome on Amyloid .BETA. Protein (25-35)-Induced Neurotoxicity in Cultured Rat Cortical Neurons

Ban, Ju Yeon; Jeon, Se Young; Nguyen, Thi Thuy Ha; Bae, KiHwan; Song, Kyung‐Sik; Seong, Yeon Hee

doi:10.1248/bpb.29.2419

Cited by 69 publications

(43 citation statements)

References 37 publications

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“…The Ethical Committee of Ataturk University approved the study protocol (B.30.2.ATA.0.23.85-73). Primary rat cerebral cortex neuron cultures were prepared using rat foetuses as described previously (Ban et al 2006). Briefly, a total of nine new-born Sprague-Dawley rats were used in the study.…”

Section: Healthy Neuron Cell Culturesmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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The main objective of this study is to investigate the cytotoxic, genotoxic and antioxidant properties of zingiberene (ZBN) in an in vitro rat brain cell culture study. The cytotoxic effect was determined against the rat neuron and N2a neuroblastoma (N2a-NB) cell lines using the 3,(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, while the antioxidant activity was assessed using the total antioxidant capacity (TAC) and total oxidative stress (TOS) assays. The effects on DNA damage were also evaluated in this study by the single cell gel electrophoresis assay. The results indicated that ZBN has an anti-proliferative activity suppressing the proliferation of N2a-NB cells at concentrations over 50 mg L -1 and neuron cells at concentrations over 150 mg L -1. In addition, ZBN treatments at higher doses (B50 mg L -1 ) led to increases of TOS levels in N2a-NB cell cultures. However 25 mg L -1 of ZBN treatment caused increases of TAC levels in cultured neuron and N2a-NB cell cultures while ZBN at doses of 10-400 mg L -1 did not increase the number of total damage score in both cell lines. This study clearly indicates that ZBN has a significant potential to be used as a natural anticancer agent in cultured N2a-NBs.

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Section: Healthy Neuron Cell Culturesmentioning

confidence: 99%

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

et al. 2014

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“…15 Moreover, we have demonstrated that the suppression of intracellular [Ca 2+ ] i elevation by numerous antioxidants such as oxyresveratrol, gallic acid, catechin, and epicatechin is primarily responsible for the ability of these compounds to prevent Ab (25-35)-induced neuronal death. 26,[38][39][40] Therefore, results from the present study suggested that the antidementia effect of I. latifolia might be attributable to the prevention of apoptosis by suppressing intracellular [Ca 2+ ] i increases and ROS generation due to the antioxidant components of this plant.…”

Section: Discussionmentioning

confidence: 58%

Ilex latifoliaPrevents AmyloidβProtein (25–35)-Induced Memory Impairment by Inhibiting Apoptosis and Tau Phosphorylation in Mice

Kim

Lee

Jang

et al. 2015

Journal of Medicinal Food

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Ilex latifolia Thunb. (Aquifoliaceae), a Chinese bitter tea called ''kudingcha,'' has been widely consumed as a health beverage and found to possess antioxidant, antidiabetic, antihypertensive, anti-inflammatory, and anti-ischemic activities. The aim of the present study was to investigate the neuroprotective effects of an ethanol extract of I. latifolia against amyloid b protein (Ab)-induced memory impairment in mice and neurotoxicity in cultured rat cortical neurons. Memory impairment in mice was induced by intracerebroventricular injection of 15 nmol Ab (25-35) and measured by the passive avoidance test and Morris water maze test. Chronic administration of I. latifolia (25-100 mg/kg, p.o.) significantly prevented Ab (25-35)-induced memory loss. I. latifolia also prevented the decrease of glutathione concentrations, increased lipid peroxidation, expression of phosphorylated tau (p-tau), and changes in apoptosis-associated proteins in the memory-impaired mouse brain. Exposure of cultured cortical neurons to 10 lM Ab (25-35) for 36 h induced neuronal apoptotic death. The neuronal cell death, elevation of intracellular Ca 2+ concentration, generation of reactive oxygen species, and expression of proapoptotic proteins caused by in the cultured neurons were inhibited by treatment with I. latifolia (1-50 lg/mL). These results suggest that I. latifolia may have a possible therapeutic role in managing cognitive impairment associated with Alzheimer's disease. The underlying mechanism might involve the antiapoptotic effects mediated by antioxidant activity and inhibition of p-tau formation.

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“…Primary rat cerebral cortex neuron cultures were prepared using rat foetuses as described previously (Ban et al 2006). Briefly, a total of nine new-born Sprague-Dawley rats were used in the study.…”

Section: Cell Culturementioning

confidence: 99%

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

et al. 2014

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Sesquiterpenes have attracted much interest with respect to their protective effect against oxidative damage that may be the cause of many diseases including several neurodegenerative disorders and cancer. Our previous unpublished work suggested that cyclosativene (CSV), a tetracyclic sesquiterpene, has antioxidant and anticarcinogenic features. However, little is known about the effects of CSV on oxidative stress induced neurotoxicity. We used hydrogen peroxide (H 2 O 2 ) exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of CSV in H 2 O 2 -induced toxicity in newborn rat cerebral cortex cell cultures for the first time. For this aim, MTT and lactate dehydrogenase release assays were carried out to evaluate cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to evaluate oxidative changes. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels, the single cell gel electrophoresis (or Comet assay) was also performed for measuring the resistance of neuronal DNA to H 2 O 2 -induced challenge. Our results showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage (Comet assay) increased in the H 2 O 2 alone treated cultures. But pretreatment of CSV suppressed the cytotoxicity, genotoxicity and oxidative stress which were increased by H 2 O 2 . On the basis of these observations, it is suggested that CSV as a natural product with an antioxidant capacity in mitigating oxidative injuries in the field of neurodegenerative disorders.

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Neuroprotective Effect of Oxyresveratrol from Smilacis Chinae Rhizome on Amyloid .BETA. Protein (25-35)-Induced Neurotoxicity in Cultured Rat Cortical Neurons

Abstract: We previously reported that] c , and then by inhibiting glutamate release and ROS generation. Furthermore, these effects of oxyresveratrol may be associated with the neuroprotective effect of Smilacis chinae rhizome.

Cited by 69 publications

References 37 publications

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Cytotoxicity and genotoxicity of zingiberene on different neuron cell lines in vitro

Ilex latifoliaPrevents AmyloidβProtein (25–35)-Induced Memory Impairment by Inhibiting Apoptosis and Tau Phosphorylation in Mice

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

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