Neuroprotective Effects of Lactobacillus plantarum PS128 in a Mouse Model of Parkinson’s Disease: The Role of Gut Microbiota and MicroRNAs

Abstract

Parkinson’s disease (PD) is a neurodegenerative disease characterized by motor deficits and marked neuroinflammation in various brain regions. The pathophysiology of PD is complex and mounting evidence has suggested an association with the dysregulation of microRNAs (miRNAs) and gut dysbiosis. Using a rotenone-induced PD mouse model, we observed that administration of Lactobacillus plantarum PS128 (PS128) significantly improved motor deficits in PD-like mice, accompanied by an increased level of dopamine, reduced dopaminergic neuron loss, reduced microglial activation, reduced levels of inflammatory factors, and enhanced expression of neurotrophic factor in the brain. Notably, the inflammation-related expression of miR-155-5p was significantly upregulated in the proximal colon, midbrain, and striatum of PD-like mice. PS128 reduced the level of miR-155-5p, whereas it increased the expression of suppressor of cytokine signaling 1 (SOCS1), a direct target of miR-155-5p and a critical inhibitor of the inflammatory response in the brain. Alteration of the fecal microbiota in PD-like mice was partially restored by PS128 administration. Among them, Bifidobacterium, Ruminiclostridium_6, Bacteroides, and Alistipes were statistically correlated with the improvement of rotenone-induced motor deficits and the expression of miR-155-5p and SOCS1. Our findings suggested that PS128 ameliorates motor deficits and exerts neuroprotective effects by regulating the gut microbiota and miR-155-5p/SOCS1 pathway in rotenone-induced PD-like mice.

Lactiplantibacillus (Lactobacillus) plantarum as a Complementary Treatment to Improve Symptomatology in Neurodegenerative Disease: A Systematic Review of Open Access Literature

“…A total of 17 studies were conducted using different animal models. Of these, 4 focused on AD [ 95 , 96 , 97 , 98 ] ( n = 4); 7 focused on PD [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ] ( n = 7); 1 analyzed MS [ 106 ], ( n = 1); 3 studied oxidative stress [ 107 , 108 , 109 ] ( n = 3); 1 on cognitive impairment and oxidative stress [ 110 ] ( n = 1); and 1 on neurodegenerative process [ 111 ] ( n = 1). All of them addressed the intervention with Lactobacillus plantarum or Lactiplantibacillus plantarum for the improvement of symptoms associated with neurodegenerative pathologies.…”

“…Lee et al (2023) conducted a study in a mouse model of PD [ 100 ]. Lactobacillus plantarum PS128 was administered for 6 weeks and the results showed a significant reduction in motor deficits and higher dopamine levels (PS128 significantly increased DA levels vs. ROT group ( p < 0.05)).…”

“…Evidence suggests that the use of Lactiplantibacillus plantarum significantly reduces motor deficits. It also reduces levels of inflammatory factors and maintains neuroprotective effects on dopaminergic neurons, both in vivo and in vitro [ 99 , 100 , 101 , 102 , 103 , 104 , 105 ].…”

Pesticides in Foods: Towards Bioremediation Biocatalysts?

“…In addition, they even promote plant growth and fulfill the various roles of agrochemicals [41]. At the other end of the pesticide contamination chain, they can exhibit a protective effect on animals with pesticide-induced diseases [42,43]. In between, they can decrease intestinal absorption of pesticides by adsorbing the compounds on their surface [44,45], and of course, they can participate in pesticide degradation [23].…”

Whole Genome Sequencing of the Novel Probiotic Strain Lactiplantibacillus plantarum FCa3L

“…Probiotics are defined as living organisms which, when administered in adequate amounts, confer a health benefit to the host [ 11 ]. The incomplete list of disorders which benefit from the application of specific probiotic strains of L. plantarum comprises irritable bowel syndrome [ 12 ], chronic diarrhea [ 13 ], atopic dermatitis [ 14 ], vaginal infections [ 15 ], cardiovascular disease [ 16 ], cancers [ 17 , 18 ], and neurodegenerative diseases [ 19 , 20 ]. Health-promoting effects of L. plantarum are based on its promising biological activities, such as ability to alter intestinal flora and inhibit the growth of potential pathogens, modulation of host immune responses, antioxidant, antimutagenic, and antihypertensive activities [ 8 , 16 ].…”