Neuroprotective Effect of Polyherbal Recipe Containing Ginger, Chinese Date, and Wood Ear Mushroom against Ischemic Stroke with Metabolic Syndrome Condition via Epigenetic Modification of Inflammation and Oxidative Stress

Abstract

Currently, the prevalence of stroke with metabolic syndrome (MetS) is increasing and the current therapeutic efficiency is still limited. Therefore, the applications of herbal recipes have gained much attention. The polyherbal recipe containing ginger, Chinese date, and wood ear mushroom is reputed for atherosclerosis and stroke prevention. It has been long-term consumed without scientific support. Therefore, this study was carried out to determine the neuroprotective effect and its mechanisms in animal model of ischemic stroke with MetS. Male Wistar rats weighing 180-220 g were exposed to a 16-week high-fat high-carbohydrate feeding. The rats with the MetS characteristic were exposed to a temporary occlusion of the right middle cerebral artery (MCAO) for 90 minutes. They were orally fed with the polyherbal recipe (GCJ) at the doses of 100, 200, and 300 mg/kg BW for 21 days and assessed the neurological deficit, ion volume, cortical neuron density in the cerebral cortex, oxidative stress status, inflammation, and expressions of histone deacetylase 3 (HDAC3) and DNA methyltransferase 1 (DNMT1). The results showed that GCJ significantly improved all mentioned parameters. Therefore, GCJ is the potential neuroprotectant against ischemic stroke with MetS. The underlying mechanisms may involve the reduction of oxidative stress, inflammation, and the modification of epigenetic mechanism via the reduction of HDAC3 and DNMT1. However, further clinical investigation is essential to confirm this positive modulation effect.

The Polyherbal Functional Ingredient Containing Ginger, Chinese Date, and Wood Ear Mushroom Protects against Dementia following Metabolic Syndrome

“…Each rat was exposed to a 90-minute-occlusion of the right middle cerebral artery with a coated silicone nylon (no.4, dimension 1.5, Taiwan). At the end of an occlusion period, a nylon was withdrawn to allow the reperfusion injury [ 16 , 17 ]. Similar processes were also carried out for the sham operation group, except that no filament insertion was performed.…”

“…Accumulative lines of evidence also reveal that the herbs just mentioned also possess the biological assessment related with the pathophysiology of stroke, metabolic syndrome, and memory impairment such as antioxidant and anti-inflammation [ 7 – 15 ]. In addition, our recent study also clearly demonstrates that GCJ exhibits a cerebroprotective effect against ischemia-stroke with metabolic syndrome (MetS) [ 16 ]. Owing to the potential benefits of the aforementioned herbs on vascular, the buffering capacity on oxidative stress and inflammation together with the cerebroprotective effect against ischemic stroke, the positive variation effect of GCJ on memory impairment.…”

Improving hydrocarbon toxicity tolerance in poultry: role of genes and antioxidants

“…Curcumin and ginger were found to induce a protective effect by upregulating the activities of antioxidative enzymes, thereby modulating oxidative stress ( Lin et al, 2019 ; Gao et al, 2022 ). A reduction in inflammation from oxidative stress was seen in mice administered a polyherbal mix of ginger, Chinese date, and wood ear mushroom ( Nakyam et al, 2022 ).…”