Neuroprotective effects of Kukoamine B against hydrogen peroxide-induced apoptosis and potential mechanisms in SH-SY5Y cells

Hu, Xiaolong; Niu, Yixuan; Zhang, Qiao; Tian, Xing; Gao, Lingyue; Guo, Liping; Meng, Wei; Zhao, Qingchun

doi:10.1016/j.etap.2015.06.017

Cited by 56 publications

(26 citation statements)

References 41 publications

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“…Annexin V FITC‐PI double staining assay was used to distinguish the features of apoptotic and necrotic cells in response to H 2 O 2 . Annexin V probe of FITC stain has a robust affinity for phosphotidylserine surface from the inner plasma membrane, which invert during apoptosis . Double staining of the cells with PI (propidium iodide) was used to identify the late apoptotic and necrotic condition, marked by loss of cell membrane integrity .…”

Section: Resultssupporting

confidence: 88%

“…This oxidative stress was observed to be associated with increase in intracellular ROS . Previous studies have also reported that lower level of H 2 O 2 induces a temporary growth arrest, whilst high concentration induces necrotic death . In current study, inhibitory concentration (IC 50 ) of hydrogen peroxide (0.42 m m ) was estimated graphically from a plot of cell viability vs H 2 O 2 concentration (Figure ).…”

Section: Discussionmentioning

confidence: 99%

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Comparative evaluation of four triterpenoid glycoside saponins of bacoside A in alleviating sub-cellular oxidative stress of N2a neuroblastoma cells

Bhardwaj

Jain

Mathur

2018

Journal of Pharmacy and Pharmacology

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of four triterpenoid glycoside saponins of bacoside A in alleviating sub-cellular oxidative stress of N2a neuroblastoma cells

Bhardwaj

Jain

Mathur

2018

Journal of Pharmacy and Pharmacology

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“…Several studies have investigated the mechanisms of H 2 O 2 ‐induced damage in central nervous cells using the SH‐SY5Y cell line as in vitro model (Hu et al, ). Prior to investigating the neuroblastoma cell protection effect of the isolates on the SHSY5Y cell model, we determined the viability of cells pre‐treated with increasing doses of the test compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative stress can cause damage to the central nervous cells. Therefore, H 2 O 2 ‐induced damage in the neuron‐like cells, such as the human neuroblastoma SH‐SY5Y cells, is used to explore the neuroprotective capacity of natural compounds in NDDs (Hu et al, ).…”

Section: Methodsmentioning

confidence: 99%

A network pharmacology study on the triterpene saponins fromMedicago sativaL. for the treatment of Neurodegenerative diseases

Liu

Huang³

et al. 2019

J Food Biochem

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Neurodegenerative diseases (NDDs) are characterized by progressive and irreversible, is a kind of complex illnesses, and the long‐term therapy which is frequently associated with adverse side effects. Medicago sativa L., widely consumed as a vegetable, has the effects of improving memory and relieving central nervous system diseases. However, there are less studies on its specific mechanism for NDDs. In this investigation, we applied a method of network pharmacology, which combined molecular docking and network analysis to decipher the mechanisms of M. sativa in NDDs. The pharmacological system generated 55 triterpene saponins from M. sativa, and predicted 27 potential targets with 100 pathways in the treatment of NDDs. As a result, 13 compounds, 10 target proteins, and 6 signaling pathways were found to play important roles in the treatment of NDDs. In addition, in vitro experiments of isolates confirmed activities for NDDs, which were consistent with the results of network pharmacology prediction. Practical applications Medicago sativa L. has been widely consumed as a vegetable, which possesses many nutritional components. As a functional food stuff, M. sativa can improve human health, such as memory improving activities, relieving central nervous system diseases, immunomodulatory, antioxidant, anticancer, and anti‐inflammatory. In this article, the mechanism of triterpene saponins from M. sativa against NDDs was successfully predicted by network pharmacology method. The results will serve as a reference of M. sativa against NDDs.

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“…In primary cortical cells, H 2 O 2 was reported to have activated both ERK1/ 2 and p-38 MAP kinase signaling protein in a concentration and time dependent manner [49]. Studies on plant bioactives such as kukoamine B and loganin in human neuroblastoma ShSy5y cells, have been reported to provide neuroprotection against H 2 O 2 induced oxidative stress via attenuation of phospho-ERK1/2 [50,51]. Some earlier studies in differentiated and undifferentiated Shsy5y cells have shown that treatment with H 2 O 2 increased the phosphorylation of ERK1/2 in a time and concentration dependent manner (up to 1.25 mM H 2 O 2 ) where it increased as the concentration of H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

et al. 2017

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Background: Peltophorum pterocarpum is a plant from the family of Leguminosae and a tropical tree found in South East Asia region. The leaves of the plant is rich in anti-oxidant polyphenols. Traditionally this plant was used to cure intestinal disorders and as a relief for sprain, bruise, muscular pain and sores and for pain during child birth. Since oxidative stress is a major contributing factor towards neurodegenerative diseases the leaves of this plant was explored for its neuroprotective properties. Method: In this study, we report the neuroprotective properties of the ethanolic extract of Peltophorum pterocarpum leaf against oxidative stress induced cell death by H 2 O 2 , in an in vitro model of neuronally differentiated IMR32 neuroblastoma cells. Hydrogen peroxide was used as an inducer for oxidative stress. Cell viability was determined using MTT assay, apoptosis studies were carried out using Annexin V-FITC and Caspase 3 assays, mitochondrial membrane potential was examined using JC1 staining. The oxidative stress induced by H 2 O 2 was determined by quantifying the intracellular reactive oxygen species production using DCF-DA staining. Mitogen activated protein kinase signaling pathway of the neuroprotective effect of PTE was also elucidated since oxidative stress activates this pathway signaling.

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Neuroprotective effects of Kukoamine B against hydrogen peroxide-induced apoptosis and potential mechanisms in SH-SY5Y cells

Cited by 56 publications

References 41 publications

Comparative evaluation of four triterpenoid glycoside saponins of bacoside A in alleviating sub-cellular oxidative stress of N2a neuroblastoma cells

Comparative evaluation of four triterpenoid glycoside saponins of bacoside A in alleviating sub-cellular oxidative stress of N2a neuroblastoma cells

A network pharmacology study on the triterpene saponins fromMedicago sativaL. for the treatment of Neurodegenerative diseases

Neuroprotective effects of Peltophorum pterocarpum leaf extract against hydrogen peroxide induced oxidative stress and cytotoxicity

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