Neuroprotective Effect of Quercetin Against Hydrogen Peroxide-induced Oxidative Injury in P19 Neurons

Abstract

Oxidative stress is implicated in neuronal death in a variety of neurodegenerative diseases. In the present study, P19 neurons obtained by the differentiation procedure from mouse teratocarcinoma P19 cells were used to investigate the ability of quercetin, a plant-derived flavonoid, to prevent neuronal death induced by exposure to 150 μM or 1.5 mM hydrogen peroxide (H(2)O(2)) for 24 h. Quercetin treatment improved viability of P19 neurons exposed to both types of oxidative injury. During the modest oxidative stress, quercetin diminished generation of reactive oxygen species (ROS) and prevented H(2)O(2)-induced nuclear condensation, increase in caspase 3/7 activity and rise in poly(APD-ribose) polymerase expression. Expression of Bcl-2 family members Bax and Bcl-2 was not affected by quercetin treatment at both the transcriptional and translational levels. During the severe oxidative injury, quercetin prevented H(2)O(2)-induced rise in ROS accumulation and changes in plasma membrane integrity and nuclear morphology. The obtained results suggest that neuroprotective effects of quercetin are related to its antioxidative action and prevention of events associated with programmed cell death cascade. In the light of these findings, one might assume beneficial effects of quercetin for the prevention of oxidative stress-driven neuronal loss in human aging and age-related neurodegenerative diseases.

Protective Effect of Quercetin on the Development of Preimplantation Mouse Embryos against Hydrogen Peroxide-Induced Oxidative Injury

“…Previous research showed that supplementation of quercetin in culture media effectively prevented H 2 O 2 -induced ROS accumulation and inhibited apoptotic signals in P19 neuron cells [38]. Moreover, quercetin also markedly suppressed apoptosis in H9c2 cardiomyoblast cells through the reduction of intracellular ROS [39].…”

The Role of Brain-Derived Neurotrophic Factor in Comorbid Depression: Possible Linkage with Steroid Hormones, Cytokines, and Nutrition

“…For example, quercetin, a typical flavonoid, rescues neuronal cells against oxidative stress induced by H 2 O 2 exposure through the reduction of reactive oxygen species (114). Nuclear factor E2-related factor 2 (Nrf2)/antioxidant response element (ARE) is critical for the protective action of flavonoids under oxidative insult and is expected to be the potential target for improvement of neurodegeneration (115, 116).…”

Atomic force microscopy reveals new biomarkers for monitoring subcellular changes in oxidative injury: neuroprotective effects of quercetin at the nanoscale

“…As previously reported, quercetin prevents H 2 O 2 -provoked upregulation of ROS production. [30] Here, we analysed levels of reduced glutathione (GSH), one of the major non-enzymatic intracellular antioxidants, as oxidative stress may alter reservoirs of intracellular antioxidant systems. Exposure to H 2 O 2 depleted GSH to 55.7% of the vehicle-treated group, but in the presence of quercetin intracellular GSH content was not restored.…”