Neuroprotective Effects of Hydroalcoholic Extract of Ocimum sanctum Against H2O2 Induced Neuronal Cell Damage in SH-SY5Y Cells via Its Antioxidative Defence Mechanism

Venuprasad, M. P.; Kandikattu, Hemanth Kumar; Khanum, Farhath

doi:10.1007/s11064-013-1128-7

Cited by 49 publications

(26 citation statements)

References 45 publications

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“…Dose-dependent decreases in SBs were seen with all three (though even the lowest concentration tested was high). An extract of Ocimum sanctum (a form of basil) was tested by Venuprasad et al [41] on human neuroblastoma cells; it protected against H 2 O 2 -induced SBs (at a high concentration).…”

Section: Resultsmentioning

confidence: 99%

Polyphenols and DNA Damage: A Mixed Blessing

2016

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Polyphenols are a very broad group of chemicals, widely distributed in plant foods, and endowed with antioxidant activity by virtue of their numerous phenol groups. They are widely studied as putative cancer-protective agents, potentially contributing to the cancer preventive properties of fruits and vegetables. We review recent publications relating to human trials, animal experiments and cell culture, grouping them according to whether polyphenols are investigated in whole foods and drinks, in plant extracts, or as individual compounds. A variety of assays are in use to study genetic damage endpoints. Human trials, of which there are rather few, tend to show decreases in endogenous DNA damage and protection against DNA damage induced ex vivo in blood cells. Most animal experiments have investigated the effects of polyphenols (often at high doses) in combination with known DNA-damaging agents, and generally they show protection. High concentrations can themselves induce DNA damage, as demonstrated in numerous cell culture experiments; low concentrations, on the other hand, tend to decrease DNA damage.

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Section: Resultsmentioning

confidence: 99%

Polyphenols and DNA Damage: A Mixed Blessing

2016

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“…H 2 O 2 diffuses into cells from the extracellular space, so enzymes such as catalase and glutathione peroxidase would have actively eliminated it (Bienert and Chaumont 2014; Bienert et al 2006; Dringen and Hamprecht 1997; Dringen et al 2005; Dunning et al 2013; Venuprasad et al 2013). The contrasting cell-dependent increase in H 2 O 2 from menadione is also expected because menadione induces superoxide production by the mitochondria, which is then converted to hydrogen peroxide (Buckman et al 1993; Fukui et al 2012; Thor et al 1982).…”

Section: Discussionmentioning

confidence: 99%

Altered cytotoxicity of ROS-inducing compounds by sodium pyruvate in cell culture medium depends on the location of ROS generation

et al. 2015

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Induction of oxidative stress by drugs and other xenobiotics is an important mechanism of cytotoxicity. However, in vitro studies on the relationship between oxidative stress and cytotoxicity in cultured cells is frequently complicated by the fact that cell culture medium components affect reactive oxygen species (ROS) exposures in ways that vary with the mode of ROS production. The objectives of this study were to first determine the mode of ROS induction by certain model compounds when they are applied to cultured cells, and then to determine how ROS induction and cytotoxicity were affected by the ROS-quenching medium component pyruvate. Three compounds, eseroline, benserazide, and pyrogallol induced H2O2 in cell culture media independent of cells. However, another compound, menadione, induced H2O2 in a manner largely dependent on the MDA-MB-231 breast cancer cells used in this study, which is consistent with its known mechanism of inducing ROS through intracellular redox cycling. 1 mM pyruvate, as well as catalase, reduced the H2O2 in culture wells with each ROS inducer tested but it only reduced the cytotoxicity of cell-independent inducers. It reduced the cytotoxicity of benserazide and pyrogallol >10-fold and of eseroline about 2.5-fold, but had no effect on menadione cytotoxicity. From this data, it was concluded that depending on the mechanism of ROS induction, whether intra- or extracellular, a ROS-quenching medium component such as pyruvate will differentially affect the net ROS-induction and cytotoxicity of a test compound.

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“…In addition, mitochondria plays a central role in apoptosis and the overgeneration of ROS induced by oxidative stress could lead to the MMP loss, which makes a contribution to open the mitochondrial permeability transition pore (Beal, 2005;Venuprasad et al, 2013). However, even worse is that the destruction of the mitochondria integrity could cause the increasing consumption of ATP and the generation of ROS (Dumont and Beal, 2011).…”

Section: Discussionmentioning

confidence: 99%